LOCUS       40924_20656        7981 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU36, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 7981)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 7981)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7981)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz 
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 
            Niedersachsen 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      36..42
                     /gene="luxC"
                     /regulatory_class="ribosome_binding_site"
     CDS             48..1487
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             1503..2423
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             2475..3554
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             3572..4552
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             4734..5843
                     /label=LuxE
                     /note="LuxE"
     terminator      5866..5952
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(6116..6704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(6792..6886)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6919..7776)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7777..7881)
                     /label=AmpR promoter
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacaggag gatacgtatg actaaaaaaa
       61 tttcattcat tattaacggc caggttgaaa tctttcccga aggtgatgat ttagtgcaat
      121 ccattaattt tggtgataat agtgtttacc tgccaatatt gaatgactct catgtaaaaa
      181 acattattga ttgtaatgga aataacgaat tacggttgca taacattgtc aattttctct
      241 atacggtagg gcaaagatgg aaaaatgaag aatactcaag acgcaggaca tacattcgtg
      301 acttaaaaaa atatatggga tattcagaag aaatggctaa gctagaggcc aattggatat
      361 ctatgatttt atgttctaaa ggcggccttt atgatgttgt agaaaatgaa cttggttctc
      421 gccatatcat ggatgaatgg ctacctcagg atgaaagtta tgttcgggct tttccgaaag
      481 gtaaatctgt acatctgttg gcaggtaatg ttccattatc tgggatcatg tctatattac
      541 gcgcaatttt aactaagaat cagtgtatta taaaaacatc gtcaaccgat ccttttaccg
      601 ctaatgcatt agcgttaagt tttattgatg tagaccctaa tcatccgata acgcgctctt
      661 tatctgttat atattggccc caccaaggtg atacatcact cgcaaaagaa attatgcaac
      721 atgcggatgt tattgtcgct tggggagggc cagatgcgat taattgggcg gtagagcatg
      781 cgccatctta tgctgatgtg attaaatttg gttctaaaaa gagtctttgc attatcgata
      841 atcctgttga tttgacgtcc gcagcgacag gtgcggctca tgatgtttgt ttttacgatc
      901 agcgagcttg tttttctgcc caaaacatat attacatggg aaatcattat gaggaattta
      961 agttagcgtt gatagaaaaa cttaatctat atgcgcatat attaccgaat gccaaaaaag
     1021 attttgatga aaaggcggcc tattctttag ttcaaaaaga aagcttgttt gctggattaa
     1081 aagtagaggt ggatattcat caacgttgga tgattattga gtcaaatgca ggtgtggaat
     1141 ttaatcaacc acttggcaga tgtgtgtacc ttcatcacgt cgataatatt gagcaaatat
     1201 tgccttatgt tcaaaaaaat aagacgcaaa ccatatctat ttttccttgg gagtcatcat
     1261 ttaaatatcg agatgcgtta gcattaaaag gtgcggaaag gattgtagaa gcaggaatga
     1321 ataacatatt tcgagttggt ggatctcatg acggaatgag accgttgcaa cgattagtga
     1381 catatatttc tcatgaaagg ccatctaact atacggctaa ggatgttgcg gttgaaatag
     1441 aacagactcg attcctggaa gaagataagt tccttgtatt tgtcccataa taggtaaaaa
     1501 gtatggaaaa tgaatcaaaa tataaaacca tcgaccacgt tatttgtgtt gaaggaaata
     1561 aaaaaattca tgtttgggaa acgctgccag aagaaaacag cccaaagaga aagaatgcca
