LOCUS       V006187                 4714 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006187
VERSION     V006187
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Designer cells programming quorum-sensing interference with human
            pathogens
  JOURNAL   Nat Commun (2018) In press
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2017) Department of Biosystems Science and
            Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 4714)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4714)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-AUG-2017) Department of Biosystems Science and Engineering
            (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             15..1136
                     /gene="GNA15"
                     /label="Guanine nucleotide-binding protein subunit
                     alpha-15"
                     /note="Guanine nucleotide-binding protein subunit alpha-15
                     from Homo sapiens. Accession#: P30679"
     polyA_signal    complement(1186..1307)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1972..2560)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2734..3591)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3592..3696)
                     /label="AmpR promoter"
     rep_origin      3723..4178
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4309..4357
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4371..4462
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        4517..4713
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
ORIGIN
        1 agcttggtac cgagatggcc cgctcgctga cctggcgctg ctgcccctgg tgcctgacgg
       61 aggatgagaa ggccgccgcc cgggtggacc aggagatcaa caggatcctc ttggagcaga
      121 agaagcagga ccgcggggag ctgaagctgc tgcttttggg cccaggcgag agcgggaaga
      181 gcaccttcat caagcagatg cggatcatcc acggcgccgg ctactcggag gaggagcgca
      241 agggcttccg gcccctggtc taccagaaca tcttcgtgtc catgcgggcc atgatcgagg
      301 ccatggagcg gctgcagatt ccattcagca ggcccgagag caagcaccac gctagcctgg
      361 tcatgagcca ggacccctat aaagtgacca cgtttgagaa gcgctacgct gcggccatgc
      421 agtggctgtg gagggatgcc ggcatccggg cctactatga gcgtcggcgg gaattccacc
      481 tgctcgattc agccgtgtac tacctgtccc acctggagcg catcaccgag gagggctacg
      541 tccccacagc tcaggacgtg ctccgcagcc gcatgcccac cactggcatc aacgagtact
      601 gcttctccgt gcagaaaacc aacctgcgga tcgtggacgt cgggggccag aagtcagagc
      661 gtaagaaatg gatccattgt ttcgagaacg tgatcgccct catctacctg gcctcactga
      721 gtgaatacga ccagtgcctg gaggagaaca accaggagaa ccgcatgaag gagagcctcg
      781 cattgtttgg gactatcctg gaactaccct ggttcaaaag cacatccgtc atcctctttc
      841 tcaacaaaac cgacatcctg gaggagaaaa tccccacctc ccacctggct acctatttcc
      901 ccagtttcca gggccctaag caggatgctg aggcagccaa gaggttcatc ctggacatgt
      961 acacgaggat gtacaccggg tgcgtggacg gccccgaggg cagcaagaag ggcgcacgat
     1021 cccgacgcct cttcagccac tacacatgtg ccacagacac acagaacatc cgcaaggtct
     1081 tcaaggacgt gcgggactcg gtgctcgccc gctacctgga cgagatcaac ctgctgtgac
     1141 ctcgagtcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     1201 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     1261 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     1321 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     1381 acctctacaa atgtggtaaa atcgataagg atctgaacga tggagcggag aatgggcgga
     1441 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     1501 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     1561 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     1621 ctttccacac cctaactgac acacattcca cagcggatcc gtcgaccgat gcccttgaga
     1681 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     1741 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
     1801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2401 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2521 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2641 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2701 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2761 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2821 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2881 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2941 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3001 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3061 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3601 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3661 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3721 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3901 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3961 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4021 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4081 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4141 taacgcgaat tttaacaaaa tattaacgtt tacaatttcc cattcgccat tcaggctgcg
     4201 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc
     4261 atgataagta agtaatatta aggtacggga ggtacttgga gcggccgcaa taaaatatct
     4321 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
     4381 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
     4441 gcaggtgcca gaacatttct ctatcgatag gtaccgagct cttacgcgtg ctagcccggg
     4501 ctcgagatct gcgatctgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4561 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatcg ctgactaatt
     4621 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4681 ggaggctttt ttggaggcct aggcttttgc aaaa
//
