LOCUS       40924_20651        7969 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU35, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 7969)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 7969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz 
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 
            Niedersachsen 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             36..1475
                     /codon_start=1
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1491..2411
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2463..3542
                     /codon_start=1
                     /label=LuxA
                     /note="LuxA luciferase subunit"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3560..4540
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4722..5831
                     /codon_start=1
                     /label=LuxE
                     /note="LuxE"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      5854..5940
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(6104..6692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(6780..6874)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6907..7764)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7765..7869)
                     /label=AmpR promoter
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacatgac taaaaaaatt tcattcatta
       61 ttaacggcca ggttgaaatc tttcccgaag gtgatgattt agtgcaatcc attaattttg
      121 gtgataatag tgtttacctg ccaatattga atgactctca tgtaaaaaac attattgatt
      181 gtaatggaaa taacgaatta cggttgcata acattgtcaa ttttctctat acggtagggc
      241 aaagatggaa aaatgaagaa tactcaagac gcaggacata cattcgtgac ttaaaaaaat
      301 atatgggata ttcagaagaa atggctaagc tagaggccaa ttggatatct atgattttat
      361 gttctaaagg cggcctttat gatgttgtag aaaatgaact tggttctcgc catatcatgg
      421 atgaatggct acctcaggat gaaagttatg ttcgggcttt tccgaaaggt aaatctgtac
      481 atctgttggc aggtaatgtt ccattatctg ggatcatgtc tatattacgc gcaattttaa
      541 ctaagaatca gtgtattata aaaacatcgt caaccgatcc ttttaccgct aatgcattag
      601 cgttaagttt tattgatgta gaccctaatc atccgataac gcgctcttta tctgttatat
      661 attggcccca ccaaggtgat acatcactcg caaaagaaat tatgcaacat gcggatgtta
      721 ttgtcgcttg gggagggcca gatgcgatta attgggcggt agagcatgcg ccatcttatg
      781 ctgatgtgat taaatttggt tctaaaaaga gtctttgcat tatcgataat cctgttgatt
      841 tgacgtccgc agcgacaggt gcggctcatg atgtttgttt ttacgatcag cgagcttgtt
      901 tttctgccca aaacatatat tacatgggaa atcattatga ggaatttaag ttagcgttga
      961 tagaaaaact taatctatat gcgcatatat taccgaatgc caaaaaagat tttgatgaaa
     1021 aggcggccta ttctttagtt caaaaagaaa gcttgtttgc tggattaaaa gtagaggtgg
     1081 atattcatca acgttggatg attattgagt caaatgcagg tgtggaattt aatcaaccac
     1141 ttggcagatg tgtgtacctt catcacgtcg ataatattga gcaaatattg ccttatgttc
     1201 aaaaaaataa gacgcaaacc atatctattt ttccttggga gtcatcattt aaatatcgag
     1261 atgcgttagc attaaaaggt gcggaaagga ttgtagaagc aggaatgaat aacatatttc
     1321 gagttggtgg atctcatgac ggaatgagac cgttgcaacg attagtgaca tatatttctc
     1381 atgaaaggcc atctaactat acggctaagg atgttgcggt tgaaatagaa cagactcgat
     1441 tcctggaaga agataagttc cttgtatttg tcccataata ggtaaaaagt atggaaaatg
     1501 aatcaaaata taaaaccatc gaccacgtta tttgtgttga aggaaataaa aaaattcatg
     1561 tttgggaaac gctgccagaa gaaaacagcc caaagagaaa gaatgccatt attattgcgt
     1621 ctggttttgc ccgcaggatg gatcattttg ctggtctggc ggaatattta tcgcggaatg
