LOCUS       40924_20396        6308 bp DNA     circular SYN 18-DEC-2018
DEFINITION  NanoLuc CMV-Hygro luciferase vector pFN31A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   McNamara B, Kopish K, Robers M.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2013) Scientific Communications, Promega 
            Corporation, 5500 E. Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 6308)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-OCT-2013) Scientific Communications, Promega Corporation, 5500 
            E. Cheryl Parkway, Madison, WI 53711, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1065..1577
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     misc_feature    1578..1589
                     /gene="NLuc"
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /gene="NLuc"
                     /note="used in Flexi vector cloning; SgfI site"
     CDS             1621..1953
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     misc_feature    1958..1965
                     /label=PmeI site
                     /note="PmeI site"
     misc_feature    1966..2021
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(2126..2247)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2492..2849
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2880..3914
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3941..3989
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        4145..4249
                     /label=AmpR promoter
     CDS             4250..5107
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(5268..5856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5972..6255
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatggtc ttcacactcg
     1081 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
     1141 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
     1201 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
     1261 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
     1321 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
     1381 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
     1441 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
     1501 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
     1561 gcgaacgcat tctggcgggc tcgagcggcg cgatcgccat ggaataagta aggaatccac
     1621 atggcacagg ttatcaacac gtttgacggg gttgcggatt atcttcagac atatcataag
     1681 ctacctgata attacattac aaaatcagaa gcacaagccc tcggctgggt ggcatcaaaa
     1741 gggaaccttg cagacgtcgc tccggggaaa agcatcggcg gagacatctt ctcaaacagg
     1801 gaaggcaaac tcccgggcaa aagcggacga acatggcgtg aagcggatat taactataca
     1861 tcaggcttca gaaattcaga ccggattctt tactcaagcg actggctgat ttacaaaaca
     1921 acggaccatt atcagacctt tacaaaaatc agataatgtt taaacgaatt cgggctcggt
     1981 acccggggat cctctagagt cgacctgcag gcatgcaagc tgatccggct gctaacaaag
     2041 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     2101 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     2161 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2221 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     2281 gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa atgtggtaaa
     2341 atcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     2401 catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa
     2461 cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg
     2521 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2581 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2641 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     2701 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2761 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     2821 gcttttttgg aggcctaggc ttttgcaaaa agctcgattc ttctgacact agcgccacca
     2881 tgaagaagcc cgaactcacc gctaccagcg ttgaaaaatt tctcatcgag aagttcgaca
     2941 gtgtgagcga cctgatgcag ttgtcggagg gcgaagagag ccgagccttc agcttcgatg
     3001 tcggcggacg cggctatgta ctgcgggtga atagctgcgc tgatggcttc tacaaagacc
     3061 gctacgtgta ccgccacttc gccagcgctg cactacccat ccccgaagtg ttggacatcg
     3121 gcgagttcag cgagagcctg acatactgca tcagtagacg cgcccaaggc gttactctcc
     3181 aagacctccc cgaaacagag ctgcctgctg tgttacagcc tgtcgccgaa gctatggatg
     3241 ctattgccgc cgccgacctc agtcaaacca gcggcttcgg cccattcggg ccccaaggca
     3301 tcggccagta cacaacctgg cgggatttca tttgcgccat tgctgatccc catgtctacc
     3361 actggcagac cgtgatggac gacaccgtgt ccgccagcgt agctcaagcc ctggacgaac
     3421 tgatgctgtg ggccgaagac tgtcccgagg tgcgccacct cgtccatgcc gacttcggca
     3481 gcaacaacgt cctgaccgac aacggccgca tcaccgccgt aatcgactgg tccgaagcta
     3541 tgttcgggga cagtcagtac gaggtggcca acatcttctt ctggcggccc tggctggctt
     3601 gcatggagca gcagactcgc tacttcgagc gccggcatcc cgagctggcc ggcagccctc
     3661 gtctgcgagc ctacatgctg cgcatcggcc tggatcagct ctaccagagc ctcgtggacg
     3721 gcaacttcga cgatgctgcc tgggctcaag gccgctgcga tgccatcgtc cgcagcgggg
     3781 ccggcaccgt cggtcgcaca caaatcgctc gccggagcgc agccgtatgg accgacggct
     3841 gcgtcgaggt gctggccgac agcggcaacc gccggcccag tacacgaccg cgcgctaagg
     3901 aggtaggtcg agtttagact ctagccatca cgatggccgc aataaaatat ctttattttc
     3961 attacatctg tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc tctttgcgct
     4021 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     4081 ctgcggactg gctttctacg taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4141 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4201 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4261 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4321 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4381 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4441 tccaatgatg agcactttca aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4501 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4561 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4621 cataaccatg agtgataaca ctgcggccaa cttacttctg acaactatcg gaggaccgaa
     4681 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4741 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4801 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4861 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4921 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4981 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5041 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5101 gcattggtaa ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc tcactcaaag
     5161 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5221 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5281 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5341 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5401 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5461 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5521 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5581 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5641 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5701 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5761 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5821 aagcagcaga ttacgcgcag aaaaaaagga tttcaagaag atcctttgat cttttctacg
     5881 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5941 aaaaggatct tcacctagat ccttttatag tccggaaata caggaacgca cgctggatgg
     6001 cccttcgctg ggatggtgaa accatgaaaa atggcagctt cagtggatta agtgggggta
     6061 atgtggcctg taccctctgg ttgcataggt attcatacgg ttaaaattta tcaggcgcga
     6121 ttgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc
     6181 tgaacgcgtt ttagcggtgc gtacaattaa gggattatgg taaatccact tactgtctgc
     6241 cctcgtagcc atcgagataa accgcagtac tccggccacg atgcgtccgg cgtagaggat
     6301 cgagatct
//
