LOCUS       V016864                 6199 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016864
VERSION     V016864
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..157
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     enhancer        318..697
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             935..958
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             980..2491
                     /gene="YAP1"
                     /label="Transcriptional coactivator YAP1"
                     /note="Transcriptional coactivator YAP1 from Homo sapiens.
                     Accession#: P46937"
     polyA_signal    2540..3162
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     promoter        3191..3520
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(3559..3577)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3591..3607)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3615..3631)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3639..3669)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3684..3705)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3993..4581)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4755..5612)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5613..5717)
                     /label="AmpR promoter"
     rep_origin      5744..6199
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tgcggccgcg aattcatcga tagatcccgg gcagcagccg ccgcctcaac cggcccccca
     1021 gggccaaggg cagccgcctt cgcagccccc gcaggggcag ggcccgccgt ccggacccgg
     1081 gcaaccggca cccgcggcga cccaggcggc gccgcaggca ccccccgccg ggcatcagat
     1141 cgtgcacgtc cgcggggact cggagaccga cctggaggcg ctcttcaacg ccgtcatgaa
     1201 ccccaagacg gccaacgtgc cccagaccgt gcccatgagg ctccggaagc tgcccgactc
     1261 cttcttcaag ccgccggagc ccaaatccca ctcccgacag gccagtactg atgcaggcac
     1321 tgcaggagcc ctgactccac agcatgttcg agctcattcc tctccagctt ctctgcagtt
     1381 gggagctgtt tctcctggga cactgacccc cactggagta gtctctggcc cagcagctac
     1441 acccacagct cagcatcttc gacagtcttc ttttgagata cctgatgatg tacctctgcc
     1501 agcaggttgg gagatggcaa agacatcttc tggtcagaga tacttcttaa atcacatcga
     1561 tcagacaaca acatggcagg accccaggaa ggccatgctg tcccagatga acgtcacagc
     1621 ccccaccagt ccaccagtgc agcagaatat gatgaactcg gcttcaggtc ctcttcctga
     1681 tggatgggaa caagccatga ctcaggatgg agaaatttac tatataaacc ataagaacaa
     1741 gaccacctct tggctagacc caaggcttga ccctcgtttt gccatgaacc agagaatcag
     1801 tcagagtgct ccagtgaaac agccaccacc cctggctccc cagagcccac agggaggcgt
     1861 catgggtggc agcaactcca accagcagca acagatgcga ctgcagcaac tgcagatgga
     1921 gaaggagagg ctgcggctga aacagcaaga actgcttcgg caggcaatgc ggaatatcaa
     1981 tcccagcaca gcaaattctc caaaatgtca ggagttagcc ctgcgtagcc agttaccaac
     2041 actggagcag gatggtggga ctcaaaatcc agtgtcttct cccgggatgt ctcaggaatt
     2101 gagaacaatg acgaccaata gctcagatcc tttccttaac agtggcacct atcactctcg
     2161 agatgagagt acagacagtg gactaagcat gagcagctac agtgtccctc gaaccccaga
     2221 tgacttcctg aacagtgtgg atgagatgga tacaggtgat actatcaacc aaagcaccct
     2281 gccctcacag cagaaccgtt tcccagacta ccttgaagcc attcctggga caaatgtgga
     2341 ccttggaaca ctggaaggag atggaatgaa catagaagga gaggagctga tgccaagtct
     2401 gcaggaagct ttgagttctg acatccttaa tgacatggag tctgttttgg ctgccaccaa
     2461 gctagataaa gaaagctttc ttacatggtt atagagccct caggcagact gaattctgca
     2521 gtcgactcta gaggatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     2581 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     2641 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     2701 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     2761 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     2821 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     2881 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     2941 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     3001 tcccttccct gtccttctga ttttaaaata actataccag caggaggacg tccagacaca
     3061 gcataggcta cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt
     3121 cctctcatgc gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg
     3181 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3241 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3301 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     3361 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3421 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3481 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcctcg aggaactgaa
     3541 aaaccagaaa gttaattccc tatagtgagt cgtattaaat tcgtaatcat gtcatagctg
     3601 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3661 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3721 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3781 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3841 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3901 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3961 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4021 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4081 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4141 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4201 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4261 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4321 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4381 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4441 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4501 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4561 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4621 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4681 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4741 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4801 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4861 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4921 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4981 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5041 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5101 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5161 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5221 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5281 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5341 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5401 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5461 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5521 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5581 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5641 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5701 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     5761 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5821 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5881 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5941 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6001 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6061 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6121 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6181 atattaacgc ttacaattt
//
