LOCUS       V006208                 3756 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006208
VERSION     V006208
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Karig D, Martini KM, Lu T, DeLateur NA, Goldenfeld N, Weiss R.
  TITLE     Stochastic Turing patterns in a synthetic bacterial population
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   29891706
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Karig DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Research and Development Department, Johns
            Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins
            Rd, Laurel, MD 20723, USA
REFERENCE   3  (bases 1 to 3756)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Research and Development Department, Johns Hopkins
            University Applied Physics Laboratory, 11100 Johns Hopkins Rd,
            Laurel, MD 20723, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(70..786)
                     /gene="lasR"
                     /label="Transcriptional activator protein LasR"
                     /note="Transcriptional activator protein LasR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P25084"
     regulatory      complement(796..804)
                     /label="RBS II"
                     /note="RBS II"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(816..871)
                     /regulatory_class="promoter"
     regulatory      complement(825..830)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(848..853)
                     /regulatory_class="minus_35_signal"
     regulatory      907..963
                     /note="prokaryotic; qscrsaL"
                     /regulatory_class="promoter"
     misc_feature    907..926
                     /label="las box - qscrsaL"
                     /note="las box - qscrsaL"
     RBS             1001..1012
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      1001..1009
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     CDS             1019..1732
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      1823..1909
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2078..2623)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2737..2831)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2865..3656)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaaccttac cagagggcgc cccagctggc aattccgacg tcattgcgtg cacctgagag
       61 gcaagatcag agagtaataa gacccaaatt aacggccata atggccgcta cgcggcggga
      121 ggtcacaccg aacttccgcc gaatatttcc catatggaag ttcacattgg cttccgagca
      181 gttgcagata accgatatct cccaactggt cttgccgatg gcgcaccact gcaacacttc
      241 cttctcccgg ctggtcagaa ccaccggttt gctgaccgga tgttcgaagg ccagtccggc
      301 accgctctgc agtgcgtagt ccttgagcat ccacagggtc ggcaggaccg actccatgaa
      361 acggttggcc tcggcccggt tttccgcttc cacgctgagg ctcagcgcgc cgagttcgcc
      421 gcgagcacca tgcagcggca tggtcagccc atacaccagg ccggcggccg aggcttcctc
      481 gaagaactcg tgctgctttc gcgtctggta gatggacggt tcccagaaaa tcggcagtac
      541 gctctgggta cagtgactga ccgtcgggtc gacccgcgcg tagccagccc ggtcgtaatg
      601 ctcgcgccag gcggccgggt agttgccgac gatgaaggcg ttctcgtagt cctggctgtc
      661 cttaggcaac aggccgaaca ggatcttcga gaatccaagg tcgctcgcca tcttctgcag
      721 gatggcgctc cactccaatt ttccacttga gcgttccagc tcaagaaaac cgtcaaccaa
      781 ggccatgctt aatttcgcct ctttaatgct ctcttccggg cgctatcatg ccataccgcg
      841 aaaggttttg caccattcga tggtgtcaac gtaatctaga gctagcacga caggtttccc
      901 gatcgcaact agcaaatgag atagatttcg gtgaacccgg acccttgcta ggctcgaaga
      961 aacgtactcg actctagagg atccccgggt acctagaatt aaagaggaga aattaagcat
     1021 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1081 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1141 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     1201 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     1261 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     1321 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     1381 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     1441 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     1501 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     1561 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     1621 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agggaccaca tggtccttct
     1681 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc
     1741 agcaaacgac gaaaactacg ctttagtagc ttaataagct taattagctg acctactagt
     1801 cggccgtctc gagtagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1861 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
     1921 gacctaggga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     1981 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
     2041 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     2101 acaagcatca cgaaatctga cgcccaaatc agtggtggcg aaacccgaca ggactataaa
     2161 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
     2221 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
     2281 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     2341 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
     2401 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
     2461 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
     2521 gttcaaagag ctggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
     2581 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
     2641 tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt
     2701 cagccccata cgatataagt tgttactagt gcttggattc tcaccaataa aaaacgcccg
     2761 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     2821 caacaggagt ccaagcgagc tctcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     2881 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     2941 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     3001 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     3061 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     3121 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     3181 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     3241 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     3301 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     3361 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     3421 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     3481 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     3541 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     3601 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     3661 aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc
     3721 ggcaagaaag ccatccagtt tactttgcag ggcttc
//
