LOCUS       V006207                 5080 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006207
VERSION     V006207
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Karig D, Martini KM, Lu T, DeLateur NA, Goldenfeld N, Weiss R.
  TITLE     Stochastic Turing patterns in a synthetic bacterial population
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   29891706
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Karig DK, Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Research and Development Department, Johns
            Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins
            Rd, Laurel, MD 20723, USA
REFERENCE   3  (bases 1 to 5080)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5080)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Research and Development Department, Johns Hopkins
            University Applied Physics Laboratory, 11100 Johns Hopkins Rd,
            Laurel, MD 20723, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(137..853)
                     /gene="lasR"
                     /label="Transcriptional activator protein LasR"
                     /note="Transcriptional activator protein LasR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P25084"
     CDS             1116..1718
                     /gene="lasI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Pseudomonas
                     aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
                     JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). Accession#:
                     P33883"
     CDS             1725..1757
                     /label="ssrA tag (LVA)"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     CDS             1842..2444
                     /gene="rhlI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Pseudomonas
                     aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
                     JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). Accession#:
                     P54291"
     CDS             2514..3188
                     /label="DsRed-Express"
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     terminator      3274..3360
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(3530..4118)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(4206..4300)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4324..4980)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(join(4981..5080,1..3))
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tcaacgtctc attttcgcca gatatcgacg tctaagaaac cattattatc atgacattaa
       61 cctataaaaa taggcgtatc acgaggccct ttcgtcttca tctcacgtca ttgcgtgcac
      121 ctgagaggca agatcagaga gtaataagac ccaaattaac ggccataatg gccgctacgc
      181 ggcgggaggt cacaccgaac ttccgccgaa tatttcccat atggaagttc acattggctt
      241 ccgagcagtt gcagataacc gatatctccc aactggtctt gccgatggcg caccactgca
      301 acacttcctt ctcccggctg gtcagaaccg ccggtttgct gaccggatgt tcgaaggcca
      361 gtccggcacc gctctgcagt gcgtagtcct tgagcatcca cagggtcggc aggaccgact
      421 ccatgaaacg gttggcctcg gcccggtttt ccgcttccac gctgaggctc agcgcgccga
      481 gttcgccgcg agcaccatgc agcggcatgg tcagcccata caccaggccg gcggccgagg
      541 cttcctcgaa gaactcgtgc tgctttcgcg tctggtagat ggacggtttc cagaaaatcg
      601 gcagtacgct ctgggtacag tgactgaccg tcgggtcgac ccgcgcgtag ccagcccggt
      661 cgtaatgctc gcgccaggcg gccgggtagt tgccgacgat gaaggcgttc tcgtagtcct
      721 ggctgtcctt aggcaacagg ccgaacagga tcttcgagaa tccaaggtcg ctcgccatct
      781 tctgcaggat ggcgctccac tccaattttc cacttgagcg ttccagctca agaaaaccgt
      841 caaccaaggc catgcttaat ttctcctctt taatgctctc ttccgggtcg ctatcaccgc
      901 cagatgtatt cgagcctagc aagggtccgg gttcaccgaa atctatctca tttgctagtt
      961 gcgaatcaag tgtgtacatg ggtccacact ttacttagta cttcatatac gatcccgatc
     1021 gcaactagca aatgagatag atttcggtga acccggaccc ttgctaggct cgaatacatc
     1081 tggcggtgat agttcgcaaa gaggagaaat taagcatgat cgtacaaatt ggtcggcgcg
     1141 aagagttcga taaaaaaccg ctgggcgaga tgcacaagtt gcgtgctcaa gtgttcaagg
     1201 agcgcaaagg ctgggacgtt agtgtcatcg acgagatgga aatcgatggt tatgacgcac
     1261 tcagtcctta ttacatgttg atccaggaag atactcctga agcccaggtt ttcggttgct
     1321 ggcgaattct cgataccact ggcccctaca tgctgaagaa caccttcccg gagcttctgc
     1381 acggcaagga agcgccttgc tcgccgcaca tctgggaact cagccgtttc gccatcaact
     1441 ctggacagaa aggctcgctg ggcttttccg actgtacgct ggaggcgatg cgcgcgctgg
     1501 cccgctacag cctgcagaac gacatccaga cgctggtgac ggtaaccacc gtaggcgtgg
     1561 agaagatgat gatccgtgcc ggcctggacg tatcgcgctt cggtccgcac ctgaagatcg
     1621 gcatcgagcg cgcggtggcc ttgcgcatcg aactcaatgc caagacccag atcgcgcttt
     1681 acgggggagt gctggtggaa cagcgactgg cggtttcacg cccggcggcg aatgatgaga
     1741 attatgcgct ggtggcgtaa taatggcgtc gatgccgaat ccgctgtaag gcattcatcg
     1801 gctactcgct tcgctcacca ggaaaagagg agaaattaag catgatcgaa ttgctctctg
     1861 aatcgctgga agggctttcc gccgccatga tcgccgagct gggacgctac cggcatcagg
     1921 tcttcatcga gaagctgggc tgggacgtgg tctccacctc cagggtccgc gaccaggaat
     1981 tcgaccagtt cgaccatccg caaacccgct acatcgtcgc catgagccgc cagggcatct
     2041 gcggttgcgc ccgcctgctg ccgacgaccg acgcctacct gctcaaggac gtcttcgcct
     2101 acctgtgcag cgaaaccccg ccgagcgatc cgtcggtctg ggagctttcg cgctacgccg
     2161 ccagcgcggc ggacgatccg cagctggcga tgaagatatt ctggtccagc ctgcaatgcg
     2221 cctggtacct gggcgccagt tcggtggtgg cggtgaccac cacggccatg gagcgctatt
     2281 tcgttcgcaa cggcgtgatc ctccagcgcc tcggcccgcc gcagaaggtc aagggcgaga
     2341 cgctggtcgc gatcagcttc ccggcctacc aggagcgcgg cctggagatg ctgctgcgct
     2401 accacccgga atggctgcag ggcgtaccgc tgtcgatggc ggtgtgacgc ccggcggcga
     2461 atgatgagaa ttatgcgctg gtggcgtatg ctattaaaga ggagaaatta agcatggcct
     2521 cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
     2581 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     2641 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     2701 cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca
     2761 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     2821 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg ctccttcatc tacaaggtga
     2881 agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actatgggct
     2941 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccaca
     3001 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc atctacatgg
     3061 ccaagaagcc cgtgcagctg cccggctact actacgtgga ctccaagctg gacatcacct
     3121 cccacaacga ggactacacc atcgtggagc agtacgagcg cgccgagggc cgccaccacc
     3181 tgttcctgta gcggccctac acgaaaacta cgctttagta gctaataagc ttaattagct
     3241 gacctactag tcggccgtct cgagtgtacg catcaaataa aacgaaaggc tcagtcgaaa
     3301 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     3361 ccgccgccct agacctaggg gatatattcc gcttcctcgc tcactgactc gctacagtaa
     3421 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3661 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3721 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3781 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3841 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3961 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4021 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4081 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4141 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgactagtg
     4201 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     4261 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     4321 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     4381 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     4441 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     4501 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     4561 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     4621 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     4681 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     4741 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     4801 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     4861 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     4921 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     4981 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     5041 tatttcatta tggtgaaagt tggaacctct tacgtgccga
//
