LOCUS       40924_20231       10925 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Flp expression vector pFLP3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP.
  TITLE     A Tn7-based broad-range bacterial cloning and expression system
  JOURNAL   Nat. Methods 2 (6), 443-448 (2005)
  PUBMED    15908923
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   Choi KH, Gaynor J, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Improved FRT cassettes and Flp expression vectors
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10925)
  AUTHORS   Choi KH, Gaynor J, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2004) Microbiology, Immunology and Pathology, 
            Colorado State University, Fort Collins, CO 80523, USA
REFERENCE   4  (bases 1 to 10925)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 10925)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2005"; volume: "2"; issue: "6"; pages: "443-448"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2004) Microbiology, Immunology and Pathology, Colorado State
            University, Fort Collins, CO 80523, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(232..583)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             597..1427
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     primer_bind     1591..1607
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2341..3759)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(3760..4205)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        4420..4448
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             4496..5683
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             complement(6057..7325)
                     /codon_start=1
                     /label=FLP
                     /note="site-specific recombinase"
                     /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     RBS             7329..7337
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7426..8136
                     /codon_start=1
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     primer_bind     complement(8428..8444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8452..8468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8476..8506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8521..8542)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            8926..9034
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(9106..9694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9868..10725)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10726..10830)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatacctc tcaggcgccg ctggtgccgc tggttggacg ccaagggtga atccgcctcg
      241 ataccctgat tactcgcttc ctgcgccctc tcaggcggcg ataggggact ggtaaaacgg
      301 ggattgccca gacgcctccc ccgccccttc aggggcacaa atgcggcccc aacggggcca
      361 cgtagtggtg cgttttttgc gtttccaccc ttttcttcct tttccctttt aaacctttta
      421 ggacgtctac aggccacgta atccgtggcc tgtagagttt aaaaagggac ggatttgttg
      481 ccattaaggg acggatttgt tgttaagaag ggacggattt gttgttgtaa agggacggat
      541 ttgttgtatt gtgggacgca gatacagtgt ccccttatac acaaggaatg tcgaacgtgg
      601 cctcaccccc aatggtttac aaaagcaatg ccctggtcga ggccgcgtat cgcctcagtg
      661 ttcaggaaca gcggatcgtt ctggcctgta ttagccaggt gaagaggagc gagcctgtca
      721 ccgatgaagt gatgtattca gtgacggcgg aggacatagc gacgatggcg ggtgtcccta
      781 tcgaatcttc ctacaaccag ctcaaagaag cggccctgcg cctgaaacgg cgggaagtcc
      841 ggttaaccca agagcccaat ggcaagggga aaagaccgag tgtgatgatt accggctggg
      901 tgcaaacaat catctaccgg gagggtgagg gccgtgtaga actcaggttc accaaagaca
      961 tgctgccgta cctgacggaa ctcaccaaac agttcaccaa atacgccttg gctgacgtgg
     1021 ccaagatgga cagcacccac gcgatcaggc tttacgagct gctcatgcaa tgggacagca
     1081 tcggccagcg cgaaatagaa attgaccagc tgcgaaagtg gtttcaactg gaaggccggt
     1141 atccctcgat caaggacttc aagttgcgag tgcttgatcc agccgtgacg cagatcaacg
     1201 agcacagccc gctacaggtg gagtgggcgc agcgaaagac cgggcgcaag gtcacacatc
     1261 tgttgttcag ttttggaccg aagaagcccg ccaaggcggt gggtaaggcc ccagcgaagc
     1321 gcaaggccgg gaagatttca gatgctgaga tcgcgaaaca ggctcgccct ggtgagacat
     1381 gggaagcggc ccgcgctcga ctaacccaga tgccgctgga tctggcctag aggccgtggc
     1441 caccacggcc cggcctgcct ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1501 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1561 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
     1621 gcctgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg
     1681 ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga
     1741 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag
     1801 tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg
     1861 ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt
     1921 cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt
     1981 ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca
     2041 cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca
     2101 cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa
     2161 agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca ttaccaaaag
     2221 gttaggaata cggttagcca tttgcctgct tttatatagt tcatatggga ttcaccttta
     2281 tgttgataag aaataaaaga aaatgccaat aggatatcgg cattttcttt tgcgttttta
     2341 tttgttaact gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt
     2401 gcctttgatg ttcagcagga agctaggcgc aaacgttgat tgtttgtctg cgtagaatcc
     2461 tctgtttgtc atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa
     2521 gtgtgagtaa gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt
     2581 tttgttcagc ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat
     2641 atcgttagac gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacagata
     2701 ccatttgccg ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga
     2761 tgcaatcagc ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc
     2821 gagagcgccg tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact
     2881 ttcttgacgg aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc
     2941 gccttggtag ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt
     3001 atcttctacg tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc
     3061 atcgatgaac tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata
     3121 atcctctaca ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt
     3181 tgtcagtgtt tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt
     3241 tccgtcagat gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga
     3301 atcatttgca tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc
     3361 aatagaagtt tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt
     3421 aggatctccg gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc
     3481 agcgttttgt aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt
     3541 aattgtggac gaatcgaact caggaacttg atatttttca tttttttgct gttcagggat
     3601 ttgcagcata tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg
     3661 gttcgtttct ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt
     3721 taatactgtt gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat
     3781 gtactgtgtt agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac
