LOCUS       62056_10940        4047 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(1303..1390)
                     /direction=LEFT
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     promoter        2142..2246
                     /label=AmpR promoter
     CDS             2247..3104
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3278..3866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgctcaca gcccatggcc ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct
       61 tcatcgggcc accaagcgca gccagatcga gccgtcctcg gttgtcagtg gcgtcaggtc
      121 gagcaagagc aacgatgcga tcagcagcac caccgtaggc atcatggaag ccagcatcac
      181 ggttagccat agcttccagt gccacccccg cgacgcgctc cgggcgctct gcgcggcgct
      241 gctcacctcg gcggctacct cccgcaactc tttggccagc tccacccatg ccgcccctgt
      301 ctggcgctgg gctttcagcc actccgccgc ctgcgcctcg ctggcctgct gggtctggct
      361 catgacctgc cgggcttcgt cggccagtgt cgccatgctc tgggccagcg gttcgatctg
      421 ctccgctaac tcgttgatgc ctctggattt cttcactctg tcgattgcgt tcatggtcta
      481 ttgcctcccg gtattcctgt aagtcgatga tctgggcgtt ggcggtgtcg atgttcaggg
      541 ccacgtctgc ccggtcggtg cggatgcccc ggccttccat ctccaccacg ttcggcccca
      601 ggtgaacacc gggcaggcgc tcgatgccct gcgcctcaag tgttctgtgg tcaatgcggg
      661 cgtcgtggcc agcccgctct aatgcccggt tggcatggtc ggcccatgcc tcgcgggtct
      721 gctcaagcca tgccttgggc ttgagcgctt cggtcttctg tgccccgccc ttctccgggg
      781 tcttgccgtt gtaccgcttg aaccactgag cggcgggccg ctcgatgccg tcattgatcc
      841 gctcggagat catcaggtgg cagtgcgggt tctcgccgcc accggcatgg atggccagcg
      901 tatacggcag gcgctcggca ccggtcaggt gctgggcgaa ctcggacgcc agcgccttct
      961 gctggtcgag ggtcagctcg accggcaggg caaattcgac ctccttgaac agccgcccat
     1021 tggcgcgttc atacaggtcg gcagcatccc agtagtcggc gggccgctcg acgaactccg
     1081 gcatgtgccc ggattcggcg tgcaagactt catccatgtc gcgggcatac ttgccttcgc
     1141 gctggatgta gtcggccttg gccctggccg attggccgcc cgacctgctg ccggttttcg
     1201 ccgtaaggtg ataaatcgcc atgctgcctc gctgttgctt ttgcttttcg gctccatgca
     1261 atggccctcg gagagcgcac cgcccgaagg gtggccgtta ggccagtttc tcgaagagaa
     1321 accggtaagt gccccctccc ctacaaagta gggtcgggat tgccgccgct gtgcctccat
     1381 gatagcctac gagacagcac attaacaatg gggtgtcaag atggttaagg ggagcaacaa
     1441 ggcggcggat cggctggcca agctcgaaga acaacgagcg cgaatcaatg ccgaaattca
     1501 gcgggtgcgg gcaagggaac agcagcaaga gcgcaagaac gaaacaaggc gcaaggtgct
     1561 ggtgggggcc atgattttgg ccaaggtgaa cagcagcgag tggccggagg atcggctcat
     1621 ggcggcaatg gatgcgtacc ttgaacgcga ccacgaccgc gccttgttcg gtctgccgcc
     1681 acgccagaag gatgagccgg gctgaatgat cgaccgagac aggccctgcg gggctgcaca
     1741 cgcgccccca cccttcgggt agggggaaag gccgctaaag cggctaaaag cgctagcagc
     1801 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
     1861 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     1921 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     1981 ctaccgcatt aaagcttgca tgcctgcagg tctggacatc ccgggtaccg agctcgaatt
     2041 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2101 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2161 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2221 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2281 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2341 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2401 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2461 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     2521 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2581 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2641 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2701 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2761 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     2821 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2881 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2941 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3001 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3061 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3121 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3421 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3661 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3841 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3961 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4021 gcgagtcagt gagcgaggaa gcggaag
//
