LOCUS       .                       5794 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(182..770)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(944..1801)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(1802..1906)
                     /label="AmpR promoter"
     CDS             2235..2348
                     /gene="rpmJ"
                     /label="rpmJ"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     CDS             2640..3356
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     oriT            4405..4492
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt cttcaagaat tcgagctcgg tacccgggga tcccggagtt
     2041 catgcgtgat gcaatgaaag tgccgttcta cttcggtggg acctcactgc ttatcgttgt
     2101 tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca gtcagtatga
     2161 gtctgcattg aagaaggcga acctgaaagg ctacggccgt taattggtcg cctgagaagt
     2221 tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt aactgcaaaa
     2281 tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag cataaacagc
     2341 gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg gctagattag
     2401 ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa cgggcttttc
     2461 agcatggtac gtacatatta aatagtagga gtgcatagtg gcccgtatag caggcattaa
     2521 cattcctgat cagaaacacg ccgtgatcgc gttaacttcg atctacggtg tcggcaagac
     2581 ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa aatgttaaga tcctctagaa
     2641 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2701 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2761 gcaagctgac cctgaagttc atctccacca ccggcaagct gcccgtgccc tggcccaccc
     2821 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     2881 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2941 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3001 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3061 agctggagta caactacaac tgccacaacg tctatatcat ggccgacaag cagaagaacg
     3121 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3181 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3241 acctgagcac ctgctccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3301 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagacta
     3361 gtggtggttc aggtggtggt ggttcaggtg gtggtggttc aggtggagga ggatcaggag
     3421 gaggaggatc aggaggagga ggatcaggag gagaattcgc tcaagagttt gtgaactgca
     3481 aaatccagcc tgggaaggtg gttgtgttca tcaagcccac ctgcccgtac tgcaggaggg
     3541 cccaagagat cctcagtcaa ttgcccatca aacaagggct tctggaattt gtcgatatca
     3601 cagccaccaa ccacactaac gagattcaag attatttgca acagctcacg ggagcaagaa
     3661 cggtgcctcg agtctttatt ggtaaagatt gtataggcgg atgcagtgat ctagtctctt
     3721 tgcaacagag tggggaactg ctgacgcggc taaagcagat tggagctctg cagggtacct
     3781 ctagaggatc tttgtgaaag ctttaatgcg gtagtttatc acagttaaat tgctaacgca
     3841 gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc
     3901 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg
     3961 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctttta gccgctttag
     4021 cggcctttcc ccctacccga agggtggggg cgcgtgtgca gccccgcagg gcctgtctcg
     4081 gtcgatcatt cagcccggct catccttctg gcgtggcggc agaccgaaca aggcgcggtc
     4141 gtggtcgcgt tcaaggtacg catccattgc cgccatgagc cgatcctccg gccactcgct
     4201 gctgttcacc ttggccaaaa tcatggcccc caccagcacc ttgcgccttg tttcgttctt
     4261 gcgctcttgc tgctgttccc ttgcccgcac ccgctgaatt tcggcattga ttcgcgctcg
     4321 ttgttcttcg agcttggcca gccgatccgc cgccttgttg ctccccttaa ccatcttgac
     4381 accccattgt taatgtgctg tctcgtaggc tatcatggag gcacagcggc ggcaatcccg
     4441 accctacttt gtaggggagg gggcacttac cggtttctct tcgagaaact ggcctaacgg
     4501 ccacccttcg ggcggtgcgc tctccgaggg ccattgcatg gagccgaaaa gcaaaagcaa
     4561 cagcgaggca gcatggcgat ttatcacctt acggcgaaaa ccggcagcag gtcgggcggc
     4621 caatcggcca gggccaaggc cgactacatc cagcgcgaag gcaagtatgc ccgcgacatg
     4681 gatgaagtct tgcacgccga atccgggcac atgccggagt tcgtcgagcg gcccgccgac
     4741 tactgggatg ctgccgacct gtatgaacgc gccaatgggc ggctgttcaa ggaggtcgaa
     4801 tttgccctgc cggtcgagct gaccctcgac cagcagaagg cgctggcgtc cgagttcgcc
     4861 cagcacctga ccggtgccga gcgcctgccg tatacgctgg ccatccatgc cggtggcggc
     4921 gagaacccgc actgccacct gatgatctcc gagcggatca atgacggcat cgagcggccc
     4981 gccgctcagt ggttcaagcg gtacaacggc aagaccccgg agaagggcgg ggcacagaag
     5041 accgaagcgc tcaagcccaa ggcatggctt gagcagaccc gcgaggcatg ggccgaccat
     5101 gccaaccggg cattagagcg ggctggccac gacgcccgca ttgaccacag aacacttgag
     5161 gcgcagggca tcgagcgcct gcccggtgtt cacctggggc cgaacgtggt ggagatggaa
     5221 ggccggggca tccgcaccga ccgggcagac gtggccctga acatcgacac cgccaacgcc
     5281 cagatcatcg acttacagga ataccgggag gcaatagacc atgaacgcaa tcgacagagt
     5341 gaagaaatcc agaggcatca acgagttagc ggagcagatc gaaccgctgg cccagagcat
     5401 ggcgacactg gccgacgaag cccggcaggt catgagccag acccagcagg ccagcgaggc
     5461 gcaggcggcg gagtggctga aagcccagcg ccagacaggg gcggcatggg tggagctggc
     5521 caaagagttg cgggaggtag ccgccgaggt gagcagcgcc gcgcagagcg cccggagcgc
     5581 gtcgcggggg tggcactgga agctatggct aaccgtgatg ctggcttcca tgatgcctac
     5641 ggtggtgctg ctgatcgcat cgttgctctt gctcgacctg acgccactga caaccgagga
     5701 cggctcgatc tggctgcgct tggtggcccg atgaagaacg acaggacttt gcaggccata
     5761 ggccgacagc tcaaggccat gggctgtgag cgct
//
