LOCUS       40924_20606       11445 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFTCAPL-GW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Kvitko BH, McMillan IA, Schweizer HP.
  TITLE     An Improved Method for oriT-Directed Cloning and Functionalization 
            of Large Bacterial Genomic Regions
  JOURNAL   Appl. Environ. Microbiol. 79 (16), 4869-4878 (2013)
  PUBMED    23747708
REFERENCE   2  (bases 1 to 11445)
  AUTHORS   Kvitko BH, McMillan IA, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2012) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 11445)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11445)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2013"; volume: "79"; issue: "16"; 
            pages: "4869-4878"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-SEP-2012) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             165..1337
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             1340..2308
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    2384..2857
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    3112..3129
                     /label=I-SceI restriction site
                     /note="I-SceI restriction site"
     mobile_element  3130..3295
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    3307..3431
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3456..3486
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3540..4196
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             4541..4843
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4887..5011)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     oriT            5168..5276
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(5486..6277)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6368..8176)
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVAHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             8534..8881
                     /codon_start=1
                     /gene="redF'"
                     /product="RedF'"
                     /label=redF'
                     /protein_id="AGM33196.1"
                     /translation="MERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVP
                     VTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPE
                     SDAVAMLKQLS"
     gene            8534..8881
                     /gene="redF'"
                     /label=redF'
     rep_origin      9276..9890
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      9966..10185
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      9966..10182
                     /label=F ori2
                     /note="F ori2"
     CDS             10276..11028
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    11034..11284
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
ORIGIN
        1 gctagtgata ataagtgact gaggtatgtg ctcttcttat ctccttttgt agtgttgctc
       61 ttattttaaa caactttgcg gttttttgat gactttgcga ttttgttgtt gctttgcagt
      121 aaattgcaag atttaataaa aaaacgcaaa gcaatgatta aaggatgttc agaatgaaac
      181 tcatggaaac acttaaccag tgcataaacg ctggtcatga aatgacgaag gctatcgcca
      241 ttgcacagtt taatgatgac agcccggaag cgaggaaaat aacccggcgc tggagaatag
      301 gtgaagcagc ggatttagtt ggggtttctt ctcaggctat cagagatgcc gagaaagcag
      361 ggcgactacc gcacccggat atggaaattc gaggacgggt tgagcaacgt gttggttata
      421 caattgaaca aattaatcat atgcgtgatg tgtttggtac gcgattgcga cgtgctgaag
      481 acgtatttcc accggtgatc ggggttgctg cccataaagg tggcgtttac aaaacctcag
      541 tttctgttca tcttgctcag gatctggctc tgaaggggct acgtgttttg ctcgtggaag
      601 gtaacgaccc ccagggaaca gcctcaatgt atcacggatg ggtaccagat cttcatattc
      661 atgcagaaga cactctcctg cctttctatc ttggggaaaa ggacgatgtc acttatgcaa
      721 taaagcccac ttgctggccg gggcttgaca ttattccttc ctgtctggct ctgcaccgta
      781 ttgaaactga gttaatgggc aaatttgatg aaggtaaact gcccaccgat ccacacctga
      841 tgctccgact ggccattgaa actgttgctc atgactatga tgtcatagtt attgacagcg
      901 cgcctaacct gggtatcggc acgattaatg tcgtatgtgc tgctgatgtg ctgattgttc
      961 ccacgcctgc tgagttgttt gactacacct ccgcactgca gtttttcgat atgcttcgtg
     1021 atctgctcaa gaacgttgat cttaaagggt tcgagcctga tgtacgtatt ttgcttacca
     1081 aatacagcaa tagtaatggc tctcagtccc cgtggatgga ggagcaaatt cgggatgcct
     1141 ggggaagcat ggttctaaaa aatgttgtac gtgaaacgga tgaagttggt aaaggtcaga
     1201 tccggatgag aactgttttt gaacaggcca ttgatcaacg ctcttcaact ggtgcctgga
     1261 gaaatgctct ttctatttgg gaacctgtct gcaatgaaat tttcgatcgt ctgattaaac
     1321 cacgctggga gattagataa tgaagcgtgc gcctgttatt ccaaaacata cgctcaatac
     1381 tcaaccggtt gaagatactt cgttatcgac accagctgcc ccgatggtgg attcgttaat
     1441 tgcgcgcgta ggagtaatgg ctcgcggtaa tgccattact ttgcctgtat gtggtcggga
     1501 tgtgaagttt actcttgaag tgctccgggg tgatagtgtt gagaagacct ctcgggtatg
     1561 gtcaggtaat gaacgtgacc aggagctgct tactgaggac gcactggatg atctcatccc
     1621 ttcttttcta ctgactggtc aacagacacc ggcgttcggt cgaagagtat ctggtgtcat
     1681 agaaattgcc gatgggagtc gccgtcgtaa agctgctgca cttaccgaaa gtgattatcg
     1741 tgttctggtt ggcgagctgg atgatgagca gatggctgca ttatccagat tgggtaacga
     1801 ttatcgccca acaagtgctt atgaacgtgg tcagcgttat gcaagccgat tgcagaatga
     1861 atttgctgga aatatttctg cgctggctga tgcggaaaat atttcacgta agattattac
     1921 ccgctgtatc aacaccgcca aattgcctaa atcagttgtt gctctttttt ctcaccccgg
     1981 tgaactatct gcccggtcag gtgatgcact tcaaaaagcc tttacagata aagaggaatt
     2041 acttaagcag caggcatcta accttcatga gcagaaaaaa gctggggtga tatttgaagc
     2101 tgaagaagtt atcactcttt taacttctgt gcttaaaacg tcatctgcat caagaactag