     1681 gatttcatgt gatccgctat gattcgcttc accacgttgg attgagttca gggacaattg
     1741 atgaatttac aatgtctata ggaaagcaga gcttgttagc agtggttgat tggttaacta
     1801 cacgaaaaat aaataacttc ggtatgttgg cttcaagctt atctgcgcgg atagcttatg
     1861 caagcctatc tgaaatcaat gcttcgtttt taatcaccgc agtcggtgtt gttaacttaa
     1921 gatattctct tgaaagagct ttagggtttg attatctcag tctacccatt aatgaattgc
     1981 cgaataatct agattttgaa ggccataaat tgggtgctga agtctttgcg agagattgtc
     2041 ttgattttgg ttgggaagat ttagcttcta caattaataa catgatgtat cttgatatac
     2101 cgtttattgc ttttactgca aataacgata attgggtcaa gcaagatgaa gttatcacat
     2161 tgttatcaaa tattcgtagt aatcgatgca agatatattc tttgttagga agttcgcatg
     2221 acttgagtga aaatttagtg gtcctgcgca atttttatca atcggttacg aaagccgcta
     2281 tcgcgatgga taatgatcat ctggatattg atgttgatat tactgaaccg tcatttgaac
     2341 atttaactat tgcgacagtc aatgaacgcc gaatgagaat tgagattgaa aatcaagcaa
     2401 tttctctgtc ttaaaatcta ttgagatatt ctatcactca aatagcaata taaggactct
     2461 ctatgaaatt tggaaacttt ttgcttacat accaacctcc ccaattttct caaacagagg
     2521 taatgaaacg tttggttaaa ttaggtcgca tctctgagga gtgtggtttt gataccgtat
     2581 ggttactgga gcatcatttc acggagtttg gtttgcttgg taacccttat gtcgctgctg
     2641 catatttact tggcgcgact aaaaaattga atgtaggaac tgccgctatt gttcttccca
     2701 cagcccatcc agtacgccaa cttgaagatg tgaatttatt ggatcaaatg tcaaaaggac
     2761 gatttcggtt tggtatttgc cgagggcttt acaacaagga ctttcgcgta ttcggcacag
     2821 atatgaataa cagtcgcgcc ttagcggaat gctggtacgg gctgataaag aatggcatga
     2881 cagagggata tatggaagct gataatgaac atatcaagtt ccataaggta aaagtaaacc
     2941 ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt ggctgaatca gcttcgacga
     3001 ctgagtgggc tgctcaattt ggcctaccga tgatattaag ttggattata aatactaacg
     3061 aaaagaaagc acaacttgag ctttataatg aagtggctca agaatatggg cacgatattc
     3121 ataatatcga ccattgctta tcatatataa catctgtaga tcatgactca attaaagcga
     3181 aagagatttg ccggaaattt ctggggcatt ggtatgattc ttatgtgaat gctacgacta
     3241 tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag tggcgtgact
     3301 ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagttac gaaatcaatc
     3361 ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa agacattgat gctacaggaa
     3421 tatcaaatat ttgttgtgga tttgaagcta atggaacagt agacgaaatt attgcttcca
     3481 tgaagctctt ccagtctgat gtcatgccat ttcttaaaga aaaacaacgt tcgctattat
     3541 attagctaag gagaaagaaa tgaaatttgg attgttcttc cttaacttca tcaattcaac
     3601 aactgttcaa gaacaaagta tagttcgcat gcaggaaata acggagtatg ttgataagtt
     3661 gaattttgaa cagattttag tgtatgaaaa tcatttttca gataatggtg ttgtcggcgc
     3721 tcctctgact gtttctggtt ttctgctcgg tttaacagag aaaattaaaa ttggttcatt
     3781 aaatcacatc attacaactc atcatcctgt ccgcatagcg gaggaagctt gcttattgga
     3841 tcagttaagt gaagggagat ttattttagg gtttagtgat tgcgaaaaaa aagatgaaat
     3901 gcattttttt aatcgcccgg ttgaatatca acagcaacta tttgaagagt gttatgaaat
     3961 cattaacgat gctttaacaa caggctattg taatccagat aacgattttt atagcttccc
     4021 taaaatatct gtaaatcccc atgcttatac gccaggcgga cctcggaaat atgtaacagc
     4081 aaccagtcat catattgttg agtgggcggc caaaaaaggt attcctctca tctttaagtg
     4141 ggatgattct aatgatgtta gatatgaata tgctgaaaga tataaagccg ttgcggataa
     4201 atatgacgtt gacctatcag agatagacca tcagttaatg atattagtta actataacga
     4261 agatagtaat aaagctaaac aagagacgcg tgcatttatt agtgattatg ttcttgaaat
