LOCUS       40924_20276        3669 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFM46, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Anderson JC, Voigt CA, Arkin AP.
  TITLE     Environmental signal integration by a modular AND gate
  JOURNAL   Mol. Syst. Biol. 3, 133 (2007)
  PUBMED    17700541
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Salis HM, Mirsky EA, Voigt CA.
  TITLE     Automated design of synthetic ribosome binding sites to control 
            protein expression
  JOURNAL   Nat. Biotechnol. 27 (10), 946-950 (2009)
  PUBMED    19801975
REFERENCE   3  (bases 1 to 3669)
  AUTHORS   Tamsir A, Tabor JJ, Voigt CA.
  TITLE     Robust multicellular computing using genetically encoded NOR gates 
            and chemical 'wires'
  JOURNAL   Nature 469 (7329), 212-215 (2011)
  PUBMED    21150903
REFERENCE   4  (bases 1 to 3669)
  AUTHORS   Moser F, Voigt CA.
  TITLE     Genetic Circuit Performance under Conditions Relevant for Industrial
            Bioreactors
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3669)
  AUTHORS   Anderson JC, Moser F, Tamsir A, Salis H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   6  (bases 1 to 3669)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 3669)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Syst. 
            Biol. 3, 133 (2007)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2009"; volume: "27"; issue: "10"; pages: 
            "946-950"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Nature"; 
            date: "2011"; volume: "469"; issue: "7329"; pages: "212-215"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (06-JAN-2012) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(413..957)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1447..1490)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1493..1514
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      1515..1549
                     /label=BBa_J23102
                     /note="BBa_J23102"
                     /regulatory_class="promoter"
     regulatory      1558..1570
                     /label=BBa_B0032
                     /note="BBa_B0032"
                     /regulatory_class="ribosome_binding_site"
     CDS             1577..2290
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      2313..2384
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2400..2427
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    2434..2454
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2455..2514
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(2675..3487)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
       61 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
      121 caccttcttc acgaggcaga cctcagcgct agcggagtgt atactggctt actatgttgg
      181 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
      241 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
      301 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
      361 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
      421 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
      481 cgacaggact ataaagatac caggcgtttc ccctggcggc tccctcgtgc gctctcctgt
      541 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
      601 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
      661 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
      721 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
      781 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
      841 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
      901 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
      961 gatcatctta ttaaggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1021 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1081 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1141 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1201 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1261 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1321 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattcc atggtgccac
     1381 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     1441 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc
     1501 ggccgcttct agagttgaca gctagctcag tcctaggtac tgtgctagct actagagtca
     1561 cacaggaaag tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
     1621 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     1681 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     1741 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
     1801 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     1861 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     1921 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1981 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     2041 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     2101 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     2161 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     2221 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     2281 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2341 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2401 gctcaccttc gggtgggcct ttctgcgttt atatactagt agcggccgct gcagtccggc
     2461 aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt
     2521 cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2581 gcggtatcag ctcactcaaa ggcggtaatc tcgagtcccg tcaagtcagc gtaatgctct
     2641 gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     2701 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     2761 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     2821 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     2881 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
     2941 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     3001 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     3061 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     3121 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     3181 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     3241 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     3301 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     3361 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     3421 aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat
     3481 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     3541 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt
     3601 gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag
     3661 accgttccg
//
