LOCUS       V006186                 4205 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006186
VERSION     V006186
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Designer cells programming quorum-sensing interference with human
            pathogens
  JOURNAL   Nat Commun (2018) In press
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2017) Department of Biosystems Science and
            Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 4205)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-AUG-2017) Department of Biosystems Science and Engineering
            (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..521
                     /gene="luxS"
                     /label="S-ribosylhomocysteine lyase"
                     /note="S-ribosylhomocysteine lyase from Escherichia coli
                     (strain K12). Accession#: P45578"
     polyA_signal    complement(563..684)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      787..1032
                     /label="simian virus 40 polyA"
                     /note="simian virus 40 polyA"
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      complement(1349..1937)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2111..2968)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2969..3073)
                     /label="AmpR promoter"
     rep_origin      3100..3555
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    3686..3734
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    3748..3839
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    4071..4100
                     /label="NFAT binding site"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    4101..4130
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    4131..4160
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     promoter        4173..4204
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
ORIGIN
        1 agcttaccat gccactgctg gattccttta ccgtcgatca cactcggatg gaagcccccg
       61 ccgtccgcgt cgccaaaaca atgaacactc ctcacggcga cgccatcacc gtgttcgatc
      121 tgaggttttg cgtgcccaac aaggaggtca tgcctgaacg cgggattcac accctggagc
      181 atctgttcgc tggcttcatg cggaatcacc tgaacggcaa tggggtcgaa atcattgaca
      241 tctctccaat gggatgtcga accggcttct acatgagcct gattggcaca cccgacgagc
      301 agagagtggc agatgcctgg aaggccgcta tggaagacgt gctgaaagtc caggatcaga
      361 accagatccc cgagctgaat gtctaccagt gcgggacata tcagatgcat agcctgcagg
      421 aggctcagga catcgcacgg tccattctgg aacgggatgt gagaatcaat agcaatgagg
      481 aactggctct gcccaaggag aaactgcagg aactgcacat ctaactcgag tctagagggc
      541 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
      601 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      661 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      721 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
      781 gataaggatc tgaacgatgg agcggagaat gggcggaact gggcggagtt aggggcggga
      841 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
      901 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
      961 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     1021 cattccacag cggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     1081 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     1141 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1201 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1261 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1321 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1981 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2041 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2101 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2161 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2221 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2281 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2341 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2401 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2461 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2521 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2581 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2641 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2701 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2761 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2821 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2881 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2941 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3001 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3061 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     3121 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3181 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3241 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3301 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3361 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3421 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3481 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3541 taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     3601 tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg
     3661 tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     3721 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
     3781 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta
     3841 tcgataggta ccgagctctt acgcgtgcta gcccgggctc gagcgcacca gacagtgacg
     3901 tcagctgcca gatcccatgg ccgtcatact gtgacgtctt tcagacaccc cattgacgtc
     3961 aatgggagaa ccccggtacc gggaggatgt ccatattagg acatctagga tgtccatatt
     4021 aggacatcta ggatgtccat attaggacat ctcccgggga gctcgctagc ggaggaaaaa
     4081 ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa
     4141 ctgtttcata cagaaggcgt agatctagac tctagagggt atataatgga agctcgaatt
     4201 ccaga
//
