LOCUS       40924_20521       11266 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fusion vector pFRT1-lux-Tel, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Wilcox BA, Hoang TT.
  TITLE     Engineering of tellurite-resistant genetic tools for single-copy 
            chromosomal analysis of Burkholderia spp. and characterization of 
            the Burkholderia thailandensis betBA operon
  JOURNAL   Appl. Environ. Microbiol. 75 (12), 4015-4027 (2009)
  PUBMED    19376905
REFERENCE   2  (bases 1 to 11266)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Videau PJA., Wilcox BA, Hoang TT.
  TITLE     Genetic Systems for Single Copy Analysis based on a Non-antibiotic 
            Selectable Marker in Burkholderia spp.
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11266)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Videau PJA., Wilcox BA, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2008) Microbiology, University of Hawaii at Manoa,
            2538 McCarthy Mall, Snyder Hall 207, Honolulu, HI 96822, USA
REFERENCE   4  (bases 1 to 11266)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11266)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2009"; volume: "75"; issue: "12"; 
            pages: "4015-4027"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2008) Microbiology, University of Hawaii at Manoa, 2538 
            McCarthy Mall, Snyder Hall 207, Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(190..299)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      complement(720..747)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(839..925)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1549..2658)
                     /label=LuxE
                     /note="LuxE"
     CDS             complement(2840..3820)
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             complement(3838..4917)
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             complement(4969..5889)
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             complement(5904..7343)
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     protein_bind    7400..7447
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7558..7586
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     terminator      7710..7741
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(7759..8712)
                     /codon_start=1
                     /gene="telB"
                     /product="TelB"
                     /label=telB
                     /note="tellurite resistance"
                     /protein_id="ACJ70724.1"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     gene            complement(7759..8712)
                     /gene="telB"
                     /label=telB
     CDS             complement(8709..9845)
                     /codon_start=1
                     /gene="telA"
                     /product="TelA"
                     /label=telA
                     /note="tellurite resistance"
                     /protein_id="ACJ70725.1"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     gene            complement(8709..9845)
                     /gene="telA"
                     /label=telA
     CDS             complement(9863..10636)
                     /codon_start=1
                     /gene="kilA"
                     /product="KilA"
                     /label=kilA
                     /note="tellurite resistance"
                     /protein_id="ACJ70726.1"
                     /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL
                     MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL
                     LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF
