LOCUS       40924_20271        3714 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFM45, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
  PUBMED    24032656
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3714)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3714)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(413..957)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1447..1490)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1493..1514
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     misc_feature    1515..1594
                     /label=pAda
                     /note="pAda"
     misc_feature    1532..1549
                     /label=Ada operator
                     /note="Ada operator"
     misc_feature    1603..1615
                     /label=RBS B0032
                     /note="RBS B0032"
     CDS             1622..2335
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2358..2429
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2445..2472
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    2479..2499
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2500..2559
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(2720..3532)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc
       61 gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa
      121 caccttcttc acgaggcaga cctcagcgct agcggagtgt atactggctt actatgttgg
      181 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
      241 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
      301 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
      361 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
      421 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
      481 cgacaggact ataaagatac caggcgtttc ccctggcggc tccctcgtgc gctctcctgt
      541 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
      601 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
      661 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
      721 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
      781 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
      841 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
      901 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
      961 gatcatctta ttaaggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1021 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1081 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1141 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1201 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1261 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1321 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattcc atggtgccac
     1381 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     1441 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc
     1501 ggccgcttct agagaatctt ccttgtcagc gaaaaaaatt aaagcgcaag attgttggtt
     1561 tttgcgtgat ggtgaccggg cagcctaaag gctatactag agtcacacag gaaagtacta
     1621 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     1681 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     1741 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     1801 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     1861 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     1921 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     1981 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     2041 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     2101 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     2161 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     2221 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     2281 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     2341 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2401 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2461 ggcctttctg cgtttatata ctagtagcgg ccgctgcagt ccggcaaaaa aacgggcaag
     2521 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct
     2581 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2641 tcaaaggcgg taatctcgag tcccgtcaag tcagcgtaat gctctgccag tgttacaacc
     2701 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     2761 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     2821 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     2881 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     2941 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
     3001 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     3061 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     3121 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     3181 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     3241 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     3301 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     3361 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     3421 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     3481 tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc
     3541 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     3601 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt
     3661 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccg
//
