LOCUS       V006185                 6035 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006185
VERSION     V006185
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Designer cells programming quorum-sensing interference with human
            pathogens
  JOURNAL   Nat Commun (2018) In press
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2017) Department of Biosystems Science and
            Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 6035)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6035)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-AUG-2017) Department of Biosystems Science and Engineering
            (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..524
                     /gene="luxS"
                     /label="S-ribosylhomocysteine lyase"
                     /note="S-ribosylhomocysteine lyase from Escherichia coli
                     (strain K12). Accession#: P45578"
     polyA_signal    571..795
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      841..1269
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1283..1612
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             1661..2683
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    2816..2949
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2986..3002)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3010..3026)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3034..3064)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3079..3100)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3388..3973)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4147..5004)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5005..5109)
                     /label="AmpR promoter"
     enhancer        5375..5754
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5755..5958
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        6003..6021
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagcgccac catgccactg ctggattcct ttaccgtcga tcacactcgg atggaagccc
       61 ccgccgtccg cgtcgccaaa acaatgaaca ctcctcacgg cgacgccatc accgtgttcg
      121 atctgaggtt ttgcgtgccc aacaaggagg tcatgcctga acgcgggatt cacaccctgg
      181 agcatctgtt cgctggcttc atgcggaatc acctgaacgg caatggggtc gaaatcattg
      241 acatctctcc aatgggatgt cgaaccggct tctacatgag cctgattggc acacccgacg
      301 agcagagagt ggcagatgcc tggaaggccg ctatggaaga cgtgctgaaa gtccaggatc
      361 agaaccagat ccccgagctg aatgtctacc agtgcgggac atatcagatg catagcctgc
      421 aggaggctca ggacatcgca cggtccattc tggaacggga tgtgagaatc aatagcaatg
      481 aggaactggc tctgcccaag gagaaactgc aggaactgca catctaactc gagtctagag
      541 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      601 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      661 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      721 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
      781 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
      841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      901 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1021 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1141 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1201 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1261 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     1321 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     1381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     1441 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1501 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     1561 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     1621 gggagcttgt atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac
     1681 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     1741 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     1801 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     1861 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     1921 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     1981 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     2041 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     2101 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     2161 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     2221 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     2281 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     2341 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     2401 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     2461 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     2521 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     2581 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     2641 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tgctacgaga
     2701 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     2761 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     2821 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2881 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2941 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     3001 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3061 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3121 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3181 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3241 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3361 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3841 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3901 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3961 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4021 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4081 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4141 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4201 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4261 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4321 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4381 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4441 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4501 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4561 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4621 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4681 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4741 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4801 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4861 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4921 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4981 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5041 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5101 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     5161 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     5221 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     5281 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     5341 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     5401 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     5461 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     5521 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     5581 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     5641 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     5701 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     5761 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     5821 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     5881 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     5941 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
     6001 attaatacga ctcactatag ggagacccaa gctgg
//