                     DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA
                     LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     gene            complement(9863..10636)
                     /gene="kilA"
                     /label=kilA
     RBS             complement(10643..10665)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      complement(10676..10717)
                     /note="derived from Burkholderia cenocepacia rpsL promoter"
                     /regulatory_class="promoter"
     rep_origin      10857..11242
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 acgttaaggg attttggtca tggacggcca gcaggtaggc cgacaggctc atgccggccg
       61 ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc ttgataggtg
      121 ggctgccctt cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc
      181 cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat
      241 aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg
      301 gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat
      361 atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt
      421 atgcagcgga aaagcgctgc ttccctgctg ttttgtggaa tatctaccga ctggaaacag
      481 gcaaatgcag gaaattactg aactgagggg acaggcgaga gacgatgcca aagagctaca
      541 ccgacgagct ggccgagtgg gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg
      601 ctgcggttgc gttcctggcg gtgagggcgg atgtcgatat gcgtaaggag aaaataccgc
      661 atcaggcgca tatttgaatg tatttagaaa aataaacaaa aagagtttgt agaaacgcaa
      721 aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt tatggcgggc
      781 gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat
      841 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      901 tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac
      961 cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg
     1021 accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat
     1081 ttaatctgta tcaggctgaa aattaaggaa tcccccagga cccaacgctg cccgagtttg
     1141 tcagaaagca gaccaaacag cggttggaat aatagcgaga acagagaaat agcggcaaaa
     1201 ataatacccg tatcactttt gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac
     1261 gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag
     1321 tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg cagacatggc
     1381 ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg accggcgctc
     1441 agctggaaat ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccataaat
     1501 tcgagctcgg tacccgggga tcctctagag tcgacctgca ggtcatcaac tatcaaacgc
     1561 ttcggttaag cttaaagcac accctttctg cgtcctcgta ttgacgcgac gtaaaatttc
     1621 aacgagcacg ccgggatact taccatattc tctgctaatt atcccgacat catcggtaac
     1681 aataaatgct ggataactgg ttgctgacgc atccatataa ctcatcaacc ccggcgttcc
     1741 atcaggtaca ggtttcaacg tttcaggatc aagcgctcgc gcatataccc acggcggaac
     1801 atgtttacgc tgcatttcat cctcaaagaa acaagtgttg agttcaactt gattaaatat
     1861 atctcggatc tgactaatat cactgagatt gaaagtatca aataaaagat gattgaaatc
     1921 atcacgtttc agagattctt tttcgtaact tttccagccg cctccggtta tgatataaag
     1981 gcttttatct ccagaaaatg agattttttt atctttcata taatggcaga gtaaataaat
     2041 aaagtatggc gaaccaataa gacaaagatc tttcccttga ttttttattc gttcaagact
     2101 attcaatgtt ttaacaaaat ctattcgttc ttctgttacg gtaaatgtcg taggatataa
     2161 caattccacc aaactcataa catatttaaa ccaaatatta tgagcattaa atctatctgg
     2221 tcccaaattg actaattcta tttgatgatc aaaccaacta ccaacatatt tcatgccata
     2281 actcacagag cctaagagtc tctcaatact taatctgtca cgcgccacct gactttttaa
     2341 accattcgtg ccgctactgg taaaccaact ttcaatctcg ttttcctgag aagttaataa
     2401 gcgagtaaac ttaaaaaccg atgttgggaa tacaggtatg tcatcaattt ccgtaatatt