     1621 ttattattgc gtctggtttt gcccgcagga tggatcattt tgctggtctg gcggaatatt
     1681 tatcgcggaa tggatttcat gtgatccgct atgattcgct tcaccacgtt ggattgagtt
     1741 cagggacaat tgatgaattt acaatgtcta taggaaagca gagcttgtta gcagtggttg
     1801 attggttaac tacacgaaaa ataaataact tcggtatgtt ggcttcaagc ttatctgcgc
     1861 ggatagctta tgcaagccta tctgaaatca atgcttcgtt tttaatcacc gcagtcggtg
     1921 ttgttaactt aagatattct cttgaaagag ctttagggtt tgattatctc agtctaccca
     1981 ttaatgaatt gccgaataat ctagattttg aaggccataa attgggtgct gaagtctttg
     2041 cgagagattg tcttgatttt ggttgggaag atttagcttc tacaattaat aacatgatgt
     2101 atcttgatat accgtttatt gcttttactg caaataacga taattgggtc aagcaagatg
     2161 aagttatcac attgttatca aatattcgta gtaatcgatg caagatatat tctttgttag
     2221 gaagttcgca tgacttgagt gaaaatttag tggtcctgcg caatttttat caatcggtta
     2281 cgaaagccgc tatcgcgatg gataatgatc atctggatat tgatgttgat attactgaac
     2341 cgtcatttga acatttaact attgcgacag tcaatgaacg ccgaatgaga attgagattg
     2401 aaaatcaagc aatttctctg tcttaaaatc tattgagata ttctatcact caaatagcaa
     2461 tataaggact ctctatgaaa tttggaaact ttttgcttac ataccaacct ccccaatttt
     2521 ctcaaacaga ggtaatgaaa cgtttggtta aattaggtcg catctctgag gagtgtggtt
     2581 ttgataccgt atggttactg gagcatcatt tcacggagtt tggtttgctt ggtaaccctt
     2641 atgtcgctgc tgcatattta cttggcgcga ctaaaaaatt gaatgtagga actgccgcta
     2701 ttgttcttcc cacagcccat ccagtacgcc aacttgaaga tgtgaattta ttggatcaaa
     2761 tgtcaaaagg acgatttcgg tttggtattt gccgagggct ttacaacaag gactttcgcg
     2821 tattcggcac agatatgaat aacagtcgcg ccttagcgga atgctggtac gggctgataa
     2881 agaatggcat gacagaggga tatatggaag ctgataatga acatatcaag ttccataagg
     2941 taaaagtaaa ccccgcggcg tatagcagag gtggcgcacc ggtttatgtg gtggctgaat
     3001 cagcttcgac gactgagtgg gctgctcaat ttggcctacc gatgatatta agttggatta
     3061 taaatactaa cgaaaagaaa gcacaacttg agctttataa tgaagtggct caagaatatg
     3121 ggcacgatat tcataatatc gaccattgct tatcatatat aacatctgta gatcatgact
     3181 caattaaagc gaaagagatt tgccggaaat ttctggggca ttggtatgat tcttatgtga
     3241 atgctacgac tatttttgat gattcagacc aaacaagagg ttatgatttc aataaagggc
     3301 agtggcgtga ctttgtatta aaaggacata aagatactaa tcgccgtatt gattacagtt
     3361 acgaaatcaa tcccgtggga acgccgcagg aatgtattga cataattcaa aaagacattg
     3421 atgctacagg aatatcaaat atttgttgtg gatttgaagc taatggaaca gtagacgaaa
     3481 ttattgcttc catgaagctc ttccagtctg atgtcatgcc atttcttaaa gaaaaacaac
     3541 gttcgctatt atattagcta aggagaaaga aatgaaattt ggattgttct tccttaactt
     3601 catcaattca acaactgttc aagaacaaag tatagttcgc atgcaggaaa taacggagta
     3661 tgttgataag ttgaattttg aacagatttt agtgtatgaa aatcattttt cagataatgg
     3721 tgttgtcggc gctcctctga ctgtttctgg ttttctgctc ggtttaacag agaaaattaa
     3781 aattggttca ttaaatcaca tcattacaac tcatcatcct gtccgcatag cggaggaagc
     3841 ttgcttattg gatcagttaa gtgaagggag atttatttta gggtttagtg attgcgaaaa
     3901 aaaagatgaa atgcattttt ttaatcgccc ggttgaatat caacagcaac tatttgaaga
     3961 gtgttatgaa atcattaacg atgctttaac aacaggctat tgtaatccag ataacgattt
     4021 ttatagcttc cctaaaatat ctgtaaatcc ccatgcttat acgccaggcg gacctcggaa
     4081 atatgtaaca gcaaccagtc atcatattgt tgagtgggcg gccaaaaaag gtattcctct
     4141 catctttaag tgggatgatt ctaatgatgt tagatatgaa tatgctgaaa gatataaagc
     4201 cgttgcggat aaatatgacg ttgacctatc agagatagac catcagttaa tgatattagt
