LOCUS       V006209                 3866 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006209
VERSION     V006209
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Karig D, Martini KM, Lu T, DeLateur NA, Goldenfeld N, Weiss R.
  TITLE     Stochastic Turing patterns in a synthetic bacterial population
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   29891706
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Karig DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Research and Development Department, Johns
            Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins
            Rd, Laurel, MD 20723, USA
REFERENCE   3  (bases 1 to 3866)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Research and Development Department, Johns Hopkins
            University Applied Physics Laboratory, 11100 Johns Hopkins Rd,
            Laurel, MD 20723, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(138..860)
                     /gene="rhlR"
                     /label="HTH-type quorum-sensing regulator RhlR"
                     /note="HTH-type quorum-sensing regulator RhlR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P54292"
     RBS             867..878
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      complement(870..878)
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(884..939)
                     /regulatory_class="promoter"
     regulatory      complement(893..898)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(916..921)
                     /regulatory_class="minus_35_signal"
     misc_feature    1018..1037
                     /label="qsc119 rhl box"
                     /note="qsc119 rhl box"
     RBS             1092..1103
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      1092..1100
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     CDS             1110..1823
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      1932..2018
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2188..2733)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2847..2941)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2975..3766)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaaccttac cagagggcgc cccagctggc aattccgacg tcattgcgtt gcgctcactg
       61 cgtaggctga aaatcttctc tcatccgcca aaacagccaa gcttgatggc gcgccggccg
      121 gcgcgccctg cgcttcagat gaggcccagc gccgcggcgt aggcggcagc cagcgtcttg
      181 ttcggcgcgt cgaacttctt ctggatgttc ttgtggtgga agttcaccgt gctctcggaa
      241 atgctcagga tgatggcgat ttccccggag ctcttgccgt cggcggtcca ttgcaggatc
      301 tcgcgttcgc gatggctcag gcagaccggg ttggacatca gcatcggatg ctccaggtcg
      361 gtcagcttct gggtcagcaa ctcgatcatg caacgcagcc gcaggcgtat ttcctcgcgc
      421 tcgaagctgg agatgttctg ctggtcgcgc gccacggaaa gcacgctgag caaattgttc
      481 ggcgcgcgga tcggcaaagt cgcgccgaca cagaggcccc aatcgcgagc ctcgttccag
      541 agcatccggc tctggtcgaa caggctgtcg ctccagacca ccatttccga ggagcgcagg
      601 ccgttgagga tcgccggatc cacggccccg tagttctgca tctggtatcg ctccagccag
      661 gccttgggat aggtgccatg gacctcggtc ttcggccggg tgaagggaat cgtatggcgc
      721 acgccatagg cgtagtaatc gaagcccagg cgccgcactt ccttttccag gacggcgaac
      781 acgccctggc tgtcgtggat cggctgcatc tcgctacgca aaccgtccca ccacagcaaa
      841 aagcctccgt cattcctcat gcttaatttc tcctctttgc tttccgggcg ctatcatgcc
      901 ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt aatctagagc taccgatcct
      961 gtgaaatctg gcagttattc cagcgaaaac atacagattt ccggcgaaat caaggcttcc
     1021 tgtgaaatct ggcagttacc gttagctttc gaattggcta aaaagtgttc atcggctacg
     1081 tacctagaat taaagaggag aaattaagca tgcgtaaagg agaagaactt ttcactggag
     1141 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1201 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1261 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
     1321 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     1381 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     1441 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1501 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     1561 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     1621 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     1681 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     1741 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     1801 atggcatgga tgaactatac aaaaggcctg cagcaaacga cgaaaactac gctttagtag
     1861 cttaataagc tggacatcac tctcggcatg gacgagctgt acaagtaatc ggccgtctcg
     1921 agtagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1981 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acctagggga
     2041 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     2101 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
     2161 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
     2221 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
     2281 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     2341 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     2401 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     2461 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     2521 cagccactgg taattggttt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     2581 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     2641 ctggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     2701 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
     2761 tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
     2821 cgatataagt tgttactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     2881 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     2941 ccaggcgagc tctcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     3001 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     3061 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     3121 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     3181 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg
     3241 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     3301 cctgatcgac aagaccggcc tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     3361 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     3421 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     3481 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     3541 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagc tcattcaggg
     3601 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     3661 cggcggcatc agagcagccg attgcctgtt gtgcccagtc atagccgaat agcctctcca
     3721 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc
     3781 atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag
     3841 ccatccagtt tactttgcag ggcttc
//