     2461 gtcatctact ttgtgtgcct gacagtagtg acgatattct cgacaatgtt tataatgatt
     2521 acgaaatgca tcaagcacaa gtttctttct gattttttcc tgctcgtcgt aagaccacac
     2581 taatggatcg ctcgaaaaaa tcaaatcatc aatttctgag cttgctgtaa tttcttgttt
     2641 atcaacatat gaagtcatac ctgttttact cctcaagata atattagaaa gtatggcagc
     2701 actgctgtca tactctttta tacccttcat ctttcaagct gctgctttgt tggctgcttt
     2761 cactcacccc agtcacatag ttatctatgc tcctggggat tcgttcactt gccgcctcgc
     2821 tgcaactcga aatctattag gtatattcca tgtggtactt cttaatatta tcatcaacaa
     2881 tattgattac atttttttgg ctcatcaaat cattcattgg ttcaaaggac agcaatacac
     2941 ttttcgcacc acacttttca attgccaact tagccgcagt tatacactcc gtataatttc
     3001 cgacagcgtt ttctgcaatt atttcttcaa gtttattttc gaaattttca ttagggtgca
     3061 tttcaagaac ataatcacta ataaatgcac gcgtctcttg tttagcttta ttactatctt
     3121 cgttatagtt aactaatatc attaactgat ggtctatctc tgataggtca acgtcatatt
     3181 tatccgcaac ggctttatat ctttcagcat attcatatct aacatcatta gaatcatccc
     3241 acttaaagat gagaggaata ccttttttgg ccgcccactc aacaatatga tgactggttg
     3301 ctgttacata tttccgaggt ccgcctggcg tataagcatg gggatttaca gatattttag
     3361 ggaagctata aaaatcgtta tctggattac aatagcctgt tgttaaagca tcgttaatga
     3421 tttcataaca ctcttcaaat agttgctgtt gatattcaac cgggcgatta aaaaaatgca
     3481 tttcatcttt tttttcgcaa tcactaaacc ctaaaataaa tctcccttca cttaactgat
     3541 ccaataagca agcttcctcc gctatggcga caggatgatg agttgtaatg atgtgattta
     3601 atgaaccaat tttaattttc tctgttaaac cgagcagaaa accagaaaca gtcagaggag
     3661 cgccgacaac accattatct gaaaaatgat tttcatacac taaaatctgt tcaaaattca
     3721 acttatcaac atactccgtt atttcctgca tgcgaactat actttgttct tgaacagttg
     3781 ttgaattgat gaagttaagg aagaacaatc caaatttcat ttctttctcc ttagctaata
     3841 taatagcgaa cgttgttttt ctttaagaaa tggcatgaca tcagactgga agagcttcat
     3901 ggaagcaata atttcgtcta ctgttccatt agcttcaaat ccacaacaaa tatttgatat
     3961 tcctgtagca tcaatgtctt tttgaattat gtcaatacat tcctgcggcg ttcccacggg
     4021 attgatttcg taactgtaat caatacggcg attagtatct ttatgtcctt ttaatacaaa
     4081 gtcacgccac tgccctttat tgaaatcata acctcttgtt tggtctgaat catcaaaaat
     4141 agtcgtagca ttcacataag aatcatacca atgccccaga aatttccggc aaatctcttt
     4201 cgctttaatt gagtcatgat ctacagatgt tatatatgat aagcaatggt cgatattatg
     4261 aatatcgtgc ccatattctt gagccacttc attataaagc tcaagttgtg ctttcttttc
     4321 gttagtattt ataatccaac ttaatatcat cggtaggcca aattgagcag cccactcagt
     4381 cgtcgaagct gattcagcca ccacataaac cggtgcgcca cctctgctat acgccgcggg
     4441 gtttactttt accttatgga acttgatatg ttcattatca gcttccatat atccctctgt
     4501 catgccattc tttatcagcc cgtaccagca ttccgctaag gcgcgactgt tattcatatc
     4561 tgtgccgaat acgcgaaagt ccttgttgta aagccctcgg caaataccaa accgaaatcg
     4621 tccttttgac atttgatcca ataaattcac atcttcaagt tggcgtactg gatgggctgt
     4681 gggaagaaca atagcggcag ttcctacatt caatttttta gtcgcgccaa gtaaatatgc
     4741 agcagcgaca taagggttac caagcaaacc aaactccgtg aaatgatgct ccagtaacca
     4801 tacggtatca aaaccacact cctcagagat gcgacctaat ttaaccaaac gtttcattac
     4861 ctctgtttga gaaaattggg gaggttggta tgtaagcaaa aagtttccaa atttcataga
     4921 gagtccttat attgctattt gagtgataga atatctcaat agattttaag acagagaaat
     4981 tgcttgattt tcaatctcaa ttctcattcg gcgttcattg actgtcgcaa tagttaaatg
     5041 ttcaaatgac ggttcagtaa tatcaacatc aatatccaga tgatcattat ccatcgcgat
     5101 agcggctttc gtaaccgatt gataaaaatt gcgcaggacc actaaatttt cactcaagtc
     5161 atgcgaactt cctaacaaag aatatatctt gcatcgatta ctacgaatat ttgataacaa
     5221 tgtgataact tcatcttgct tgacccaatt atcgttattt gcagtaaaag caataaacgg
     5281 tatatcaaga tacatcatgt tattaattgt agaagctaaa tcttcccaac caaaatcaag
     5341 acaatctctc gcaaagactt cagcacccaa tttatggcct tcaaaatcta gattatccgg
