LOCUS       V006175                 8956 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006175
VERSION     V006175
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Geller SF, Ge PS, Visel M, Flannery JG.
  TITLE     In vitro analysis of promoter activity in Muller cells
  JOURNAL   Mol. Vis. 14, 691-705 (2008)
   PUBMED   18437242
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   Geller SF, Ge PS, Visel M, Flannery JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2009) Helen Wills Neuroscience Institute,
            University of California, Berkeley, 114 Barker Hall, Berkeley, CA
            94720, USA
REFERENCE   3  (bases 1 to 8956)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Vis.
            14, 691-705 (2008)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-MAR-2009) Helen Wills Neuroscience Institute, University of
            California, Berkeley, 114 Barker Hall, Berkeley, CA 94720, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..623
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        624..826
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             841..1021
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1074..1199
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1692..1925
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2110..2154
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2303..2344
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2452..2569
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     polyA_signal    2620..2668
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    2682..2773
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    2789..2863
                     /label="MCS - multiple cloning site"
                     /note="MCS - multiple cloning site"
     CDS             2888..3604
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    3648..4236
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4239..4255)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     repeat_region   4441..4942
                     /label="3' LTR - Lentiviral long terminal repeat"
                     /note="3' LTR - Lentiviral long terminal repeat"
     LTR             4761..4941
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4973..5197
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5243..5671
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5685..6014
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        6062..6109
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             6128..6499
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    6632..6765
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6802..6818)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6826..6842)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6850..6880)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6895..6916)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7204..7792)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7966..8823)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8824..8928)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gggcgccact agttattaat agtaatcaat tacggggtca ttagttcata
      301 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      361 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      421 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      481 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      541 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      601 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      661 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      721 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      781 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
      841 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      901 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      961 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     1021 agtactagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     1081 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     1141 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     1201 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     1261 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1321 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1381 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1441 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1501 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     1561 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1621 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1681 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1741 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1801 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1861 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1921 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1981 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     2041 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     2101 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     2161 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     2221 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     2281 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     2341 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     2401 tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag
     2461 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2521 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     2581 cagggacagc agagatccag tttggttaat cgcttcgaaa ataaaatatc tttattttca
     2641 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
     2701 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     2761 agaacatttc tctttaattc gaattaatta agcgatcgcg cttagccggt ccgatttaaa
     2821 tggcgcgccg ttaaccgtac gcctgcaggg ctagcggtta ccggatcccc gggtaccggt
     2881 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2941 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3001 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3061 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3121 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3181 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3241 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3301 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3361 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3421 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3481 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3541 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3601 caagtaaagc ggccgcgact ctagaattcg atatcaagct tatcgataat caacctctgg
     3661 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3721 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3781 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3841 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3901 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3961 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4021 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     4081 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4141 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4201 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     4261 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     4321 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     4381 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4441 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     4501 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     4561 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     4621 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     4681 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     4741 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4801 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4861 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4921 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     4981 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5041 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5101 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5161 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5221 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5281 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5341 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5401 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5461 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5521 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5581 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5641 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     5701 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5761 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5821 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5881 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5941 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6001 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     6061 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     6121 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     6181 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     6241 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     6301 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     6361 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     6421 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     6481 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     6541 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6601 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6661 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6721 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6781 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6841 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6901 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6961 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7621 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8041 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8281 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8581 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8821 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8941 aaaagtgcca cctgac
//
