LOCUS       V014172                 5397 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014172
VERSION     V014172
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(944..1801)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1802..1906)
                     /label="AmpR promoter"
     CDS             2235..2348
                     /gene="rpmJ"
                     /label="Large ribosomal subunit protein bL36A"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     CDS             2660..3376
                     /label="EYFP"
                     /note="enhanced YFP"
     oriT            4008..4095
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt cttcaagaat tcgagctcgg tacccgggga tcccggagtt
     2041 catgcgtgat gcaatgaaag tgccgttcta cttcggtggg acctcactgc ttatcgttgt
     2101 tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca gtcagtatga
     2161 gtctgcattg aagaaggcga acctgaaagg ctacggccgt taattggtcg cctgagaagt
     2221 tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt aactgcaaaa
     2281 tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag cataaacagc
     2341 gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg gctagattag
     2401 ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa cgggcttttc
     2461 agcatggtac gtacatatta aatagtagga gtgcatagtg gcccgtatag caggcattaa
     2521 cattcctgat cagaaacacg ccgtgatcgc gttaacttcg atctacggtg tcggcaagac
     2581 ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa aatgttaaga tcctctagat
     2641 ttaagaagga gatatacata tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2701 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2761 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2821 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg
     2881 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2941 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3001 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3061 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3121 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3181 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3241 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     3301 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3361 ggacgagctg tacaagtaat gtccagacct gcaggcatgc aagctttaat gcggtagttt
     3421 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat
     3481 cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact
     3541 gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct
     3601 gctagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt
     3661 gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc
     3721 ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg
     3781 agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc
     3841 accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg cacccgctga
     3901 atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg
     3961 ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg
     4021 gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggggcact taccggtttc
     4081 tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc
     4141 atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga
     4201 aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg
     4261 aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg
     4321 agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg
     4381 ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga
     4441 aggcgctggc gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc
     4501 tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga
     4561 tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc
     4621 cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga
     4681 cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc
     4741 gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg
     4801 ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc
     4861 tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag
     4921 accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag
     4981 atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc
     5041 cagacccagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca
     5101 ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc
     5161 gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg
     5221 atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac
     5281 ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga
     5341 acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgct
//
