LOCUS       40924_20186        3742 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Hernandez-Garcia CM, Bouchard RA, Rushton PJ, Jones ML, Chen X, 
            Timko MP, Finer JJ.
  TITLE     High level transgenic expression of soybean (Glycine max) GmERF and 
            Gmubi gene promoters isolated by a novel promoter analysis pipeline
  JOURNAL   BMC Plant Biol. 10, 237 (2010)
  PUBMED    21050446
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Finer JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2016) Horticulture and Crop Science, The Ohio 
            State University, 1680 Madison Ave., Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 3742)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol. 10, 237 (2010)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-APR-2016) Horticulture and Crop Science, The Ohio State 
            University, 1680 Madison Ave., Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             462..1178
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      1214..1466
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1521..1537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1545..1561)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1569..1599)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1614..1635)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1923..2511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2685..3542)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3543..3647)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgcag ctagcggtac
      421 cactagtccc gggtctagag tcgacgccgg ctcgaggatc catggtgagc aagggcgagg
      481 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      541 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      601 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcacct
      661 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      721 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      781 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      841 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      901 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      961 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1021 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1081 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1141 ccgccgggat cactcacggc atggacgagc tgtacaagta aagcggccgc ccggctgcac
     1201 tgaggcctca gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     1261 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     1321 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     1381 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     1441 cgcggtgtca tctatgttac tagatccgat gataagctgt caaacatgag aattagatct
     1501 caattgaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1561 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1621 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1681 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1741 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     2401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3601 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3661 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     3721 cgtatcacga ggccctttcg tc
//
