LOCUS       40924_20661        9376 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU53, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 9376)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz 
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 
            Niedersachsen 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             36..1475
                     /codon_start=1
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1491..2411
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2463..3542
                     /codon_start=1
                     /label=LuxA
                     /note="LuxA luciferase subunit"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3560..4540
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4722..5831
                     /codon_start=1
                     /label=LuxE
                     /note="LuxE"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      5854..5940
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(6478..7425)
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(7473..7695)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(8187..8281)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8314..9171)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9172..9276)
                     /label=AmpR promoter
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacatgac taaaaaaatt tcattcatta
       61 ttaacggcca ggttgaaatc tttcccgaag gtgatgattt agtgcaatcc attaattttg
      121 gtgataatag tgtttacctg ccaatattga atgactctca tgtaaaaaac attattgatt
      181 gtaatggaaa taacgaatta cggttgcata acattgtcaa ttttctctat acggtagggc
      241 aaagatggaa aaatgaagaa tactcaagac gcaggacata cattcgtgac ttaaaaaaat
      301 atatgggata ttcagaagaa atggctaagc tagaggccaa ttggatatct atgattttat
      361 gttctaaagg cggcctttat gatgttgtag aaaatgaact tggttctcgc catatcatgg
      421 atgaatggct acctcaggat gaaagttatg ttcgggcttt tccgaaaggt aaatctgtac
      481 atctgttggc aggtaatgtt ccattatctg ggatcatgtc tatattacgc gcaattttaa
      541 ctaagaatca gtgtattata aaaacatcgt caaccgatcc ttttaccgct aatgcattag
      601 cgttaagttt tattgatgta gaccctaatc atccgataac gcgctcttta tctgttatat
      661 attggcccca ccaaggtgat acatcactcg caaaagaaat tatgcaacat gcggatgtta
      721 ttgtcgcttg gggagggcca gatgcgatta attgggcggt agagcatgcg ccatcttatg
      781 ctgatgtgat taaatttggt tctaaaaaga gtctttgcat tatcgataat cctgttgatt
      841 tgacgtccgc agcgacaggt gcggctcatg atgtttgttt ttacgatcag cgagcttgtt
      901 tttctgccca aaacatatat tacatgggaa atcattatga ggaatttaag ttagcgttga
      961 tagaaaaact taatctatat gcgcatatat taccgaatgc caaaaaagat tttgatgaaa
     1021 aggcggccta ttctttagtt caaaaagaaa gcttgtttgc tggattaaaa gtagaggtgg
     1081 atattcatca acgttggatg attattgagt caaatgcagg tgtggaattt aatcaaccac
     1141 ttggcagatg tgtgtacctt catcacgtcg ataatattga gcaaatattg ccttatgttc
     1201 aaaaaaataa gacgcaaacc atatctattt ttccttggga gtcatcattt aaatatcgag
     1261 atgcgttagc attaaaaggt gcggaaagga ttgtagaagc aggaatgaat aacatatttc
     1321 gagttggtgg atctcatgac ggaatgagac cgttgcaacg attagtgaca tatatttctc
     1381 atgaaaggcc atctaactat acggctaagg atgttgcggt tgaaatagaa cagactcgat
     1441 tcctggaaga agataagttc cttgtatttg tcccataata ggtaaaaagt atggaaaatg
     1501 aatcaaaata taaaaccatc gaccacgtta tttgtgttga aggaaataaa aaaattcatg
     1561 tttgggaaac gctgccagaa gaaaacagcc caaagagaaa gaatgccatt attattgcgt
     1621 ctggttttgc ccgcaggatg gatcattttg ctggtctggc ggaatattta tcgcggaatg
     1681 gatttcatgt gatccgctat gattcgcttc accacgttgg attgagttca gggacaattg
     1741 atgaatttac aatgtctata ggaaagcaga gcttgttagc agtggttgat tggttaacta
     1801 cacgaaaaat aaataacttc ggtatgttgg cttcaagctt atctgcgcgg atagcttatg
     1861 caagcctatc tgaaatcaat gcttcgtttt taatcaccgc agtcggtgtt gttaacttaa
     1921 gatattctct tgaaagagct ttagggtttg attatctcag tctacccatt aatgaattgc
     1981 cgaataatct agattttgaa ggccataaat tgggtgctga agtctttgcg agagattgtc
     2041 ttgattttgg ttgggaagat ttagcttcta caattaataa catgatgtat cttgatatac
     2101 cgtttattgc ttttactgca aataacgata attgggtcaa gcaagatgaa gttatcacat
     2161 tgttatcaaa tattcgtagt aatcgatgca agatatattc tttgttagga agttcgcatg
     2221 acttgagtga aaatttagtg gtcctgcgca atttttatca atcggttacg aaagccgcta