     5401 caattcatta atgggtagac tgagataatc aaaccctaaa gctctttcaa gagaatatct
     5461 taagttaaca acaccgactg cggtgattaa aaacgaagca ttgatttcag ataggcttgc
     5521 ataagctatc cgcgcagata agcttgaagc caacataccg aagttattta tttttcgtgt
     5581 agttaaccaa tcaaccactg ctaacaagct ctgctttcct atagacattg taaattcatc
     5641 aattgtccct gaactcaatc caacgtggtg aagcgaatca tagcggatca catgaaatcc
     5701 attccgcgat aaatattccg ccagaccagc aaaatgatcc atcctgcggg caaaaccaga
     5761 cgcaataata atggcattct ttctctttgg gctgttttct tctggcagcg tttcccaaac
     5821 atgaattttt ttatttcctt caacacaaat aacgtggtcg atggttttat attttgattc
     5881 attttccata cttttaccta ttatgggaca aatacaagga acttatcttc ttccaggaat
     5941 cgagtctgtt ctatttcaac cgcaacatcc ttagccgtat agttagatgg cctttcatga
     6001 gaaatatatg tcactaatcg ttgcaacggt ctcattccgt catgagatcc accaactcga
     6061 aatatgttat tcattcctgc ttctacaatc ctttccgcac cttttaatgc taacgcatct
     6121 cgatatttaa atgatgactc ccaaggaaaa atagatatgg tttgcgtctt atttttttga
     6181 acataaggca atatttgctc aatattatcg acgtgatgaa ggtacacaca tctgccaagt
     6241 ggttgattaa attccacacc tgcatttgac tcaataatca tccaacgttg atgaatatcc
     6301 acctctactt ttaatccagc aaacaagctt tctttttgaa ctaaagaata ggccgccttt
     6361 tcatcaaaat cttttttggc attcggtaat atatgcgcat atagattaag tttttctatc
     6421 aacgctaact taaattcctc ataatgattt cccatgtaat atatgttttg ggcagaaaaa
     6481 caagctcgct gatcgtaaaa acaaacatca tgagccgcac ctgtcgctgc ggacgtcaaa
     6541 tcaacaggat tatcgataat gcaaagactc tttttagaac caaatttaat cacatcagca
     6601 taagatggcg catgctctac cgcccaatta atcgcatctg gccctcccca agcgacaata
     6661 acatccgcat gtcgcataat ttcttttgcg agtgatgtat caccttggtg gggccaatat
     6721 ataacagata aagagcgcgt tatcggatga ttagggtcta catcaataaa acttaacgct
     6781 aatgcattag cggtaaaagg atcggttgac gatgttttta taatacactg attcttagtt
     6841 aaaattgcgc gtaatataga catgatccca gataatggaa cattacctgc caacagatgt
     6901 acagatttac ctttcggaaa agcccgaaca taactttcat cctgaggtag ccattcatcc
     6961 atgatatggc gagaaccaag ttcattttct acaacatcat aaaggccgcc tttagaacat
     7021 aaaatcatag atatccaatt ggcctctagc ttagccattt cttctgaata tcccatatat
     7081 ttttttaagt cacgaatgta tgtcctgcgt cttgagtatt cttcattttt ccatctttgc
     7141 cctaccgtat agagaaaatt gacaatgtta tgcaaccgta attcgttatt tccattacaa
     7201 tcaataatgt tttttacatg agagtcattc aatattggca ggtaaacact attatcacca
     7261 aaattaatgg attgcactaa atcatcactt tcgggaaaga tttcaacctg gccgttaata
     7321 atgaatgaaa tttttttagt catacgtatc ctccaagcct gaattgatcc ccgggtaccg
     7381 agctcgaatt ggggatcttg aagttcctat tccgaagttc ctattctcta gaaagtatag
     7441 gaacttcaga gcgcttttga agctaattcg agctcggtac ccgggctcga ctgcattaat
     7501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gctcgaattg
     7561 acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg
     7621 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     7681 tatgactgtt tttttgtact cgagcagaaa gtcaaaagcc tccgaccgga ggcttttgac
     7741 ttgaggggga tcgatccctt atggctctgc acccggctcc atcaccaaca ggtcgcgcac
     7801 gcgcttcact cggttgcgga tcgacactgc cagcccaaca aagccggttg ccgccgccgc
     7861 caggatcgcg ccgatgatgc cggccacacc ggccatcgcc caccaggtcg ccgccttccg
     7921 gttccattcc tgctggtact gcttcgcaat gctggacctc ggctcaccat aggctgaccg
     7981 ctcgatggcg tatgccgctt ctccccttgg cgtaaaaccc agcgccgcag gcggcattgc
     8041 catgctgccc gccgctttcc cgaccacgac gcgcgcacca ggcttgcggt ccagaccttc
     8101 ggccacggcg agctgcgcaa ggacataatc agccgccgac ttggctccac gcgcctcgat
     8161 cagctcttgc actcgcgcga aatccttggc ctccacggcc gccatgaatc gcgcacgcgg
     8221 cgaaggctcc gcagggccgg cgtcgtgatc gccgccgaga atgcccttca ccaagttcga
     8281 cgacacgaaa atcatgctga cggctatcac catcatgcag acggatcgca cgaacccgca
