LOCUS       40924_20511        6169 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fusion vector pFRT1-gfp-Tel, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Wilcox BA, Hoang TT.
  TITLE     Engineering of tellurite-resistant genetic tools for single-copy 
            chromosomal analysis of Burkholderia spp. and characterization of 
            the Burkholderia thailandensis betBA operon
  JOURNAL   Appl. Environ. Microbiol. 75 (12), 4015-4027 (2009)
  PUBMED    19376905
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Videau PJA., Wilcox BA, Hoang TT.
  TITLE     Genetic Systems for Single Copy Analysis based on a Non-antibiotic 
            Selectable Marker in Burkholderia spp.
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6169)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Videau PJA., Wilcox BA, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2008) Microbiology, University of Hawaii at Manoa,
            2538 McCarthy Mall, Snyder Hall 207, Honolulu, HI 96822, USA
REFERENCE   4  (bases 1 to 6169)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6169)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2009"; volume: "75"; issue: "12"; 
            pages: "4015-4027"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2008) Microbiology, University of Hawaii at Manoa, 2538 
            McCarthy Mall, Snyder Hall 207, Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(190..299)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      complement(720..747)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(839..925)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1528..2241)
                     /label=GFP
                     /note="green fluorescent protein"
     protein_bind    2303..2350
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        2461..2489
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     terminator      2613..2644
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(2662..3615)
                     /codon_start=1
                     /gene="telB"
                     /product="TelB"
                     /label=telB
                     /note="tellurite resistance"
                     /protein_id="ACJ70712.1"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     gene            complement(2662..3615)
                     /gene="telB"
                     /label=telB
     CDS             complement(3612..4748)
                     /codon_start=1
                     /gene="telA"
                     /product="TelA"
                     /label=telA
                     /note="tellurite resistance"
                     /protein_id="ACJ70713.1"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     gene            complement(3612..4748)
                     /gene="telA"
                     /label=telA
     CDS             complement(4766..5539)
                     /codon_start=1
                     /gene="kilA"
                     /product="KilA"
                     /label=kilA
                     /note="tellurite resistance"
                     /protein_id="ACJ70714.1"
                     /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL
                     MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL
                     LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF
                     DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA
                     LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     gene            complement(4766..5539)
                     /gene="kilA"
                     /label=kilA
     RBS             complement(5546..5568)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      complement(5579..5620)
                     /note="derived from Burkholderia cenocepacia rpsL promoter"
                     /regulatory_class="promoter"
