LOCUS       V006184                 6219 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006184
VERSION     V006184
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Designer cells programming quorum-sensing interference with human
            pathogens
  JOURNAL   Nat Commun (2018) In press
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2017) Department of Biosystems Science and
            Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 6219)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6219)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-AUG-2017) Department of Biosystems Science and Engineering
            (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..708
                     /gene="mtnN"
                     /label="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /note="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase from Escherichia coli O45:K1 (strain S88 /
                     ExPEC). Accession#: B7MBE2"
     polyA_signal    755..979
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1025..1453
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1467..1796
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             1845..2867
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    3000..3133
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3170..3186)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3194..3210)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3218..3248)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3263..3284)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3572..4157)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4331..5188)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5189..5293)
                     /label="AmpR promoter"
     enhancer        5559..5938
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5939..6142
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        6187..6205
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagcgccca ccatgaaaat cggcattatc ggagccatgg aagaagaagt cacactgctg
       61 agggacaaga tcgagaacag acagacaatt agcctggggg ggtgcgagat ctacaccggc
      121 cagctgaacg ggacagaagt cgcactgctg aagtccggaa ttggcaaagt ggcagctgca
      181 ctgggagcca cactgctgct ggagcactgt aagccagacg tgatcattaa tacagggtca
      241 gctggcgggc tggcacccac cctgaaagtg ggcgacatcg tggtcagcga tgaggctagg
      301 tatcatgacg ccgatgtgac tgctttcgga tacgaatatg gacagctgcc tggatgccct
      361 gcaggcttca aggccgacga taaactgatc gccgctgcag aggcatgtat tgccgaactg
      421 aacctgaatg ccgtccgggg actgatcgtg tctggggacg ccttcattaa cgggagtgtc
      481 ggactggcta agatcagaca caattttcct caggccattg ctgtggagat ggaagcaacc
      541 gccatcgctc acgtctgcca taacttcaat gtgccctttg tggtcgtgcg ggctattagt
      601 gacgtggcag atcagcagag ccatctgtcc ttcgatgaat ttctggccgt ggccgctaaa
      661 cagtcctccc tgatggtgga atccctggtg cagaagctgg ctcacgggta actcgagtct
      721 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      781 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      841 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      901 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
      961 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     1021 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1081 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     1141 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1201 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     1261 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1321 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     1381 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     1441 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     1501 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     1561 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     1621 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     1681 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     1741 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     1801 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgatgaaa aagcctgaac
     1861 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     1921 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     1981 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     2041 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     2101 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     2161 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     2221 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     2281 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     2341 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     2401 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     2461 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     2521 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     2581 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     2641 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     2701 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     2761 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     2821 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtgctac
     2881 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     2941 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     3001 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3061 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3121 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     3181 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3241 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3301 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     3361 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4081 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     4141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     5341 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5401 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     5461 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     5521 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     5581 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     5641 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     5701 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     5761 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     5821 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     5881 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     5941 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     6001 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     6061 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     6121 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
     6181 cgaaattaat acgactcact atagggagac ccaagctgg
//
