LOCUS       40924_20586       12211 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFTarget2-Tn7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Kvitko BH, McMillan IA, Schweizer HP.
  TITLE     An improved method for the oriT-directed cloning and 
            functionalization of large bacterial genomic regions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   Kvitko BH, McMillan IA, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2012) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 12211)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12211)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-SEP-2012) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..372
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     protein_bind    complement(486..533)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(586..672)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(775..869)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(897..913)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   918..1116
                     /label=Tn7R
                     /note="Tn7R"
     misc_feature    1264..1281
                     /label=I-SceI restriction site
                     /note="I-SceI restriction site"
     primer_bind     complement(1291..1307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1657..1743
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1835..1862
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     oriT            2309..2417
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2627..3418)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3509..5317)
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVAHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             5675..6022
                     /codon_start=1
                     /gene="redF'"
                     /product="RedF'"
                     /label=redF'
                     /protein_id="AGO61965.1"
                     /translation="MERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVP
                     VTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPE
                     SDAVAMLKQLS"
     gene            5675..6022
                     /gene="redF'"
                     /label=redF'
     rep_origin      6417..7031
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      7107..7326
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             7417..8169
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    8175..8425
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             8751..9923
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             9926..10894
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    10970..11443
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    11698..11715
                     /label=I-SceI restriction site
                     /note="I-SceI restriction site"
     mobile_element  11716..11881
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    11893..11917
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    11918..11922
                     /label=target insert site
                     /note="target insert site"
     protein_bind    complement(11923..11947)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12009..12056)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        12146..12193
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
ORIGIN
        1 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
       61 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
      121 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
      181 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
      241 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
      301 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
      361 gaggagcagg actgatcatg atgatatatt tttatcttgt gcaatgtaac atcagagata
      421 agccgaatta tctagaatta ttccattgag taagttttta agcacatcag cttcaaaagc
      481 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggtac ttcaagatcc
      541 ccaattcgag ctcgattcgt ccgggccgca agctcctagc ggcggatttg tcctactcag
      601 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
      661 tcgttttatt tgatgcctca agctagagag tcattacccc aggcgtttaa gggcaccaat
      721 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtctagc ttggattctc
      781 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
      841 ggtcattact ggatctatca acaggagtcc aagctcagct aattaagcta gcttatcgat
      901 accgtcgacc tcgaacccca cgcccctctt taatacgacg ggcaatttgc acttcagaaa
