LOCUS       40924_20646        3197 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU168, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 3197)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3197)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz 
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 
            Niedersachsen 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      37..42
                     /gene="gfpmut3.1"
                     /regulatory_class="ribosome_binding_site"
     CDS             51..764
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      787..873
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1037..1625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1713..1807)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1853..3040)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(3088..3116)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ctcgaggatc ccgggtacct gcagctagcg tcgacgagga gaaattaagc atgcgtaaag
       61 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      121 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      181 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      241 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      301 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      361 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      421 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      481 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      541 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      601 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      661 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      721 taacagctgc tgggattaca catggcatgg atgaactata caaataagcg gccgctctag
      781 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      841 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta ggcgttcggc
      901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1201 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     1261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1441 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1621 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1681 gttaagggat tttggtcatg actagtgctt ggattctcac caataaaaaa cgcccggcgg
     1741 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     1801 aggagtccaa gcgagctcaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
     1861 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc
     1921 ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga
     1981 tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc
     2041 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
     2101 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
     2161 gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga
     2221 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
     2281 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
     2341 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
     2401 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
     2461 agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     2521 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     2581 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     2641 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     2701 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc cacaggacgg
     2761 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
     2821 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
     2881 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
     2941 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
     3001 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
     3061 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
     3121 gagacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3181 ggccctttcg tcttcac
//