     2161 tttaagctca cgacatcagt ttgctcctgg agcgacagta ttgtataagg gcgataaaat
     2221 ggtgcttaac ctggacaggt ctcgtgttcc aactgagtgt atagagaaaa ttgaggccat
     2281 tcttaaggaa cttgaaaagc cagcaccctg atgcgaccac gttttagtct acgtttatct
     2341 gtctttactt aatgtccttt gttacaggcc agaaagcata actggcctga atattctctc
     2401 tgggcccact gttccacttg tatcgtcggt ctgataatca gactgggacc acggtcccac
     2461 tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga
     2521 ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgataatcag actgggacca
     2581 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccatggtccc actcgtatcg
     2641 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt
     2701 ctggaaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
     2761 ctcgtatcgt cggtctgatt attagtctgg gaccacgatc ccactcgtgt tgtcggtctg
     2821 attatcggtc tgggaccacg gtcccacttg tattgtcgat cagactatca gcgtgagact
     2881 acgattccat caatgcctgt caagggcaag tattgacatg tcgtcgtaac ctgtagaacg
     2941 gagtaacctc ggtgtgcggt tgtatgcctg ctgtggattg ctgctgtgtc ctgcttatcc
     3001 acaacatttt gcgcacggtt atgtggacaa aatacctggt tacccaggcc gtgccggcac
     3061 gttaaccggg ctgcatccga tgcaagtgtg tcgctgtcga cgagctcgct ctagggataa
     3121 cagggtaatt gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     3181 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     3241 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttgggta
     3301 cccatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     3361 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     3421 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     3481 ataatgtgtg gattttgagt tagggtccgg cgagattttc aggagctaag gaagctaaaa
     3541 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     3601 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     3661 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     3721 acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg
     3781 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     3841 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     3901 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     3961 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     4021 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     4081 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
     4141 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     4201 cgcgtgggtc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     4261 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat
     4321 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     4381 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     4441 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     4501 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     4561 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     4621 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     4681 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     4741 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     4801 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     4861 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     4921 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     4981 tttctcgttc agctttcttg tacaaagtgg tgatggggat ccactagtgt atactttccg
     5041 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     5101 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     5161 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     5221 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgataa
     5281 gcttggcgta atcatggtca tcatatggaa ttctgggcga agaactccag catgagatcc
     5341 ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca
     5401 tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc
     5461 atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     5521 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     5581 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     5641 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     5701 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc
     5761 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     5821 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     5881 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     5941 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     6001 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     6061 tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca
     6121 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     6181 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     6241 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     6301 cttgatcaga tccattcccc accgaggctg tagccgacga tggtgcgcca ggagagttgt
     6361 tgattcattg tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca gtccagcgtt
     6421 tttgcagcag aaaagccgcc gacttcggtt tgcggtcgcg agtgaagatc cctttcttgt
     6481 taccgccaac gcgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc catacctgtt
     6541 caccgacgac ggcgctgacg cgatcaaaga cgcggtgata catatccagc catgcacact
     6601 gatactcttc actccacatg tcggtgtaca ttgagtgcag cccggctaac gtatccacgc
     6661 cgtattcggt gatgataatc ggctgatgca gtttctcctg ccaggccaga agttcttttt
     6721 ccagtacctt ctctgccgtt tccaaatcgc cgctttggac ataccatccg taataacggt
     6781 tcaggcacag cacatcaaag agatcgctga tggtatcggt gtgagcgtcg cagaacatta
     6841 cattgacgca ggtgatcgga cgcgtcgggt cgagtttacg cgttgcttcc gccagtggcg
     6901 cgaaatattc ccgtgcacct tgcggacggg tatccggttc gttggcaata ctccacatca
     6961 ccacgcttgg gtggtttttg tcacgcgcta tcagctcttt aatcgcctgt aagtgcgctt
