LOCUS       V006205                 5818 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006205
VERSION     V006205
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Karig D, Martini KM, Lu T, DeLateur NA, Goldenfeld N, Weiss R.
  TITLE     Stochastic Turing patterns in a synthetic bacterial population
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   29891706
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Karig DK, Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Research and Development Department, Johns
            Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins
            Rd, Laurel, MD 20723, USA
REFERENCE   3  (bases 1 to 5818)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5818)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Research and Development Department, Johns Hopkins
            University Applied Physics Laboratory, 11100 Johns Hopkins Rd,
            Laurel, MD 20723, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(40..134)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(168..959)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(1106..1828)
                     /gene="rhlR"
                     /label="HTH-type quorum-sensing regulator RhlR"
                     /note="HTH-type quorum-sensing regulator RhlR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P54292"
     regulatory      complement(1829..1847)
                     /label="RBS G"
                     /note="RBS G"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1848..1897)
                     /regulatory_class="promoter"
     regulatory      complement(1861..1866)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(1885..1890)
                     /regulatory_class="minus_35_signal"
     protein_bind    1977..1993
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      2002..2018
                     /label="RBSH"
                     /note="RBSH"
                     /regulatory_class="ribosome_binding_site"
     CDS             2019..2729
                     /label="lambda repressor"
                     /note="phage lambda repressor"
     CDS             2736..2768
                     /label="ssrA tag (LVA)"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     RBS             2776..2787
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      2776..2784
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     CDS             2794..3507
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      3769..3855
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3945..5024)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(5025..5102)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     rep_origin      complement(5199..5744)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 agtcagcccc atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc
       61 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
      121 tatcaacagg agtccaagcg agctctcgaa ccccagagtc ccgctcagaa gaactcgtca
      181 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
      241 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
      301 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
      361 ttttccacca tgatattcgg caagcacgca tcgccatggg tcacgacgag atcctcgccg
      421 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
      481 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
      541 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
      601 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
      661 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
      721 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
      781 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
      841 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
      901 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
      961 cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt
     1021 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
     1081 ccagctggca attccgacgt cttcagatga ggcccagcgc cgcggcgtag gcggcagcca
     1141 gcgtcttgtt cggcgcgtcg aacttcttct ggatgttctt gtggtggaag ttcaccgtgc
     1201 tctcggaaat gctcaggatg atggcgattt ccccggagct cttgccgtcg gcggtccatt
     1261 gcaggatctc gcgttcgcga tggctcaggc agaccgggtt ggacatcagc atcggatgct
     1321 ccaggtcggt cagcttctgg gtcagcaact cgatcatgca acgcagccgc aggcgtattt
     1381 cctcgcgctc gaagctggag atgttctgct ggtcgcgcgc cacggaaagc acgctgagca
     1441 aattgttcgg cgcgcggaac ggcaaagtcg cgccgacaca gaggccccaa tcgcgagcct
     1501 cgttccagag catccggctc tggtcgaaca ggctgtcgct ccagaccacc atttccgagg
     1561 agcgcaggcc gttgaggatc gccggatcca cggccccgta gttctgcatc tggtatcgct
     1621 ccagccaggc cttgggatag gtgccatgga cctcggtctt cggccgggtg aagggaatcg
     1681 tatggcgcac gccataggcg tagtaatcga agcccaggcg ccgcacttcc ttttccagga
     1741 cggcgaacac gccctggctg tcgtggatcg gctgcatctc gctacgcaaa ccgtcccacc
     1801 acagcaaaaa gcctccgtca ttcctcatcg aggcctttcc tgtgtgacgt agctattgag
     1861 attatagtaa gagtgcttct atcatgtcaa tacactaacg gctcgcaagt atcggccgcc
     1921 cgtcctgtga aatctggcag ttaccgttag ctttcgaatt ggctaaaaag tgttaattgt
     1981 gagcggataa caatttacta gtcacacagg acggccggat gagcacaaaa aagaaaccat
     2041 taacacaaga gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa
     2101 atgaacttgg cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg
     2161 ttggtgcttt atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa
     2221 aaattctcaa agttagcatt gaagaattca gcccttcaat cgccagagag atctacgaga
     2281 tgtatgaagc ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt
     2341 ctcatgttca ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg
     2401 agagatgggt aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag
     2461 gtaattccat gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc
     2521 tcgttgaccc tgagcaggct gttgagcccg gggatttctg catagccaga cttgggggtg
     2581 atgagtttac cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa
     2641 acccacagta cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg
     2701 ctagtcagtg gcctgaagag acgtttggca ggcctgcagc aaacgacgaa aactacgctt
     2761 tagtagcttg atcgcaaaga ggagaaatta agcatgcgta aaggagaaga acttttcact
     2821 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     2881 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
     2941 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
     3001 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
     3061 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
     3121 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
     3181 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
     3241 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
     3301 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
     3361 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     3421 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     3481 acacatggca tggatgaact atacaaaagg cctgcagcaa acgacgaaaa ctacgcttta
     3541 gtagcttaat aagcttaatt agctgaccta ctagtcggcc gtacgggccc tttcgtctcg
     3601 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     3661 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     3721 gcgggtgtcg gggctggctt aactatgcgg catcagagca gtacgcatca aataaaacga
     3781 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3841 ctgagtagga caaatccgcc gccctagacc tagggatata ttccgcttcc tcgctcactg
     3901 actcgctacg ctcggtcgtt cgcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3961 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4021 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     4081 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     4141 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     4201 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     4261 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     4321 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     4381 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     4441 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     4501 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     4561 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     4621 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     4681 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     4741 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     4801 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     4861 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     4921 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     4981 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     5041 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca ttcgatggtg
     5101 tcaacgtagt acaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat
     5161 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct
     5221 gacaagcatc acgaaatctg acgcccaaat cagtggtggc gaaacccgac aggactataa
     5281 agataccagg cgtttccccc tggcggctcc cccgtgcgct ctcctgttcc tgcctttcgg
     5341 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
     5401 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     5461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc
     5521 accactggca gcagccactg gtaattggtt tagaggagtt agtcttgaag tcatgcgccg
     5581 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc
     5641 ggttcaaaga gctggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt
     5701 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta
     5761 atcagataaa atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctga
//