     rep_origin      5760..6145
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 acgttaaggg attttggtca tggacggcca gcaggtaggc cgacaggctc atgccggccg
       61 ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc ttgataggtg
      121 ggctgccctt cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc
      181 cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat
      241 aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg
      301 gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat
      361 atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt
      421 atgcagcgga aaagcgctgc ttccctgctg ttttgtggaa tatctaccga ctggaaacag
      481 gcaaatgcag gaaattactg aactgagggg acaggcgaga gacgatgcca aagagctaca
      541 ccgacgagct ggccgagtgg gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg
      601 ctgcggttgc gttcctggcg gtgagggcgg atgtcgatat gcgtaaggag aaaataccgc
      661 atcaggcgca tatttgaatg tatttagaaa aataaacaaa aagagtttgt agaaacgcaa
      721 aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt tatggcgggc
      781 gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat
      841 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      901 tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac
      961 cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg
     1021 accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat
     1081 ttaatctgta tcaggctgaa aattaaggaa tcccccagga cccaacgctg cccgagtttg
     1141 tcagaaagca gaccaaacag cggttggaat aatagcgaga acagagaaat agcggcaaaa
     1201 ataatacccg tatcactttt gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac
     1261 gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag
     1321 tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg cagacatggc
     1381 ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg accggcgctc
     1441 agctggaaat ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccataagc
     1501 ttgcatgcct gcaggtctgg acatttattt gtatagttca tccatgccat gtgtaatccc
     1561 agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga
     1621 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
     1681 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct
     1741 aattttgaag ttaactttga ttccattctt ttgtttgtct gccatgatgt atacattgtg
     1801 tgagttatag ttgtattcca atttgtgtcc aagaatgttt ccatcttctt taaaatcaat
     1861 accttttaac tcgattctat taacaagggt atcaccttca aacttgactt cagcacgtgt
     1921 cttgtagttc ccgtcatctt tgaaaaatat agttctttcc tgtacataac cttcgggcat
     1981 ggcactcttg aaaaagtcat gctgtttcat atgatctggg tatcttgaaa agcattgaac
     2041 accataagtc agagtagtga caagtgttgg ccatggaaca ggtagttttc cagtagtgca
     2101 aataaattta agggtaagtt ttccgtatgt tgcatcacct tcaccctctc cactgacaga
     2161 aaatttgtgc ccattaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa
     2221 aagttcttct cctttactca tatgtatatc tccttcttaa atctagagga tccccgggta
     2281 ccgagctcga attggggatc ttgaagttcc tattccgaag ttcctattct ctagaaagta
     2341 taggaacttc agagcgcttt tgaagctaat tcgagctcgg tacccgggct cgactgcatt
     2401 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgctcgaa
     2461 ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac
     2521 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     2581 tgttatgact gtttttttgt actcgagcag aaagtcaaaa gcctccgacc ggaggctttt
     2641 gacttgaggg ggatcgatcc cttatggctc tgcacccggc tccatcacca acaggtcgcg
     2701 cacgcgcttc actcggttgc ggatcgacac tgccagccca acaaagccgg ttgccgccgc
     2761 cgccaggatc gcgccgatga tgccggccac accggccatc gcccaccagg tcgccgcctt
     2821 ccggttccat tcctgctggt actgcttcgc aatgctggac ctcggctcac cataggctga
     2881 ccgctcgatg gcgtatgccg cttctcccct tggcgtaaaa cccagcgccg caggcggcat
     2941 tgccatgctg cccgccgctt tcccgaccac gacgcgcgca ccaggcttgc ggtccagacc
     3001 ttcggccacg gcgagctgcg caaggacata atcagccgcc gacttggctc cacgcgcctc