     8341 gaactcaccc ccgaacacga gcacggcacc cgcgaccact atgccaagaa tgcccaaggt
     8401 aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg acggccgaag tgaagggcag
     8461 gccgccaccc aggccgccgc cctcactgcc cggcacctgg tcgctgaatg tcgatgccag
     8521 cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac
     8581 tgccccgatc ccggcaatgg caaggactgc cagcgccgcg atgaggaagc gggtgccccg
     8641 cttcttcatc ttcgcgcctc gggcctccag gccgcctacc tgggcgaaaa catcggtgtt
     8701 tgtggcattc atacggactc ctgttgggcc agctcgcgca cgggctggcg ggtcagcttg
     8761 gcttgaagat cgccacgcat tgcggcgatc tgcttctcgg catccttgcg cttctgcacg
     8821 ccttcctgct ggatgcgaat aacgtcctcg acggtcttga tgagcgtcgt ctgaacctgc
     8881 ttgagcgtgt ccacgtcgat caccaggcgt tggttctcct tcgccgtctc gacggacgtg
     8941 cgatgcagca gggccgcatt gcgcttcatc aggtcgttgg tggtgtcgtc gatggccgtg
     9001 gccagttcga cggcgttctt ctgctcgttg aggctcaagg ccagcatgaa ttgccgcttc
     9061 cacgccggca cggtgatttc gcggatggtg tggaatttat cgaccagcat ctggttgttg
     9121 gcctggatca tgcggatggt cggcaggctc tgcatggccg aatgttgcaa ggcgatcagg
     9181 tcgccgatgc gcttgtccag gttggcaacc atcgcatcga ggtcggccag ctcctgcacg
     9241 cggccagggt cgttcccgac attgccgcgc agaccctcgg cctgctcgcg cagctcggca
     9301 aggcggacct tgccggccgc gatgtggacg ccaagaaggc ggtgttcctc gcgcacggct
     9361 gcgaacattt cgtcgagcga ggcattgcgc tgcgcgatgc cttgctgggt ggtctgcact
     9421 tcgctgacca ggtgttcgat ctgctcgcgg gtcgtgtcga agcgcgccat gaagcccgtc
     9481 gaacggacgc ggaagcggtc gatcagcggg ccaatcaggg gcaggcggga acggttgtcg
     9541 gacaaagggc cgacgttcag ggaacgggcc ttggcgacaa cctgggtcag tttctcgcct
     9601 gcttcgtcca ggtcgctgtt gcgcacctgg tccagcaggc tatcggcgta gcgggacgtg
     9661 tgctcggcca cgtcgcggcc gaactcggca acggtctgcg gactgccgac ctcgatccgc
     9721 tgcgcgaccg catggacttc cggcacgtcg ctttcctgca agcccagctc gcgcagggtt
     9781 gccggggtca tgtcgaaggc gacgataggg gccttggcgt cgtgcgtcgt tttcagtgcg
     9841 ttcatagggt tctcccgccg tgttattggt tgatgccttc caggctctgc gaaaggctcc
     9901 gcatgagcgc ctggtgagct ttggccgcct cggcgaccat tgccggattc atgttcttgg
     9961 tggtgatgag cgcgagggtg tgctgacgcc agacgggcac caggacggat gccgtttcag
    10021 agaagcggtc cagcatgtcc acggcctgcg cccgcgtgag cttcatctga gtgacgctca
    10081 tttcatggga cgccatgagg gttgccaggt tggcgagctt gcgcgcgaag cgttcgcgcg
    10141 gcttgtcgaa ctcgatcacg ccggccttgg ccgcgccggc ctcggggttc tcgtccagga
    10201 actcgcgccc ggcttgaatg taggctctga gccggtctac ctcggcctca tgcgtattga
    10261 gcatgtcatc caaggcgcgc aacgtgtccc gcacgcgctg cgctacgccc tcggcttcgt
    10321 ccagcaactg gtcgagcgtc ttgcgggcga cctgatacct cacctggcgt tcaacctcac
    10381 ggccaagcat cttctcgaac caggtaggct tttccgcgat cttgcggggg tccgcgtcgg
    10441 ccagcttcgc cacgatctgg ctgattttgt cggccagcgc ggcaactgcg ccgtgctcca
    10501 tcagattcga cagctcgttg agggaatccg ccccgtcgat gccggccccg tactcgccaa
    10561 tcgtcgccgg cgacgcgaag agggcgggca aaacctcccc cttcaatcgc gccatgttca
    10621 cgctttgttc ttccatggta tatctccttc ttaaagttaa acaaaattat tcggaaccca
    10681 gcatgatatt ccggaaatac caactaagtc aacggctgat ggccaattcg gcttcctcgc
    10741 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    10801 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagatcgat
    10861 cagcagttca acctgttgat agtacgtact aagctctcat gtttcacgta ctaagctctc
    10921 atgtttaacg tactaagctc tcatgtttaa cgaactaaac cctcatggct aacgtactaa
    10981 gctctcatgg ctaacgtact aagctctcat gtttcacgta ctaagctctc atgtttgaac
    11041 aataaaatta atataaatca gcaacttaaa tagcctctaa ggttttaagt tttataagaa
    11101 aaaaaagaat atataaggct tttaaagcta gcttttaagg tttaacggtt gtggacaaca
    11161 agccagggat gtaacgcact gagaagccct tagagcctct caaagcaatt ttcagtgaca
    11221 caggaacact taacggctga catgacgctc agtggaacga aaactc
//