     7021 gctgagtttc cccgttgact gcctcttcgc tgtacagttc tttcggcttg ttgcccgctt
     7081 cgaaaccaat gcctaaagag aggttaaagc cgacagcagc agtttcatca atcaccacga
     7141 tgccatgttc atctgcccag tcgagcatct cttcagcgta agggtaatgc gaggtacggt
     7201 aggagttggc cccaatccag tccattaatg cgtggtcgtg caccatcagc acgttatcga
     7261 atcctttgcc acgcaagtcc gcatcttcat gacgaccaaa gccagtaaag tagaacggtt
     7321 tgtggttaat caggaactgt tcgcccttca ctgccactga ccggatgccg acgcgaagcg
     7381 ggtagatatc acactctgtc tggcttttgg ctgtgacgca cagttcatag agataacctt
     7441 cacccggttg ccagaggtgc ggattcacca cttgcaaagt cccgctagtg ccttgtccag
     7501 ttgcaaccac ctgttgatcc gcatcacgca gttcaacgct gacatcacca ttggccacca
     7561 cctgccagtc aacagacgcg tggttacagt cttgcgcgac atgcgccacc acggtgatat
     7621 cgtccaccca ggtgttcggc gtggtgtaga gcattacgct gcgatggatt ccggcatagt
     7681 taaagaaatc atggaagtaa gactgctttt tcttgccgtt ttcgtcggta atcaccattc
     7741 ccggcgggat agtctgccag ttcagttcgt tgttcacaca aacggtgata cgtacacttt
     7801 tcccggcaat aacatacggc gtgacatcgg cttcaaatgg cgtatagccg ccctgatgct
     7861 ccatcacttc ctgattattg acccacactt tgccgtaatg agtgaccgca tcgaaacgca
     7921 gcacgatacg ttggcctgcc caacctttcg gtataaagac ttcgcgctga taccagacgt
     7981 tgcccgcata attacgaata tctgcatcgg cgaactgatc gttaaaactg cctggcacag
     8041 caattgcccg gctttcttgt aacgcgcttt cccaccaacg ctgatcaatt ccacagtttt
     8101 cgcgatccag actgaatgcc cacaggccgt cgagtttttt gatttcacgg gttggggttt
     8161 ctacaggacg gaccatggtc gactcctcct tagctggatc ttgatcccct gcgccatcag
     8221 atccttggcg gcaagaaagc catccagttt actttgcagg gctccccaac cttaccagag
     8281 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
     8341 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
     8401 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
     8461 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaattc
     8521 gagcgataag ctcatggagc ggcgtaaccg tcgcacagga aggacagaga aagcgcggat
     8581 ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc
     8641 tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc attcctatgc
     8701 gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga tgggacataa
     8761 gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg ctgcccggca
     8821 ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac aattatcctg
     8881 agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg ctgtttttgt
     8941 ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag tcgggaaaat
     9001 ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc gtttatagga
     9061 agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat atgccttcag
     9121 gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg tcagcaatgg
     9181 acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca gccagtagtg
     9241 ctcgccgcag tcgagcgaca gggcgaagcc ctcggctggt tgccctcgcc gctgggctgg
     9301 cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc
     9361 gcggccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     9421 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     9481 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     9541 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     9601 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     9661 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     9721 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     9781 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     9841 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggtcgagtga
     9901 gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg cctgaaaaaa
     9961 cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt tttttatagt
    10021 ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt tctagagaag
    10081 gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca gtcctgtctg
    10141 tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt ttttcacaaa
    10201 gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac ttgtcacact
    10261 tcacatggat ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg taaaaatagc
    10321 ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat atagtctctc ccgggatcaa
    10381 aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat ctgatggcac cctacaggaa
    10441 catgacggta tctgcgagat ccatgttgct aaatatgctg aaatattcgg attgacctct
    10501 gcggaagcca gtaaggatat acggcaggca ttgaagagtt tcgcggggaa ggaagtggtt
    10561 ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct atgaatcttt tccttggttt
    10621 atcaaacgtg cgcacagtcc atccagaggg ctttacagtg tacatatcaa cccatatctc
    10681 attcccttct ttatcgggtt acagaaccgg tttacgcagt ttcggcttag tgaaacaaaa
    10741 gaaatcacca atccgtatgc catgcgttta tacgaatccc tgtgtcagta tcgtaagccg
    10801 gatggctcag gcatcgtctc tctgaaaatc gactggatca tagagcgtta ccagctgcct
    10861 caaagttacc agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg tgttaatgag
    10921 atcaacagca gaactccaat gcgcctctca tacattgaga aaaagaaagg ccgccagacg
    10981 actcatatcg tattttcctt ccgcgatatc acttccatga cgacaggata gtctgagggt
    11041 tatctgtcac agatttgagg gtggttcgtc acatttgttc tgacctactg agggtaattt
    11101 gtcacagttt tgctgtttcc ttcagcctgc atggattttc tcatactttt tgaactgtaa
    11161 tttttaagga agccaaattt gagggcagtt tgtcacagtt gatttccttc tctttccctt
    11221 cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt gatgagggtt gattatcaca
    11281 gtttattact ctgaattggc tatccgcgtg tgtacctcta cctggagttt ttcccacggt
    11341 ggatatttct tcttgcgctg agcgtaagag ctatctgaca gaacagttct tctttgcttc
    11401 ctcgccagtt cgctcgctat gctcggttac acggctgcgg cgagc
//