     3061 gatcagctct tgcactcgcg cgaaatcctt ggcctccacg gccgccatga atcgcgcacg
     3121 cggcgaaggc tccgcagggc cggcgtcgtg atcgccgccg agaatgccct tcaccaagtt
     3181 cgacgacacg aaaatcatgc tgacggctat caccatcatg cagacggatc gcacgaaccc
     3241 gcagaactca cccccgaaca cgagcacggc acccgcgacc actatgccaa gaatgcccaa
     3301 ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg
     3361 caggccgcca cccaggccgc cgccctcact gcccggcacc tggtcgctga atgtcgatgc
     3421 cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt
     3481 tactgccccg atcccggcaa tggcaaggac tgccagcgcc gcgatgagga agcgggtgcc
     3541 ccgcttcttc atcttcgcgc ctcgggcctc caggccgcct acctgggcga aaacatcggt
     3601 gtttgtggca ttcatacgga ctcctgttgg gccagctcgc gcacgggctg gcgggtcagc
     3661 ttggcttgaa gatcgccacg cattgcggcg atctgcttct cggcatcctt gcgcttctgc
     3721 acgccttcct gctggatgcg aataacgtcc tcgacggtct tgatgagcgt cgtctgaacc
     3781 tgcttgagcg tgtccacgtc gatcaccagg cgttggttct ccttcgccgt ctcgacggac
     3841 gtgcgatgca gcagggccgc attgcgcttc atcaggtcgt tggtggtgtc gtcgatggcc
     3901 gtggccagtt cgacggcgtt cttctgctcg ttgaggctca aggccagcat gaattgccgc
     3961 ttccacgccg gcacggtgat ttcgcggatg gtgtggaatt tatcgaccag catctggttg
     4021 ttggcctgga tcatgcggat ggtcggcagg ctctgcatgg ccgaatgttg caaggcgatc
     4081 aggtcgccga tgcgcttgtc caggttggca accatcgcat cgaggtcggc cagctcctgc
     4141 acgcggccag ggtcgttccc gacattgccg cgcagaccct cggcctgctc gcgcagctcg
     4201 gcaaggcgga ccttgccggc cgcgatgtgg acgccaagaa ggcggtgttc ctcgcgcacg
     4261 gctgcgaaca tttcgtcgag cgaggcattg cgctgcgcga tgccttgctg ggtggtctgc
     4321 acttcgctga ccaggtgttc gatctgctcg cgggtcgtgt cgaagcgcgc catgaagccc
     4381 gtcgaacgga cgcggaagcg gtcgatcagc gggccaatca ggggcaggcg ggaacggttg
     4441 tcggacaaag ggccgacgtt cagggaacgg gccttggcga caacctgggt cagtttctcg
     4501 cctgcttcgt ccaggtcgct gttgcgcacc tggtccagca ggctatcggc gtagcgggac
     4561 gtgtgctcgg ccacgtcgcg gccgaactcg gcaacggtct gcggactgcc gacctcgatc
     4621 cgctgcgcga ccgcatggac ttccggcacg tcgctttcct gcaagcccag ctcgcgcagg
     4681 gttgccgggg tcatgtcgaa ggcgacgata ggggccttgg cgtcgtgcgt cgttttcagt
     4741 gcgttcatag ggttctcccg ccgtgttatt ggttgatgcc ttccaggctc tgcgaaaggc
     4801 tccgcatgag cgcctggtga gctttggccg cctcggcgac cattgccgga ttcatgttct
     4861 tggtggtgat gagcgcgagg gtgtgctgac gccagacggg caccaggacg gatgccgttt
     4921 cagagaagcg gtccagcatg tccacggcct gcgcccgcgt gagcttcatc tgagtgacgc
     4981 tcatttcatg ggacgccatg agggttgcca ggttggcgag cttgcgcgcg aagcgttcgc
     5041 gcggcttgtc gaactcgatc acgccggcct tggccgcgcc ggcctcgggg ttctcgtcca
     5101 ggaactcgcg cccggcttga atgtaggctc tgagccggtc tacctcggcc tcatgcgtat
     5161 tgagcatgtc atccaaggcg cgcaacgtgt cccgcacgcg ctgcgctacg ccctcggctt
     5221 cgtccagcaa ctggtcgagc gtcttgcggg cgacctgata cctcacctgg cgttcaacct
     5281 cacggccaag catcttctcg aaccaggtag gcttttccgc gatcttgcgg gggtccgcgt
     5341 cggccagctt cgccacgatc tggctgattt tgtcggccag cgcggcaact gcgccgtgct
     5401 ccatcagatt cgacagctcg ttgagggaat ccgccccgtc gatgccggcc ccgtactcgc
     5461 caatcgtcgc cggcgacgcg aagagggcgg gcaaaacctc ccccttcaat cgcgccatgt
     5521 tcacgctttg ttcttccatg gtatatctcc ttcttaaagt taaacaaaat tattcggaac
     5581 ccagcatgat attccggaaa taccaactaa gtcaacggct gatggccaat tcggcttcct
     5641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagatc
     5761 gatcagcagt tcaacctgtt gatagtacgt actaagctct catgtttcac gtactaagct
     5821 ctcatgttta acgtactaag ctctcatgtt taacgaacta aaccctcatg gctaacgtac
     5881 taagctctca tggctaacgt actaagctct catgtttcac gtactaagct ctcatgtttg
     5941 aacaataaaa ttaatataaa tcagcaactt aaatagcctc taaggtttta agttttataa
     6001 gaaaaaaaag aatatataag gcttttaaag ctagctttta aggtttaacg gttgtggaca
     6061 acaagccagg gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttcagtg
     6121 acacaggaac acttaacggc tgacatgacg ctcagtggaa cgaaaactc
//