     2281 tcgcgatgga taatgatcat ctggatattg atgttgatat tactgaaccg tcatttgaac
     2341 atttaactat tgcgacagtc aatgaacgcc gaatgagaat tgagattgaa aatcaagcaa
     2401 tttctctgtc ttaaaatcta ttgagatatt ctatcactca aatagcaata taaggactct
     2461 ctatgaaatt tggaaacttt ttgcttacat accaacctcc ccaattttct caaacagagg
     2521 taatgaaacg tttggttaaa ttaggtcgca tctctgagga gtgtggtttt gataccgtat
     2581 ggttactgga gcatcatttc acggagtttg gtttgcttgg taacccttat gtcgctgctg
     2641 catatttact tggcgcgact aaaaaattga atgtaggaac tgccgctatt gttcttccca
     2701 cagcccatcc agtacgccaa cttgaagatg tgaatttatt ggatcaaatg tcaaaaggac
     2761 gatttcggtt tggtatttgc cgagggcttt acaacaagga ctttcgcgta ttcggcacag
     2821 atatgaataa cagtcgcgcc ttagcggaat gctggtacgg gctgataaag aatggcatga
     2881 cagagggata tatggaagct gataatgaac atatcaagtt ccataaggta aaagtaaacc
     2941 ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt ggctgaatca gcttcgacga
     3001 ctgagtgggc tgctcaattt ggcctaccga tgatattaag ttggattata aatactaacg
     3061 aaaagaaagc acaacttgag ctttataatg aagtggctca agaatatggg cacgatattc
     3121 ataatatcga ccattgctta tcatatataa catctgtaga tcatgactca attaaagcga
     3181 aagagatttg ccggaaattt ctggggcatt ggtatgattc ttatgtgaat gctacgacta
     3241 tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag tggcgtgact
     3301 ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagttac gaaatcaatc
     3361 ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa agacattgat gctacaggaa
     3421 tatcaaatat ttgttgtgga tttgaagcta atggaacagt agacgaaatt attgcttcca
     3481 tgaagctctt ccagtctgat gtcatgccat ttcttaaaga aaaacaacgt tcgctattat
     3541 attagctaag gagaaagaaa tgaaatttgg attgttcttc cttaacttca tcaattcaac
     3601 aactgttcaa gaacaaagta tagttcgcat gcaggaaata acggagtatg ttgataagtt
     3661 gaattttgaa cagattttag tgtatgaaaa tcatttttca gataatggtg ttgtcggcgc
     3721 tcctctgact gtttctggtt ttctgctcgg tttaacagag aaaattaaaa ttggttcatt
     3781 aaatcacatc attacaactc atcatcctgt ccgcatagcg gaggaagctt gcttattgga
     3841 tcagttaagt gaagggagat ttattttagg gtttagtgat tgcgaaaaaa aagatgaaat
     3901 gcattttttt aatcgcccgg ttgaatatca acagcaacta tttgaagagt gttatgaaat
     3961 cattaacgat gctttaacaa caggctattg taatccagat aacgattttt atagcttccc
     4021 taaaatatct gtaaatcccc atgcttatac gccaggcgga cctcggaaat atgtaacagc
     4081 aaccagtcat catattgttg agtgggcggc caaaaaaggt attcctctca tctttaagtg
     4141 ggatgattct aatgatgtta gatatgaata tgctgaaaga tataaagccg ttgcggataa
     4201 atatgacgtt gacctatcag agatagacca tcagttaatg atattagtta actataacga
     4261 agatagtaat aaagctaaac aagagacgcg tgcatttatt agtgattatg ttcttgaaat
     4321 gcaccctaat gaaaatttcg aaaataaact tgaagaaata attgcagaaa acgctgtcgg
     4381 aaattatacg gagtgtataa ctgcggctaa gttggcaatt gaaaagtgtg gtgcgaaaag
     4441 tgtattgctg tcctttgaac caatgaatga tttgatgagc caaaaaaatg taatcaatat
     4501 tgttgatgat aatattaaga agtaccacat ggaatatacc taatagattt cgagttgcag
     4561 cgaggcggca agtgaacgaa tccccaggag catagataac tatgtgactg gggtgagtga
     4621 aagcagccaa caaagcagca gcttgaaaga tgaagggtat aaaagagtat gacagcagtg
     4681 ctgccatact ttctaatatt atcttgagga gtaaaacagg tatgacttca tatgttgata
     4741 aacaagaaat tacagcaagc tcagaaattg atgatttgat tttttcgagc gatccattag
     4801 tgtggtctta cgacgagcag gaaaaaatca gaaagaaact tgtgcttgat gcatttcgta
     4861 atcattataa acattgtcga gaatatcgtc actactgtca ggcacacaaa gtagatgaca
     4921 atattacgga aattgatgac atacctgtat tcccaacatc ggtttttaag tttactcgct
     4981 tattaacttc tcaggaaaac gagattgaaa gttggtttac cagtagcggc acgaatggtt
     5041 taaaaagtca ggtggcgcgt gacagattaa gtattgagag actcttaggc tctgtgagtt
     5101 atggcatgaa atatgttggt agttggtttg atcatcaaat agaattagtc aatttgggac
     5161 cagatagatt taatgctcat aatatttggt ttaaatatgt tatgagtttg gtggaattgt
     5221 tatatcctac gacatttacc gtaacagaag aacgaataga ttttgttaaa acattgaata
     5281 gtcttgaacg aataaaaaat caagggaaag atctttgtct tattggttcg ccatacttta
     5341 tttatttact ctgccattat atgaaagata aaaaaatctc attttctgga gataaaagcc
     5401 tttatatcat aaccggaggc ggctggaaaa gttacgaaaa agaatctctg aaacgtgatg
     5461 atttcaatca tcttttattt gatactttca atctcagtga tattagtcag atccgagata