     4261 taactataac gaagatagta ataaagctaa acaagagacg cgtgcattta ttagtgatta
     4321 tgttcttgaa atgcacccta atgaaaattt cgaaaataaa cttgaagaaa taattgcaga
     4381 aaacgctgtc ggaaattata cggagtgtat aactgcggct aagttggcaa ttgaaaagtg
     4441 tggtgcgaaa agtgtattgc tgtcctttga accaatgaat gatttgatga gccaaaaaaa
     4501 tgtaatcaat attgttgatg ataatattaa gaagtaccac atggaatata cctaatagat
     4561 ttcgagttgc agcgaggcgg caagtgaacg aatccccagg agcatagata actatgtgac
     4621 tggggtgagt gaaagcagcc aacaaagcag cagcttgaaa gatgaagggt ataaaagagt
     4681 atgacagcag tgctgccata ctttctaata ttatcttgag gagtaaaaca ggtatgactt
     4741 catatgttga taaacaagaa attacagcaa gctcagaaat tgatgatttg attttttcga
     4801 gcgatccatt agtgtggtct tacgacgagc aggaaaaaat cagaaagaaa cttgtgcttg
     4861 atgcatttcg taatcattat aaacattgtc gagaatatcg tcactactgt caggcacaca
     4921 aagtagatga caatattacg gaaattgatg acatacctgt attcccaaca tcggttttta
     4981 agtttactcg cttattaact tctcaggaaa acgagattga aagttggttt accagtagcg
     5041 gcacgaatgg tttaaaaagt caggtggcgc gtgacagatt aagtattgag agactcttag
     5101 gctctgtgag ttatggcatg aaatatgttg gtagttggtt tgatcatcaa atagaattag
     5161 tcaatttggg accagataga tttaatgctc ataatatttg gtttaaatat gttatgagtt
     5221 tggtggaatt gttatatcct acgacattta ccgtaacaga agaacgaata gattttgtta
     5281 aaacattgaa tagtcttgaa cgaataaaaa atcaagggaa agatctttgt cttattggtt
     5341 cgccatactt tatttattta ctctgccatt atatgaaaga taaaaaaatc tcattttctg
     5401 gagataaaag cctttatatc ataaccggag gcggctggaa aagttacgaa aaagaatctc
     5461 tgaaacgtga tgatttcaat catcttttat ttgatacttt caatctcagt gatattagtc
     5521 agatccgaga tatatttaat caagttgaac tcaacacttg tttctttgag gatgaaatgc
     5581 agcgtaaaca tgttccgccg tgggtatatg cgcgagcgct tgatcctgaa acgttgaaac
     5641 ctgtacctga tggaacgccg gggttgatga gttatatgga tgcgtcagca accagttatc
     5701 cagcatttat tgttaccgat gatgtcggga taattagcag agaatatggt aagtatcccg
     5761 gcgtgctcgt tgaaatttta cgtcgcgtca atacgaggac gcagaaaggg tgtgctttaa
     5821 gcttaaccga agcgtttgat agttgagcgg ccgctctaga ggcatcaaat aaaacgaaag
     5881 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     5941 agtaggacaa atccgccgcc ctagacctag gcgttcggct gcggcgagcg gtatcagctc
     6001 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6301 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6541 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6781 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     6841 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgta
     6901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7021 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7201 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7261 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7321 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7381 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7441 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7501 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7561 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7621 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7681 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7741 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7801 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7861 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     7921 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     7981 c
//
