LOCUS       40924_20621        3570 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Trimethoprim resistance FRT vector pFTP1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP.
  TITLE     A Tn7-based broad-range bacterial cloning and expression system
  JOURNAL   Nat. Methods 2 (6), 443-448 (2005)
  PUBMED    15908923
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Choi K-H., Gaynor JB, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2004) Microbiology, Immunology and Pathology, 
            Colorado State University, Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 3570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2005"; volume: "2"; issue: "6"; pages: "443-448"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2004) Microbiology, Immunology and Pathology, Colorado State
            University, Fort Collins, CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(399..455)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     protein_bind    464..511
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(576..809)
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(1136..1164)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_recomb     1216..1263
                     /note="FRT; Flp recombinase target site"
     protein_bind    1216..1263
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1280..1336
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1349..1365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1373..1389)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1397..1427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1442..1463)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1751..2339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2513..3370)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3371..3475)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg aattggggat cttgaagtac ctattccgaa
      481 gttcctattc tctagaaagt ataggaactt cagagcgctt ttgaagctga tgtgcttaaa
      541 aacttactca atggaataat tctagataat tcttaggcca cacgttcaag tgcagccaca
      601 ggataaattt gcactgagcc tgggtgggat tcggactcga ccgcatagcc ttcaggagtg
      661 agttttgtgc aataccaacc gacgacttga ccctgccaag cggcaccaga tttcttgcgt
      721 acgcgatccc ctaagccaaa ggtggcactc aggggaagcg caaactgccc tgcaacggga
      781 gcgttggctt catcgctact ttgacccatg tcgaatcctt cttgtgaatc tattatggcg
      841 acaagcaaat cgagctctga ctgcctaccc cacaacaact actagaaagc accagcacaa
      901 cggctgccta actttgtttt agggcgacta ccctgctgcg taacatcgtt gctgttccat
      961 aacatcaaac atcgacccac ggcgtaacgc gcttagctag cttggatgcc cgaggctaga
     1021 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     1081 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     1141 acgaaccgaa caggcttatg tcaactgggt tcgtgaatta tccattgctg ttgacaaagg
     1201 gaatcagggg atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     1261 ttcagagcgc ttttgaagct aattcgagct cggtacccgg ggatcctcta gagtcgacct
     1321 gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1381 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1441 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1501 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1561 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1621 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1681 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1741 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1801 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1861 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1921 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1981 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2041 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2101 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2161 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2221 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2281 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2341 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2401 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2461 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2521 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2581 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2641 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2701 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2761 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2821 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2881 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2941 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3001 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3061 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3121 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3181 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3241 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3301 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3361 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3421 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3481 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3541 aaaataggcg tatcacgagg ccctttcgtc
//