     4321 gcaccctaat gaaaatttcg aaaataaact tgaagaaata attgcagaaa acgctgtcgg
     4381 aaattatacg gagtgtataa ctgcggctaa gttggcaatt gaaaagtgtg gtgcgaaaag
     4441 tgtattgctg tcctttgaac caatgaatga tttgatgagc caaaaaaatg taatcaatat
     4501 tgttgatgat aatattaaga agtaccacat ggaatatacc taatagattt cgagttgcag
     4561 cgaggcggca agtgaacgaa tccccaggag catagataac tatgtgactg gggtgagtga
     4621 aagcagccaa caaagcagca gcttgaaaga tgaagggtat aaaagagtat gacagcagtg
     4681 ctgccatact ttctaatatt atcttgagga gtaaaacagg tatgacttca tatgttgata
     4741 aacaagaaat tacagcaagc tcagaaattg atgatttgat tttttcgagc gatccattag
     4801 tgtggtctta cgacgagcag gaaaaaatca gaaagaaact tgtgcttgat gcatttcgta
     4861 atcattataa acattgtcga gaatatcgtc actactgtca ggcacacaaa gtagatgaca
     4921 atattacgga aattgatgac atacctgtat tcccaacatc ggtttttaag tttactcgct
     4981 tattaacttc tcaggaaaac gagattgaaa gttggtttac cagtagcggc acgaatggtt
     5041 taaaaagtca ggtggcgcgt gacagattaa gtattgagag actcttaggc tctgtgagtt
     5101 atggcatgaa atatgttggt agttggtttg atcatcaaat agaattagtc aatttgggac
     5161 cagatagatt taatgctcat aatatttggt ttaaatatgt tatgagtttg gtggaattgt
     5221 tatatcctac gacatttacc gtaacagaag aacgaataga ttttgttaaa acattgaata
     5281 gtcttgaacg aataaaaaat caagggaaag atctttgtct tattggttcg ccatacttta
     5341 tttatttact ctgccattat atgaaagata aaaaaatctc attttctgga gataaaagcc
     5401 tttatatcat aaccggaggc ggctggaaaa gttacgaaaa agaatctctg aaacgtgatg
     5461 atttcaatca tcttttattt gatactttca atctcagtga tattagtcag atccgagata
     5521 tatttaatca agttgaactc aacacttgtt tctttgagga tgaaatgcag cgtaaacatg
     5581 ttccgccgtg ggtatatgcg cgagcgcttg atcctgaaac gttgaaacct gtacctgatg
     5641 gaacgccggg gttgatgagt tatatggatg cgtcagcaac cagttatcca gcatttattg
     5701 ttaccgatga tgtcgggata attagcagag aatatggtaa gtatcccggc gtgctcgttg
     5761 aaattttacg tcgcgtcaat acgaggacgc agaaagggtg tgctttaagc ttaaccgaag
     5821 cgtttgatag ttgagcggcc gctctagagg catcaaataa aacgaaaggc tcagtcgaaa
     5881 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     5941 ccgccgccct agacctaggc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     6301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6541 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgact agtgcttgga
     6781 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     6841 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgtaaa cttggtctga
     6901 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6961 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7021 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7081 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7141 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7201 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7261 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7321 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7381 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7441 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7501 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7561 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7621 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7681 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7741 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7801 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7861 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     7921 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
