LOCUS       V014327                 5373 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014327
VERSION     V014327
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             226..339
                     /gene="rpmJ"
                     /label="Large ribosomal subunit protein bL36A"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     CDS             651..1358
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     oriT            1975..2062
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      complement(3546..4134)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4308..5165)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5166..5270)
                     /label="AmpR promoter"
ORIGIN
        1 ttcgagctcg gtacccgggg atcccggagt tcatgcgtga tgcaatgaaa gtgccgttct
       61 acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt atggctcaag
      121 tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg aacctgaaag
      181 gctacggccg ttaattggtc gcctgagaag ttacggagag taaaaatgaa agttcgtgct
      241 tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt catccgtgtg
      301 atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg catatttttc
      361 ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg tctgtacgtt
      421 tccatttgag tatcctgaaa acgggctttt cagcatggta cgtacatatt aaatagtagg
      481 agtgcatagt ggcccgtata gcaggcatta acattcctga tcagaaacac gccgtgatcg
      541 cgttaacttc gatctacggt gtcggcaaga cccgttctaa agccatcctg gctgcagcgg
      601 gtatcgctga aaatgttaag atcctctaga tttaagaagg agatatacat atggtgagca
      661 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      721 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      781 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      841 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      901 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      961 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     1021 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1081 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1141 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1201 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1261 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1321 gccgccactc caccggcggc atggacgagc tgtacaagta agcatgcaag ctttaatgcg
     1381 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     1441 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     1501 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     1561 gcgtgctgct agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg
     1621 cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct catccttctg
     1681 gcgtggcggc agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg catccattgc
     1741 cgccatgagc cgatcctccg gccactcgct gctgttcacc ttggccaaaa tcatggcccc
     1801 caccagcacc ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc ttgcccgcac
     1861 ccgctgaatt tcggcattga ttcgcgctcg ttgttcttcg agcttggcca gccgatccgc
     1921 cgccttgttg ctccccttaa ccatcttgac accccattgt taatgtgctg tctcgtaggc
     1981 tatcatggag gcacagcggc ggcaatcccg accctacttt gtaggggagg gcgcacttac
     2041 cggtttctct tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg
     2101 ccattgcatg gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt
     2161 acggcgaaaa ccggcagcag gtcgggcggc caatcggcca gggccaaggc cgactacatc
     2221 cagcgcgaag gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga atccgggcac
     2281 atgccggagt tcgtcgagcg gcccgccgac tactgggatg ctgccgacct gtatgaacgc
     2341 gccaatgggc ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac
     2401 cagcagaagg cgctggcgtc cgagttcgcc cagcacctga ccggtgccga gcgcctgccg
     2461 tatacgctgg ccatccatgc cggtggcggc gagaacccgc actgccacct gatgatctcc
     2521 gagcggatca atgacggcat cgagcggccc gccgctcagt ggttcaagcg gtacaacggc
     2581 aagaccccgg agaagggcgg ggcacagaag accgaagcgc tcaagcccaa ggcatggctt
     2641 gagcagaccc gcgaggcatg ggccgaccat gccaaccggg cattagagcg ggctggccac
     2701 gacgcccgca ttgaccacag aacacttgag gcgcagggca tcgagcgcct gcccggtgtt
     2761 cacctggggc cgaacgtggt ggagatggaa ggccggggca tccgcaccga ccgggcagac
     2821 gtggccctga acatcgacac cgccaacgcc cagatcatcg acttacagga ataccgggag
     2881 gcaatagacc atgaacgcaa tcgacagagt gaagaaatcc agaggcatca acgagttagc
     2941 ggagcagatc gaaccgctgg cccagagcat ggcgacactg gccgacgaag cccggcaggt
     3001 catgagccag acccagcagg ccagcgaggc gcaggcggcg gagtggctga aagcccagcg
     3061 ccagacaggg gcggcatggg tggagctggc caaagagttg cgggaggtag ccgccgaggt
     3121 gagcagcgcc gcgcagagcg cccggagcgc gtcgcggggg tggcactgga agctatggct
     3181 aaccgtgatg ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt
     3241 gctcgacctg acgccactga caaccgagga cggctcgatc tggctgcgct tggtggcccg
     3301 atgaagaacg acaggacttt gcaggccata ggccgacagc tcaaggccat gggctgtgag
     3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3961 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     4021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4621 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4921 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5281 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     5341 aggcgtatca cgaggccctt tcgtcttcaa gaa
//