      961 atgaagagtt tgctttagcc ataacaaaag tccagtatgc tttttcacag cataactgga
     1021 ctgatttcag tttacaacta ttctgtctag tttaagactt tattgtcata gtttagatct
     1081 attttgttca gtttaagact ttattgtccg cccacacccg cttacgcagg gcatccattt
     1141 attactcaac cgtaaccgat tttgccaggt tacgcggctg gtcctctagc tggcgtaata
     1201 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     1261 gggtagggat aacagggtaa tgagcttggc actggccgtc gttttacaac gtcgtgactg
     1321 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1381 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1441 cgaatggcga gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
     1501 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
     1561 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
     1621 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     1681 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     1741 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
     1801 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
     1861 tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata tgcatgcgcc
     1921 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tcgacatccg
     1981 ccctcaccgc caggaacgca accgcagcct catcacgccg gcgcttcttg gccgcgcggg
     2041 attcaaccca ctcggccagc tcgtcggtgt agctctttgg catcgtctct cgcctgtccc
     2101 ctcagttcag taatttcctg catttgcctg tttccagtcg gtagatattc cacaaaacag
     2161 cagggaagca gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
     2221 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
     2281 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
     2341 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
     2401 ctctgcgagg ctggccgata agcttggcgt aatcatggtc atcatatgga attctgggcg
     2461 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg
     2521 attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg
     2581 ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa
     2641 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga
     2701 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt
     2761 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat
     2821 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt
     2881 cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt
     2941 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc
     3001 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca
     3061 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct
     3121 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca
     3181 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca
     3241 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
     3301 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
     3361 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     3421 gaaacgatcc tcatcctgtc tcttgatcag atccattccc caccgaggct gtagccgacg
     3481 atggtgcgcc aggagagttg ttgattcatt gtttgcctcc ctgctgcggt ttttcaccga
     3541 agttcatgcc agtccagcgt ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc
     3601 gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat
     3661 cggcgaaatt ccatacctgt tcaccgacga cggcgctgac gcgatcaaag acgcggtgat
     3721 acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac attgagtgca
     3781 gcccggctaa cgtatccacg ccgtattcgg tgatgataat cggctgatgc agtttctcct
     3841 gccaggccag aagttctttt tccagtacct tctctgccgt ttccaaatcg ccgctttgga
     3901 cataccatcc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg
     3961 tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcggg tcgagtttac
     4021 gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt
     4081 cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgct atcagctctt
     4141 taatcgcctg taagtgcgct tgctgagttt ccccgttgac tgcctcttcg ctgtacagtt
     4201 ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagcag
     4261 cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc tcttcagcgt
     4321 aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattaat gcgtggtcgt
     4381 gcaccatcag cacgttatcg aatcctttgc cacgcaagtc cgcatcttca tgacgaccaa
     4441 agccagtaaa gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc actgccactg
     4501 accggatgcc gacgcgaagc gggtagatat cacactctgt ctggcttttg gctgtgacgc
     4561 acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc acttgcaaag
     4621 tcccgctagt gccttgtcca gttgcaacca cctgttgatc cgcatcacgc agttcaacgc
     4681 tgacatcacc attggccacc acctgccagt caacagacgc gtggttacag tcttgcgcga
     4741 catgcgccac cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc
