LOCUS       Exported                5764 bp DNA     circular SYN 20-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5764)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          998..1938
                     /plasmid="pFR-Luc"
                     /lab_host="Escherichia coli K12"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:75696"
                     /organism="Cloning vector pFR-Luc"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             137..997
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      998..1938
                     /label=Col EI origin
                     /note="Col EI origin"
     misc_feature    1942..2082
                     /label=bom
                     /note="basis of mobility region from pBR322"
     polyA_signal    2321..2455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2549..2683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2705..2799
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             2880..4532
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          4663..4728
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5303..5437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1801 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1861 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1921 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     1981 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2041 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2101 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2161 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2221 caggatcagc catatcacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2281 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt aacttgttta ttgcagctta
     2341 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2401 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca
     2461 tatcacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     2521 gaaacataaa atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2581 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2641 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccaagctt gcatgcctgc
     2701 aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
     2761 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta gagggtatat
     2821 aatggatccc cgggtaccga gctcgaattc cagcttggca ttccggtact gttggtaaaa
     2881 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta gaggatggaa
     2941 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
     3001 cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg
     3061 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
     3121 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
     3181 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgaacattt
     3241 cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
     3301 aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat taccagggat
     3361 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
     3421 ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat tcctctggat
     3481 ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc agattctcgc
     3541 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     3601 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     3661 gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt caggattaca
     3721 aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa agcactctga
     3781 ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca cctctttcga
     3841 aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga caaggatatg
     3901 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     3961 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     4021 cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt atgtccggtt
     4081 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     4141 gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc ttgaagtctt
     4201 taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata ttgttacaac
     4261 accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc
     4321 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     4381 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
     4441 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
     4501 aggccaagaa gggcggaaag tccaaattgt aaaatgtaac tgtattcagc gatgacgaaa
     4561 ttcttagcta ttgtaatact ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg
     4621 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     4681 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     4741 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     4801 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     4861 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     4921 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     4981 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     5041 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     5101 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     5161 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     5221 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     5281 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     5341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5401 gtccaaactc atcaatgtat cttatcatgt ctggatcccc aggaagctcc tctgtgtcct
     5461 cataaaccct aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc
     5521 tctgtgtcct cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca
     5581 cagaccgctt tctaagggta attttaaaat atctgggaag tcccttccac tgctgtgttc
     5641 cagaagtgtt ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcagctt
     5701 tgcacaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5761 ctta
//
