LOCUS       40924_20531        6042 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Insertion vector pFRT2-lacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Son MS, Nguyen DT, Kang Y, Hoang TT.
  TITLE     Engineering of FRT-lacZ fusion constructs: induction of the 
            Pseudomonas aeruginosa fadAB1 operon by medium and long chain-length
            fatty acids
  JOURNAL   Plasmid 59 (2), 111-118 (2008)
  PUBMED    18221997
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Son MS, Kang Y, Nguyen DT, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2007) Microbiology, University of Hawaii Manoa, 
            2538 McCarthy Mall, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 6042)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6042)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2008"; volume: "59"; issue: "2"; pages: "111-118"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUL-2007) Microbiology, University of Hawaii Manoa, 2538 
            McCarthy Mall, Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(190..299)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      complement(720..747)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(839..925)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1398..4442)
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSADFQLSAGRYHYQLVWCQK"
     protein_bind    complement(4519..4566)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        4680..4708
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4897..5427
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      5633..6018
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 acgttaaggg attttggtca tggacggcca gcaggtaggc cgacaggctc atgccggccg
       61 ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc ttgataggtg
      121 ggctgccctt cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc
      181 cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat
      241 aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg
      301 gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat
      361 atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt
      421 atgcagcgga aaagcgctgc ttccctgctg ttttgtggaa tatctaccga ctggaaacag
      481 gcaaatgcag gaaattactg aactgagggg acaggcgaga gacgatgcca aagagctaca
      541 ccgacgagct ggccgagtgg gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg
      601 ctgcggttgc gttcctggcg gtgagggcgg atgtcgatat gcgtaaggag aaaataccgc
      661 atcaggcgca tatttgaatg tatttagaaa aataaacaaa aagagtttgt agaaacgcaa
      721 aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt tatggcgggc
      781 gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat
      841 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      901 tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac
      961 cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg
     1021 accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat
     1081 ttaatctgta tcaggctgaa aattaaggaa tcccccagga cccaacgctg cccgagtttg
     1141 tcagaaagca gaccaaacag cggttggaat aatagcgaga acagagaaat agcggcaaaa
     1201 ataatacccg tatcactttt gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac
     1261 gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag
     1321 tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg cagacatggc
     1381 ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg accggcgctc
     1441 agctggaaat ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccatatgg
     1501 aaaccgtcga tattcagcca tgtgccttct tccgcgtgca gcagatggcg atggctggtt
     1561 tccatcagtt gctgttgact gtagcggctg atgttgaact ggaagtcgcc gcgccactgg
     1621 tgtgggccat aattcaattc gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg
     1681 tacggggtat acatgtctga caatggcaga tcccagcggt caaaacaggc ggcagtaagg
     1741 cggtcgggat agttttcttg cggccctaat ccgagccagt ttacccgctc tgctacctgc
     1801 gccagctggc agttcaggcc aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca
     1861 tcaacggtaa tcgccatttg accactacca tcaatccggt aggttttccg gctgataaat
     1921 aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt
     1981 gtatctgccg tgcactgcaa caacgctgct tcggcctggt aatggcccgc cgccttccag
     2041 cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc
     2101 agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc
     2161 cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt acccagctcg
     2221 atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc
     2281 gtcacactga ggttttccgc cagacgccac tgctgccagg cgctgatgtg cccggcttct
     2341 gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga gccagagttg cccggcgctc
     2401 tccggctgcg gtagttcagg cagttcaatc aactgtttac cttgtggagc gacatccaga
     2461 ggcacttcac cgcttgccag cggcttacca tccagcgcca ccatccagtg caggagctcg
     2521 ttatcgctat gacggaacag gtattcgctg gtcacttcga tggtttgccc ggataaacgg
     2581 aactggaaaa actgctgctg gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg
     2641 gcaaagacca gaccgttcat acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg
     2701 ccgtaagccg accacgggtt gccgttttca tcatatttaa tcagcgactg atccacccag
     2761 tcccagacga agccgccctg taaacgggga tactgacgaa acgcctgcca gtatttagcg
     2821 aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc
     2881 tctccaggta gcgaaagcca ttttttgatg gaccatttcg gcacagccgg gaagggctgg
     2941 tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg tggccgtggt gtcggctccg
     3001 ccgccttcat actgcaccgg gcgggaagga tcgacagatt tgatccagcg atacagcgcg
     3061 tcgtgattag cgccgtggcc tgattcattc cccagcgacc agatgatcac actcgggtga
     3121 ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga
     3181 tcatcggtca gacgattcat tggcaccatg ccgtgggttt caatattggc ttcatccacc
     3241 acatacaggc cgtagcggtc gcacagcgtg taccacagcg gatggttcgg ataatgcgaa
     3301 cagcgcacgg cgttaaagtt gttctgcttc atcagcagga tatcctgcac catcgtctgc
     3361 tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc
     3421 aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca
     3481 tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac caccgcacga
     3541 tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag acgtagtgtg
     3601 acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg cgcggtgccg
     3661 ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta gtcacgcaac
     3721 tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc attaaagcga
     3781 gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga gacgtcacgg
     3841 aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc actccaacgc
     3901 agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca
     3961 gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca cagatgaaac
     4021 gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca
     4081 acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa cggcggattg
     4141 accgtaatgg gataggttac gttggtgtag atgggcgcat cgtaaccgtg catctgccag
     4201 tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca gctttccggc
     4261 accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt
     4321 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     4381 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     4441 acgggatcag ccattttttt ctccttactt acttaggatc cccgggtacc gagctcgaat
     4501 tagcttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
     4561 aacttcaaga tccccaattc gagctcggta cccggggatc ccccgggctc gactgcatta
     4621 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgctcgaat
     4681 tgacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact
     4741 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     4801 gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     4861 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag caacgatgtt
     4921 acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg catcattcgc
     4981 acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt
     5041 cgtgagttcg gagacgtagc cacctactcc caacatcagc cggactccga ttacctcggg
     5101 aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga agcggttgtt
     5161 ggcgctctcg cggcttacgt tctgcccagg tttgagcagc cgcgtagtga gatctatatc
     5221 tatgatctcg cagtctccgg cgagcaccgg aggcagggca ttgccaccgc gctcatcaat
     5281 ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca agcagattac
     5341 ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga agtgatgcac
     5401 tttgatatcg acccaagtac cgccacctaa caattcgttc aagccgagat cggcttcccg
     5461 gccgcggagt tgttcggtaa attgtcacaa cgccgcggcc aattcggctt cctcgctcac
     5521 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5581 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag atcgatcagc
     5641 agttcaacct gttgatagta cgtactaagc tctcatgttt cacgtactaa gctctcatgt
     5701 ttaacgtact aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc
     5761 tcatggctaa cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata
     5821 aaattaatat aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa
     5881 aagaatatat aaggctttta aagctagctt ttaaggttta acggttgtgg acaacaagcc
     5941 agggatgtaa cgcactgaga agcccttaga gcctctcaaa gcaattttca gtgacacagg
     6001 aacacttaac ggctgacatg acgctcagtg gaacgaaaac tc
//