     4801 tgcgatggat tccggcatag ttaaagaaat catggaagta agactgcttt ttcttgccgt
     4861 tttcgtcggt aatcaccatt cccggcggga tagtctgcca gttcagttcg ttgttcacac
     4921 aaacggtgat acgtacactt ttcccggcaa taacatacgg cgtgacatcg gcttcaaatg
     4981 gcgtatagcc gccctgatgc tccatcactt cctgattatt gacccacact ttgccgtaat
     5041 gagtgaccgc atcgaaacgc agcacgatac gttggcctgc ccaacctttc ggtataaaga
     5101 cttcgcgctg ataccagacg ttgcccgcat aattacgaat atctgcatcg gcgaactgat
     5161 cgttaaaact gcctggcaca gcaattgccc ggctttcttg taacgcgctt tcccaccaac
     5221 gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacaggccg tcgagttttt
     5281 tgatttcacg ggttggggtt tctacaggac ggaccatggt cgactcctcc ttagctggat
     5341 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     5401 ggctccccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     5461 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     5521 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     5581 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
     5641 tgcttgcggc agcgtgaatt cgagcgataa gctcatggag cggcgtaacc gtcgcacagg
     5701 aaggacagag aaagcgcgga tctgggaagt gacggacaga acggtcagga cctggattgg
     5761 ggaggcggtt gccgccgctg ctgctgacgg tgtgacgttc tctgttccgg tcacaccaca
     5821 tacgttccgc cattcctatg cgatgcacat gctgtatgcc ggtataccgc tgaaagttct
     5881 gcaaagcctg atgggacata agtccatcag ttcaacggaa gtctacacga aggtttttgc
     5941 gctggatgtg gctgcccggc accgggtgca gtttgcgatg ccggagtctg atgcggttgc
     6001 gatgctgaaa caattatcct gagaataaat gccttggcct ttatatggaa atgtggaact
     6061 gagtggatat gctgtttttg tctgttaaac agagaagctg gctgttatcc actgagaagc
     6121 gaacgaaaca gtcgggaaaa tctcccatta tcgtagagat ccgcattatt aatctcagga
     6181 gcctgtgtag cgtttatagg aagtagtgtt ctgtcatgat gcctgcaagc ggtaacgaaa
     6241 acgatttgaa tatgccttca ggaacaatag aaatcttcgt gcggtgttac gttgaagtgg
     6301 agcggattat gtcagcaatg gacagaacaa cctaatgaac acagaaccat gatgtggtct
     6361 gtccttttac agccagtagt gctcgccgca gtcgagcgac agggcgaagc cctcggctgg
     6421 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt
     6481 cgaagccgtg tgcgagacac cgcggccggc cgccggcgtt gtggatacct cgcggaaaac
     6541 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
     6601 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
     6661 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
     6721 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
     6781 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
     6841 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
     6901 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
     6961 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
     7021 cgaaccctcc cggtcgagtg agcgaggaag caccagggaa cagcacttat atattctgct
     7081 tacacacgat gcctgaaaaa acttcccttg gggttatcca cttatccacg gggatatttt
     7141 tataattatt ttttttatag tttttagatc ttctttttta gagcgccttg taggccttta
     7201 tccatgctgg ttctagagaa ggtgttgtga caaattgccc tttcagtgtg acaaatcacc
     7261 ctcaaatgac agtcctgtct gtgacaaatt gcccttaacc ctgtgacaaa ttgccctcag
     7321 aagaagctgt tttttcacaa agttatccct gcttattgac tcttttttat ttagtgtgac
     7381 aatctaaaaa cttgtcacac ttcacatgga tctgtcatgg cggaaacagc ggttatcaat
     7441 cacaagaaac gtaaaaatag cccgcgaatc gtccagtcaa acgacctcac tgaggcggca
     7501 tatagtctct cccgggatca aaaacgtatg ctgtatctgt tcgttgacca gatcagaaaa
     7561 tctgatggca ccctacagga acatgacggt atctgcgaga tccatgttgc taaatatgct
     7621 gaaatattcg gattgacctc tgcggaagcc agtaaggata tacggcaggc attgaagagt
     7681 ttcgcgggga aggaagtggt tttttatcgc cctgaagagg atgccggcga tgaaaaaggc
     7741 tatgaatctt ttccttggtt tatcaaacgt gcgcacagtc catccagagg gctttacagt
     7801 gtacatatca acccatatct cattcccttc tttatcgggt tacagaaccg gtttacgcag
     7861 tttcggctta gtgaaacaaa agaaatcacc aatccgtatg ccatgcgttt atacgaatcc
     7921 ctgtgtcagt atcgtaagcc ggatggctca ggcatcgtct ctctgaaaat cgactggatc
     7981 atagagcgtt accagctgcc tcaaagttac cagcgtatgc ctgacttccg ccgccgcttc
     8041 ctgcaggtct gtgttaatga gatcaacagc agaactccaa tgcgcctctc atacattgag
     8101 aaaaagaaag gccgccagac gactcatatc gtattttcct tccgcgatat cacttccatg
     8161 acgacaggat agtctgaggg ttatctgtca cagatttgag ggtggttcgt cacatttgtt
     8221 ctgacctact gagggtaatt tgtcacagtt ttgctgtttc cttcagcctg catggatttt
     8281 ctcatacttt ttgaactgta atttttaagg aagccaaatt tgagggcagt ttgtcacagt
     8341 tgatttcctt ctctttccct tcgtcatgtg acctgatatc gggggttagt tcgtcatcat
     8401 tgatgagggt tgattatcac agtttattac tctgaattgg ctatccgcgt gtgtacctct
     8461 acctggagtt tttcccacgg tggatatttc ttcttgcgct gagcgtaaga gctatctgac
     8521 agaacagttc ttctttgctt cctcgccagt tcgctcgcta tgctcggtta cacggctgcg