     3841 gcacaataaa aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct
     3901 ttacacattt taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga
     3961 cctcattcta ttagactctc gtttggattg caactggtct attttcctct tttgtttgat
     4021 agaaaatcat aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt
     4081 cttttcattc tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa
     4141 tcacaattca gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat
     4201 atgtgatggg ttaaaaagga tcgatcctct agagaatagg aacttcggaa taggaacttc
     4261 aagatcccct gattcccttt gtcaacagca atggatactg actcgctgcg ctcggtcgtt
     4321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4381 ggggataacg caggaaagaa catgaattaa ttctcatgtt tgacagctta tcatcgatta
     4441 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgcgtatgaa
     4501 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     4561 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     4621 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     4681 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     4741 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggattc tctacgccgg
     4801 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     4861 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     4921 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tacatgcacc
     4981 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     5041 ggagtcgcat aagggagagc gccgaccgat gcccttgaga gccttcaacc cagtcagctc
     5101 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     5161 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     5221 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     5281 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     5341 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     5401 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     5461 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     5521 tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc
     5581 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     5641 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg
     5701 cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact
     5761 gtgaatgcgc aaaccaaccc ttggggctgc aggaattcca ttgagtaagt ttttaagcac
     5821 atcagcttca aaagcgctct gaagttccta tactttctag aggatccccc tagagaatag
     5881 gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg
     5941 cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat
     6001 atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg
     6061 cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat
     6121 gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc
     6181 tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatatgca
     6241 tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg atgagtatac
     6301 gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac attagtcaac
     6361 tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat gtgagatttt
     6421 gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc tttattgtac
     6481 gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga agaattgccg
     6541 gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc caaatataca
     6601 agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac gcttgtcttt
     6661 gtctctgtca ctaaacactg gattattact cccagatact tattttggac taatttaaat
     6721 gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa agtagctagg
     6781 aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc aaacgaattt
     6841 agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc tttaagcatt
     6901 tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg taattgcaaa
     6961 ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg aataattgta
     7021 aattcccaag caggaatcaa tttctttaat gaggcttcca gaattgttgc tttttgcgtc
     7081 ttgtatttaa actggagtga tttattgaca atatcgaaac tcagcgaatt gcttatgata
     7141 gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt aatcatccaa
     7201 cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg tctttcaaac
     7261 ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat accaaattgt
     7321 ggcatacaac ctccttagta catgcaacca ttatcaccgc cagaggtaaa atagtcaaca
     7381 cgcacggtgt tagatattta tcccttgcgg tgatagattt aacgtatgag cacaaaaaag
     7441 aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa
     7501 aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag
     7561 tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg
     7621 cttacaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc
     7681 tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct
     7741 gttttttctc atgttcaggc agggatgttc tcacctaagc ttagaacctt taccaaaggt
     7801 gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag
     7861 gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg
     7921 ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt
     7981 gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa
     8041 ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa
     8101 gttatcgcta gtcagtggcc tgaagagacg tttggctgat cggcaaggtg ttctggtcgg
     8161 cgcatagctg ataacaattg agcaagaatc ttcatcgaat taggggaatt ttcactcccc
     8221 tcagaacata acatagtaaa tggattgaat tatgaagaat ggtttttatg cgacttaccg
     8281 cagcaaaaat aaagggaaag ataagcgctc aataaacctg tctgttttcc ttaattctct
     8341 gctggctgat aatcatcacc tgcaggttgg ctccaattat ttgtatattc ataaaatcgg
     8401 ggtaccgagc tcgaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8761 aggaaagaac atggggattc cttaaggtat actttccgct gcataaccct gcttcggggt
     8821 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca
     8881 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga
     8941 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt
     9001 gctcaacggg aatcctgctc tgcgaggctg gccgataagc tagcttatgt gagcaaaagg
     9061 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9301 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9541 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9781 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9841 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9901 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9961 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10021 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10081 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10141 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10201 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10261 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10321 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10381 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10441 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10501 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10561 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10621 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10681 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10741 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10801 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10861 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10921 tcgtc
//
