LOCUS       40924_20496        7525 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Control vector pFRK1-LUC-NOS-At2g19190, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, 
            Boller T, Ausubel FM, Sheen J.
  TITLE     MAP kinase signalling cascade in Arabidopsis innate immunity
  JOURNAL   Nature 415 (6875), 977-983 (2002)
  PUBMED    11875555
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   He P, Shan L, Lin NC, Martin GB, Kemmerling B, Nurnberger T, Sheen 
            J.
  TITLE     Specific bacterial suppressors of MAMP signaling upstream of MAPKKK 
            in Arabidopsis innate immunity
  JOURNAL   Cell 125 (3), 563-575 (2006)
  PUBMED    16678099
REFERENCE   3  (bases 1 to 7525)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   4  (bases 1 to 7525)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2002"; volume: "415"; issue: "6875"; pages: "977-983"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Cell"; 
            date: "2006"; volume: "125"; issue: "3"; pages: "563-575"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2667..5461
                     /label=FRK1 promoter
                     /note="FRK1 promoter"
                     /regulatory_class="promoter"
     CDS             5462..7111
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      7269..7520
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      841 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2161 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2401 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2461 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     2521 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     2581 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
     2641 gcatgcctgc aggctctaga ggatccctga cagtgaactt cattgttcaa gcgaggtgag
     2701 tttcctattt ttcttcttct cctttcaatt aaaatttcag ggtttatgat ctctagggtt
     2761 taggttttat tttcttaaac taaaccctaa attctttttc ttcttctttt tcttgtaatt
     2821 tccagatgca ctgcgaacgc tagaggaggt gcgatatcga agaggaagcg ttatacagga
     2881 tcatgggacc aaaatatatc gtatagattc ggttcttgtt cttctatacc ttatgagagt
     2941 agaagttttt ctcttcaaga gaaaaaaaaa aaaaagaaaa aaaaaaaaga agagtagaaa
     3001 tttctctgtg ttttttttta ccaacaagac acaaatgaaa ctggtccaaa aggagtgtgt
     3061 ataatctctg tggagacata actaatacgt tgatgaattt caagaatact tggattatat
     3121 agattaaccc tgactccctt agatagagat cgaaatcggg tggtgatttc tgagaccata
     3181 caagttctga tcagattact actaagtaaa tcttaattat tagactgttt ttaatggatt
     3241 tttctgctct aatgataacg taatgagatt tttttttttt ttttgtatgt ttggcttgca
     3301 gacccgggta atgagaagtt tggtaagagc aaaaggcact aatctcacgt aagaaaacac
     3361 ttttttcatc aaccatgtat ataatcatgt cggtttacat aaaccgtatc gtctattcaa
     3421 gaatttagtt ttgtataatt ataatatttt tttcagacta cttttcaatt aagcatcttt
     3481 ttcttggatt tttttttcac aaagggagag ctttaacttt ttgcattaac ttatatattt
     3541 taaattatac atgcatgcat accgacttat ataaatcata tggtcaatat gagacttttg
     3601 atttatatta tttgtcaact aagcatcttt tcagatgagg ttcatgcacc tttgttagaa
     3661 ttatcggacc agaagatcac atcaacgttt accaaatcaa caaaaaaatc caatccgtcc
     3721 aaaaaatttg gaaactgttt gaaagattcg aaatgttgga gcaaggatac tcagttccaa
     3781 tctctgagca gaatctgata tgactcatct actcataaga ctttgcgaga tagaccggta
     3841 caaaaccgtt tccaagggtt cataatatat ggattaatgt gagttattgt ggacgttgtg
     3901 gttgtagaag ccgcggtagt cgtggaaaca ctaattagtt atctcctgta agctattttt
     3961 attttttttc ctcacttcct cttcctcgca gctatgtata attttggttg gttatctcca
     4021 aaacttttta tggtcaaaca attttgagaa agtaatttgg aaagaaaaaa ggtttaagaa
     4081 tatcttttta tcgtcaagtc ctcctccaaa ttacttcctt aaaactgttt gaccataaaa
     4141 agtcatagca aggtattagt tattctaatt taaattaaac gcctttttat caacaacaaa
     4201 aaaaagaaga agatttgata tgttgaaaag tattagggac gcttattagg gcagtattcc
     4261 tagttattgc attttctttc ggtcattcga ccttaggatc atacctcaat ctgtatgact
     4321 gtattcgcca tgtgaattcc aatattacat agtgaccaaa tctgatatcc aactaaaagt
     4381 cgatctttga tctaaacgaa atgacaacta tttggttagt gattgcaggt tggaaagatt
     4441 taccttctag acctgtctta cgaagctagt attctaaagt aatcttcata aaccgaattc
     4501 agaaacaaaa aaagaaaagg agtccaaaat tgtatgatca tacattaata tcagaatagt
     4561 ctcttttgtt aaataaatat ctgaagaata tatatctctt tgattatttt gtggatggca
     4621 atgaaactaa gaatatatat tcattgactt agaagtcgac aaaaaaaaat aaaaaaatta
     4681 ttgacttaat tactagttga ccaatatata tattattaaa agaacatatt gtatcgttga
     4741 aagcggatca tcgggtttta aaagaaaaac acatcgttga aacttgaaag tgatgactaa
     4801 taaaaagatc taaacgtgtc cggtcaccta ccaatgtggt tttgcaaatt attgtcaagt
     4861 accttgacta tattaaataa aaaaattcac cgtaacacat tgatattcaa ctgattccta
     4921 aaaaaatata caaactattg ggagttgtga gattttttat atcagtgttg gtctctttac
     4981 atttgtgatg tggtgttata gcatatatag taataaactc aaaaggaaat tagatgtgtt
     5041 ttgaccattt attaaaatga accttttctt gtcaaacatt tgaaaaatac tagttttttt
     5101 ttttggcaac gttgtaaata atagttaaaa atagatttta agtctcgttt ttttatgcat
     5161 atagtttcat tcgctttatt agactcaaat atacttttaa ttaaattttg cagagaatta
     5221 aaggtaatca tttgccaagg aaaaaccatg caaatatgca ataagtagaa ataatgttaa
     5281 tgagagtaag cgttgacata tattacgtcc tggtccgaac attcttaaag ttgcgtaaca
     5341 ctaataaccc tagaagatgg ttggttgact atcaacatct tattgaccaa atgttttttt
     5401 ttttttaatt ataaaacagt tgctcattgc tctagcccag agaaagcagc tcaataactc
     5461 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg
     5521 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
     5581 tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc
     5641 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
     5701 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
     5761 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat
     5821 ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca
     5881 aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg
     5941 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
     6001 ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg
     6061 atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc
     6121 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
     6181 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
     6241 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta
     6301 caaaattcaa agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct
     6361 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc
     6421 gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata
     6481 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     6541 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     6601 aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg
     6661 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     6721 tggagacata gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc
     6781 tttaattaaa tacaaaggat atcaggtggc ccccgctgaa ttggaatcga tattgttaca
     6841 acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact
     6901 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     6961 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     7021 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     7081 aaaggccaag aagggcggaa agtccaaatt gtaaaatgta actgtattca gcgatgacga
     7141 aattcttagc tattgtaata ttatatgcaa attgatgaat ggtaattttg taattgtggg
     7201 tcactgtact attttaacga ataataaaat caggtatagg taactaaaaa ggaattcgag
     7261 ctcgaattga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     7321 gtcttgcgat gattatcata taatttctgt tgattacgtt aagcatgtaa taattaacat
     7381 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     7441 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     7501 gtcatctatg ttactagatc tctag
//
