LOCUS       40924_20541        3084 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFS-sigHis-PGK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Wilke S, Gossen M, Wirth D, Buessow K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2011) Structural Biology, Helmholtz Centre for 
            Infection Research, Inhoffenstr. 7, Braunschweig 38124, Germany
REFERENCE   2  (bases 1 to 3084)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2011) Structural Biology, Helmholtz Centre for Infection 
            Research, Inhoffenstr. 7, Braunschweig 38124, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     25..72
                     /note="F3 site; synthetic variant 3 of the yeast
                     flp-recombinase target (FRT) site"
     CDS             91..147
                     /label=IgH signal sequence
                     /note="signal sequence from the mouse immunoglobulin heavy
                     chain variable region"
     misc_feature    148..175
                     /label=BpiI cloning site
                     /note="BpiI cloning site"
     CDS             176..193
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    complement(211..345)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        683..1193
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     protein_bind    1220..1267
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      complement(1332..1920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2094..2951)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2952..3056)
                     /label=AmpR promoter
     primer_bind     3067..3084
                     /label=pBR1
                     /note="pBR1"
ORIGIN
        1 gtcgacggat cgggagatca gcttgaagtt cctattccga agttcctatt cttcaaatag
       61 tataggaact tcgaatttcc ggtcgccacc atgggctgga gctgcatcat tctgttcctc
      121 gtggctacag ccaccggtgt tcactcctag tcttcagatc tgctagcgaa gacttcatca
      181 tcatcaccac cactgaacgc gtatttaaat gatccagaca tgataagata cattgatgag
      241 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      301 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      361 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      421 ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg
      481 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      541 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
      601 ccatgaccct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
      661 gcggggcatg actaaattcc acggggttgg ggttgcgcct tttccaaggc agccctgggt
      721 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
      781 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
      841 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
      901 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
      961 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     1021 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     1081 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     1141 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     1201 caccggtcgc caacatggag aagttcctat tccgaagttc ctattctcta gaaagtatag
     1261 gaacttctcg acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1321 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1381 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1441 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1501 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     1561 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1621 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1681 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1741 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     1801 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1861 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1921 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1981 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2041 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2101 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2161 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2221 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2281 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2341 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2401 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2461 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2521 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2581 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2641 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2701 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2761 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2821 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2881 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2941 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3001 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3061 tttccccgaa aagtgccacc tgac
//
