LOCUS       62056_10930        6515 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1083..1523
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVA"
     CDS             1585..1917
                     /codon_start=1
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
                     /translation="MAQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLAD
                     VAPGKSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHY
                     QTFTKIR"
     misc_feature    1930..1985
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(2090..2211)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2456..2813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3155..4087
                     /codon_start=1
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    4148..4196
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        4352..4456
                     /label=AmpR promoter
     CDS             4457..5314
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(5475..6063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6179..6462
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagccag cttgaagcaa gcctcctgaa
     1081 agatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca
     1141 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct
     1201 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa
     1261 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga
     1321 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag
     1381 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc
     1441 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag
     1501 gtcaaagaca gttgactgta gcgattccgt cgacaccacc tactccctct ccagcgatcg
     1561 ccatggaata agtaaggaat ccacatggca caggttatca acacgtttga cggggttgcg
     1621 gattatcttc agacatatca taagctacct gataattaca ttacaaaatc agaagcacaa
     1681 gccctcggct gggtggcatc aaaagggaac cttgcagacg tcgctccggg gaaaagcatc
     1741 ggcggagaca tcttctcaaa cagggaaggc aaactcccgg gcaaaagcgg acgaacatgg
     1801 cgtgaagcgg atattaacta tacatcaggc ttcagaaatt cagaccggat tctttactca
     1861 agcgactggc tgatttacaa aacaacggac cattatcaga cctttacaaa aatcagataa
     1921 tgtttaaacg aattcgggct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
     1981 aagctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     2041 agcaataact agcataaccc cttggggcgg ccgcttcgag cagacatgat aagatacatt
     2101 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2161 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     2221 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag
     2281 taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa atattaacgc ttacaatttc
     2341 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgcggatct
     2401 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
     2461 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     2521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     2581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     2701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     2761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     2821 attcttctga cacaacagtc tcgaacttaa ggctagaatt ctggctacag gtaagcgccc
     2881 ctaaaatccc tttgggcaca atgtgtcctg aggggagagg cagcgacctg tagatgggac
     2941 gggggcacta accctcaggt ttggggcttc tgaatgtgag tatcgccatg taagcccagt
     3001 atttggccaa tctcagaaag ctcctggtcc ctggagggat ggagagagaa aaacaaacag
     3061 ctcctggagc agggagagtg ctggcctctt gctctccggc tccctctgtt gccctctggt
     3121 ttctccccag gctcccggac gtcctctagc caccatgact tcgaaagttt atgatccaga
     3181 acaaaggaaa cggatgataa ctggtccgca gtggtgggcc agatgtaaac aaatgaatgt
     3241 tcttgattca tttattaatt attatgattc agaaaaacat gcagaaaatg ctgttatttt
     3301 tttacatggt aacgcggcct cttcttattt atggcgacat gttgtgccac atattgagcc
     3361 agtagcgcgg tgtattatac cagaccttat tggtatgggc aaatcaggca aatctggtaa
     3421 tggttcttat aggttacttg atcattacaa atatcttact gcatggtttg aacttcttaa
     3481 tttaccaaag aagatcattt ttgtcggcca tgattggggt gcttgtttgg catttcatta
     3541 tagctatgag catcaagata agatcaaagc aatagttcac gctgaaagtg tagtagatgt
     3601 gattgaatca tgggatgaat ggcctgatat tgaagaagat attgcgttga tcaaatctga
     3661 agaaggagaa aaaatggttt tggagaataa cttcttcgtg gaaaccatgt tgccatcaaa
     3721 aatcatgaga aagttagaac cagaagaatt tgcagcatat cttgaaccat tcaaagagaa
     3781 aggtgaagtt cgtcgtccaa cattatcatg gcctcgtgaa atcccgttag taaaaggtgg
     3841 taaacctgac gttgtacaaa ttgttaggaa ttataatgct tatctacgtg caagtgatga
     3901 tttaccaaaa atgtttattg aatcggaccc aggattcttt tccaatgcta ttgttgaagg
     3961 tgccaagaag tttcctaata ctgaatttgt caaagtaaaa ggtcttcatt tttcgcaaga
     4021 agatgcacct gatgaaatgg gaaaatatat caaatcgttc gttgagcgag ttctcaaaaa
     4081 tgaacaataa ttctagccct gaataagtga taataagcgg atgaatggca gaaattcgtc
     4141 gaagcgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     4201 gatagcgata aggatcctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     4261 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtaa tggtttctta
     4321 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4381 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4441 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4501 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4561 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4621 tgagagtttt cgccccgaag aacgttttcc aatgatgagc actttcaaag ttctgctatg
     4681 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4741 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4801 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4861 acttctgaca actatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4921 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4981 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5041 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5101 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5161 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5221 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5281 cgctgagata ggtgcctcac tgattaagca ttggtaattc gaaatgaccg accaagcgac
     5341 gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5401 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5461 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5521 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5581 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5641 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5701 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5761 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5821 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5881 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     5941 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6001 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt
     6061 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6121 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttatagtcc
     6181 ggaaatacag gaacgcacgc tggatggccc ttcgctggga tggtgaaacc atgaaaaatg
     6241 gcagcttcag tggattaagt gggggtaatg tggcctgtac cctctggttg cataggtatt
     6301 catacggtta aaatttatca ggcgcgattg cggcagtttt tcgggtggtt tgttgccatt
     6361 tttacctgtc tgctgccgtg atcgcgctga acgcgtttta gcggtgcgta caattaaggg
     6421 attatggtaa atccacttac tgtctgccct cgtagccatc gagataaacc gcagtactcc
     6481 ggccacgatg cgtccggcgt agaggatcga gatct
//
