LOCUS       pFN6K_(HQ).        3466 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Flexi(R) vector with a kanamycin resistance marker, for bacterial or
            in vitro expression of a protein with an N-terminal HQ metal 
            affinity tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFN6K (HQ)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Subclone a coding sequence between the SgfI/AsiSI and PmeI sites to 
            remove the lethal barnase gene.
FEATURES             Location/Qualifiers
     source          1..3466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             70..72
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             76..93
                     /label=HQ tag
                     /note="HQHQHQ metal affinity tag"
     CDS             125..457
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     misc_feature    470..525
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      589..636
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1017..1808
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(1983..2571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2687..2970
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     terminator      3200..3286
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3378..3405
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ggatctcgat cccgcgaaat taatacgact cactataggc aattttaact ttactaagga
       61 gaattcacca tgaaacatca acatcagcac caagcgatcg ccatggaata agtaaggaat
      121 ccacatggca caggttatca acacgtttga cggggttgcg gattatcttc agacatatca
      181 taagctacct gataattaca ttacaaaatc agaagcacaa gccctcggct gggtggcatc
      241 aaaagggaac cttgcagacg tcgctccggg gaaaagcatc ggcggagaca tcttctcaaa
      301 cagggaaggc aaactcccgg gcaaaagcgg acgaacatgg cgtgaagcgg atattaacta
      361 tacatcaggc ttcagaaatt cagaccggat tctttactca agcgactggc tgatttacaa
      421 aacaacggac cattatcaga cctttacaaa aatcagataa tgtttaaacg aattcgggct
      481 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagctgatcc ggctgctaac
      541 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      601 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccggt
      661 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
      721 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
      781 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag
      841 cccttgcgcc ctgagtgctt gcggcagcgt gagcttcaaa agaattgcca gctggggcgc
      901 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
      961 tctgatggcg caggggatca agatctgatc aagagacagg atgacggtcg tttcgcatgc
     1021 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     1081 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     1141 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     1201 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     1261 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     1321 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     1381 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     1441 agcgagcacg cactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     1501 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgtatg ccggatggtg
     1561 aggatctcgt cgtgactcat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     1621 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     1681 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     1741 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     1801 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaaccggta
     1861 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1921 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1981 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2161 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2281 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2341 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2401 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     2461 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2521 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatttca agaagatcct
     2581 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2641 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tatagtccgg aaatacagga
     2701 acgcacgctg gatggccctt cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg
     2761 gattaagtgg gggtaatgtg gcctgtaccc tctggttgca taggtattca tacggttaaa
     2821 atttatcagg cgcgattgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg
     2881 ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca attaagggat tatggtaaat
     2941 ccacttactg tctgccctcg tagccatcga gataaaccgc agtactccgg ccacgatgcg
     3001 tccggcgtag aggatcgaga tcttttcagc ctgatacaga ttaaatcaga acgcagaagc
     3061 ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     3121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
     3181 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3241 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     3301 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     3361 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     3421 tcttttgttt atttttctaa atacattcaa atatgtatcc gctcat
//
