LOCUS       V014138                 5356 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014138
VERSION     V014138
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(1303..1390)
                     /direction=LEFT
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
     CDS             complement(2023..2697)
                     /label="mRFP1"
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     CDS             complement(3009..3122)
                     /gene="rpmJ"
                     /label="Large ribosomal subunit protein bL36A"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     promoter        3451..3555
                     /label="AmpR promoter"
     CDS             3556..4413
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4587..5175
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agcgctcaca gcccatggcc ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct
       61 tcatcgggcc accaagcgca gccagatcga gccgtcctcg gttgtcagtg gcgtcaggtc
      121 gagcaagagc aacgatgcga tcagcagcac caccgtaggc atcatggaag ccagcatcac
      181 ggttagccat agcttccagt gccacccccg cgacgcgctc cgggcgctct gcgcggcgct
      241 gctcacctcg gcggctacct cccgcaactc tttggccagc tccacccatg ccgcccctgt
      301 ctggcgctgg gctttcagcc actccgccgc ctgcgcctcg ctggcctgct gggtctggct
      361 catgacctgc cgggcttcgt cggccagtgt cgccatgctc tgggccagcg gttcgatctg
      421 ctccgctaac tcgttgatgc ctctggattt cttcactctg tcgattgcgt tcatggtcta
      481 ttgcctcccg gtattcctgt aagtcgatga tctgggcgtt ggcggtgtcg atgttcaggg
      541 ccacgtctgc ccggtcggtg cggatgcccc ggccttccat ctccaccacg ttcggcccca
      601 ggtgaacacc gggcaggcgc tcgatgccct gcgcctcaag tgttctgtgg tcaatgcggg
      661 cgtcgtggcc agcccgctct aatgcccggt tggcatggtc ggcccatgcc tcgcgggtct
      721 gctcaagcca tgccttgggc ttgagcgctt cggtcttctg tgccccgccc ttctccgggg
      781 tcttgccgtt gtaccgcttg aaccactgag cggcgggccg ctcgatgccg tcattgatcc
      841 gctcggagat catcaggtgg cagtgcgggt tctcgccgcc accggcatgg atggccagcg
      901 tatacggcag gcgctcggca ccggtcaggt gctgggcgaa ctcggacgcc agcgccttct
      961 gctggtcgag ggtcagctcg accggcaggg caaattcgac ctccttgaac agccgcccat
     1021 tggcgcgttc atacaggtcg gcagcatccc agtagtcggc gggccgctcg acgaactccg
     1081 gcatgtgccc ggattcggcg tgcaagactt catccatgtc gcgggcatac ttgccttcgc
     1141 gctggatgta gtcggccttg gccctggccg attggccgcc cgacctgctg ccggttttcg
     1201 ccgtaaggtg ataaatcgcc atgctgcctc gctgttgctt ttgcttttcg gctccatgca
     1261 atggccctcg gagagcgcac cgcccgaagg gtggccgtta ggccagtttc tcgaagagaa
     1321 accggtaagt gccccctccc ctacaaagta gggtcgggat tgccgccgct gtgcctccat
     1381 gatagcctac gagacagcac attaacaatg gggtgtcaag atggttaagg ggagcaacaa
     1441 ggcggcggat cggctggcca agctcgaaga acaacgagcg cgaatcaatg ccgaaattca
     1501 gcgggtgcgg gcaagggaac agcagcaaga gcgcaagaac gaaacaaggc gcaaggtgct
     1561 ggtgggggcc atgattttgg ccaaggtgaa cagcagcgag tggccggagg atcggctcat
     1621 ggcggcaatg gatgcgtacc ttgaacgcga ccacgaccgc gccttgttcg gtctgccgcc
     1681 acgccagaag gatgagccgg gctgaatgat cgaccgagac aggccctgcg gggctgcaca
     1741 cgcgccccca cccttcgggt agggggaaag gccgctaaag cggctaaaag cgctagcagc
     1801 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
     1861 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     1921 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     1981 ctaccgcatt aaagcttgca tgcctgcagg tctggacatt taagcaccgg tggagtgacg
     2041 accttcagca cgttcgtact gttcaacgat ggtgtagtct tcgttgtggg aggtgatgtc
     2101 cagtttgatg tcggttttgt aagcacccgg cagctgaacc ggttttttag ccatgtaggt
     2161 ggttttaact tcagcgtcgt agtgaccacc gtctttcagt ttcagacgca ttttgatttc
     2221 acctttcaga gcaccgtctt ccgggtacat acgttcggtg gaagcttccc aacccatggt
     2281 ttttttctgc ataaccggac catcggacgg gaagttggta ccacgcagtt taactttgta
     2341 gatgaactca ccgtcttgca gggaggagtc ctgggtaacg gtaacaacac caccgtcttc
     2401 gaagttcata acacgttccc atttgaaacc ttccgggaag gacagtttca ggtagtccgg
     2461 gatgtcagcc gggtgtttaa cgtaagcttt ggaaccgtac tggaactgcg gggacaggat
     2521 gtcccaagcg aacggcagcg gaccaccttt ggtaactttc agtttagcgg tctgggtacc
     2581 ttcgtacgga cgaccttcac cttcaccttc gatttcgaac tcgtgaccgt taacggaacc
     2641 ttccatacga actttgaaac gcatgaactc tttgataacg tcttcggagg aagccatatg
     2701 tatatctcct tcttaaatct agaggatctt aacattttca gcgatacccg ctgcagccag
     2761 gatggcttta gaacgggtct tgccgacacc gtagatcgaa gttaacgcga tcacggcgtg
     2821 tttctgatca ggaatgttaa tgcctgctat acgggccact atgcactcct actatttaat
     2881 atgtacgtac catgctgaaa agcccgtttt caggatactc aaatggaaac gtacagacat
     2941 acaaaagatt ggctggctaa tctagccagc tcaacccaac tttgcaagaa aaatatgcga
     3001 aaaaatcagc cttggcgctg tttatgcttc ggctcggcac tgcaaatcac acggatgaca
     3061 ccatcacgct taacgatttt gcagttacgg cataatttct tgacggaagc acgaactttc
     3121 atttttactc tccgtaactt ctcaggcgac caattaacgg ccgtagcctt tcaggttcgc
     3181 cttcttcaat gcagactcat actgactgga catcatcaga gtttgcactt gagccataaa
     3241 gtccataatc acgacaacaa cgataagcag tgaggtccca ccgaagtaga acggcacttt
     3301 cattgcatca cgcatgaact ccgggatccc cgggtaccga gctcgaattc ttgaagacga
     3361 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3421 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3481 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3541 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3601 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3661 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3721 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3781 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     3841 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3901 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3961 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4021 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4081 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4141 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4201 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4261 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4321 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4381 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4441 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4501 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4561 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4621 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4681 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     4741 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4801 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4861 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4921 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4981 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5041 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5101 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5161 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5221 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5281 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5341 agcgaggaag cggaag
//