     5521 tatttaatca agttgaactc aacacttgtt tctttgagga tgaaatgcag cgtaaacatg
     5581 ttccgccgtg ggtatatgcg cgagcgcttg atcctgaaac gttgaaacct gtacctgatg
     5641 gaacgccggg gttgatgagt tatatggatg cgtcagcaac cagttatcca gcatttattg
     5701 ttaccgatga tgtcgggata attagcagag aatatggtaa gtatcccggc gtgctcgttg
     5761 aaattttacg tcgcgtcaat acgaggacgc agaaagggtg tgctttaagc ttaaccgaag
     5821 cgtttgatag ttgagcggcc gctctagagg catcaaataa aacgaaaggc tcagtcgaaa
     5881 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     5941 ccgccgccct agacctaggg tacgggtttt gctgcccgca aacgggctgt tctggtgttg
     6001 ctagtttgtt atcagaatcg cagatccggc ttcaggtttg ccggctgaaa gcgctatttc
     6061 ttccagaatt gccatgattt tttccccacg ggaggcgtca ctggctcccg tgttgtcggc
     6121 agctttgatt cgataagcag catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct
     6181 gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca
     6241 cctgttctat taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga
     6301 acagctttaa atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg
     6361 ttttcatctg tgcatatgga cagttttccc tttgatatct aacggtgaac agttgttcta
     6421 cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat
     6481 ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga
     6541 gaacgaacca ttgagatcat gcttactttg catgtcactc aaaaattttg cctcaaaact
     6601 ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttacgtag
     6661 gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt
     6721 ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc
     6781 agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt
     6841 acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag
     6901 cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt
     6961 ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga
     7021 cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat
     7081 ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg
     7141 gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc
     7201 tctggttgct ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc
     7261 gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg
     7321 gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg
     7381 actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc
     7441 taggtgattt taatcactat accaattgag atgggctagt caatgataat tactagtcct
     7501 tttcccggga gatctgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa
     7561 ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca
     7621 agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag
     7681 ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac
     7741 gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc
     7801 aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct
     7861 gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat
     7921 ggcactacag gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc
     7981 ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt
     8041 ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt
     8101 gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta
     8161 cccgtcttac tgtccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     8221 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     8281 ccaagcgagc tcgtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8341 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8401 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8461 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8521 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8581 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8641 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8701 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8761 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8821 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8881 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8941 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9001 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9061 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9301 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     9361 gccctttcgt cttcac
//