     8581 gcgagcgcta gtgataataa gtgactgagg tatgtgctct tcttatctcc ttttgtagtg
     8641 ttgctcttat tttaaacaac tttgcggttt tttgatgact ttgcgatttt gttgttgctt
     8701 tgcagtaaat tgcaagattt aataaaaaaa cgcaaagcaa tgattaaagg atgttcagaa
     8761 tgaaactcat ggaaacactt aaccagtgca taaacgctgg tcatgaaatg acgaaggcta
     8821 tcgccattgc acagtttaat gatgacagcc cggaagcgag gaaaataacc cggcgctgga
     8881 gaataggtga agcagcggat ttagttgggg tttcttctca ggctatcaga gatgccgaga
     8941 aagcagggcg actaccgcac ccggatatgg aaattcgagg acgggttgag caacgtgttg
     9001 gttatacaat tgaacaaatt aatcatatgc gtgatgtgtt tggtacgcga ttgcgacgtg
     9061 ctgaagacgt atttccaccg gtgatcgggg ttgctgccca taaaggtggc gtttacaaaa
     9121 cctcagtttc tgttcatctt gctcaggatc tggctctgaa ggggctacgt gttttgctcg
     9181 tggaaggtaa cgacccccag ggaacagcct caatgtatca cggatgggta ccagatcttc
     9241 atattcatgc agaagacact ctcctgcctt tctatcttgg ggaaaaggac gatgtcactt
     9301 atgcaataaa gcccacttgc tggccggggc ttgacattat tccttcctgt ctggctctgc
     9361 accgtattga aactgagtta atgggcaaat ttgatgaagg taaactgccc accgatccac
     9421 acctgatgct ccgactggcc attgaaactg ttgctcatga ctatgatgtc atagttattg
     9481 acagcgcgcc taacctgggt atcggcacga ttaatgtcgt atgtgctgct gatgtgctga
     9541 ttgttcccac gcctgctgag ttgtttgact acacctccgc actgcagttt ttcgatatgc
     9601 ttcgtgatct gctcaagaac gttgatctta aagggttcga gcctgatgta cgtattttgc
     9661 ttaccaaata cagcaatagt aatggctctc agtccccgtg gatggaggag caaattcggg
     9721 atgcctgggg aagcatggtt ctaaaaaatg ttgtacgtga aacggatgaa gttggtaaag
     9781 gtcagatccg gatgagaact gtttttgaac aggccattga tcaacgctct tcaactggtg
     9841 cctggagaaa tgctctttct atttgggaac ctgtctgcaa tgaaattttc gatcgtctga
     9901 ttaaaccacg ctgggagatt agataatgaa gcgtgcgcct gttattccaa aacatacgct
     9961 caatactcaa ccggttgaag atacttcgtt atcgacacca gctgccccga tggtggattc
    10021 gttaattgcg cgcgtaggag taatggctcg cggtaatgcc attactttgc ctgtatgtgg
    10081 tcgggatgtg aagtttactc ttgaagtgct ccggggtgat agtgttgaga agacctctcg
    10141 ggtatggtca ggtaatgaac gtgaccagga gctgcttact gaggacgcac tggatgatct
    10201 catcccttct tttctactga ctggtcaaca gacaccggcg ttcggtcgaa gagtatctgg
    10261 tgtcatagaa attgccgatg ggagtcgccg tcgtaaagct gctgcactta ccgaaagtga
    10321 ttatcgtgtt ctggttggcg agctggatga tgagcagatg gctgcattat ccagattggg
    10381 taacgattat cgcccaacaa gtgcttatga acgtggtcag cgttatgcaa gccgattgca
    10441 gaatgaattt gctggaaata tttctgcgct ggctgatgcg gaaaatattt cacgtaagat
    10501 tattacccgc tgtatcaaca ccgccaaatt gcctaaatca gttgttgctc ttttttctca
    10561 ccccggtgaa ctatctgccc ggtcaggtga tgcacttcaa aaagccttta cagataaaga
    10621 ggaattactt aagcagcagg catctaacct tcatgagcag aaaaaagctg gggtgatatt
    10681 tgaagctgaa gaagttatca ctcttttaac ttctgtgctt aaaacgtcat ctgcatcaag
    10741 aactagttta agctcacgac atcagtttgc tcctggagcg acagtattgt ataagggcga
    10801 taaaatggtg cttaacctgg acaggtctcg tgttccaact gagtgtatag agaaaattga
    10861 ggccattctt aaggaacttg aaaagccagc accctgatgc gaccacgttt tagtctacgt
    10921 ttatctgtct ttacttaatg tcctttgtta caggccagaa agcataactg gcctgaatat
    10981 tctctctggg cccactgttc cacttgtatc gtcggtctga taatcagact gggaccacgg
    11041 tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg
    11101 gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat aatcagactg
    11161 ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccat ggtcccactc
    11221 gtatcgtcgg tctgattatt agtctgggac cacggtccca ctcgtatcgt cggtctgatt
    11281 attagtctgg aaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg
    11341 gtcccactcg tatcgtcggt ctgattatta gtctgggacc acgatcccac tcgtgttgtc
    11401 ggtctgatta tcggtctggg accacggtcc cacttgtatt gtcgatcaga ctatcagcgt
    11461 gagactacga ttccatcaat gcctgtcaag ggcaagtatt gacatgtcgt cgtaacctgt
    11521 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
    11581 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
    11641 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgctctag
    11701 ggataacagg gtaattgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
    11761 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
    11821 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
    11881 tgggtaccca tcacaagttt gtacaaaaaa gcaggctnnn nnacccagct ttcttgtaca
    11941 aagtggtgat ggggatccac tagtgagctc atgcatgatc gagctcgaat tagcttcaaa
    12001 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaga
    12061 tcccctgatt ccctttgtca acagcaatgg atttcggctt cgacgcgtaa ctcacgttaa
    12121 gggattttgg tcatgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
    12181 agtataatac gacaaggtga ggaactaaac c
//
