LOCUS       pFLAG-Myc-CMV-19        4758 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression and secretion of FLAG- and Myc-tagged proteins
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pFLAG-Myc-CMV-19
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Sigma-Aldrich
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             932..976
                     /label=preprotrypsin leader
                     /note="leader sequence from mouse preprotrypsin"
     CDS             977..1000
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    998..1060
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1061..1090
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     polyA_signal    1099..1721
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1750..2079
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(2118..2136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2150..2166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2174..2190)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2198..2228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2243..2264)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2552..3140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3314..4171)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4172..4276)
                     /label=AmpR promoter
     rep_origin      4303..4758
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaattcac catgtctgca cttctgatcc tagctcttgt
      961 tggagctgca gttgctgact acaaagacga tgacgacaag cttgcggccg cgaattcatc
     1021 gatagatctg atatcggtac cagtcgactc tagaggatcc gaacaaaaac tcatctcaga
     1081 agaggatctg tgacccccgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     1141 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     1201 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     1261 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     1321 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     1381 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     1441 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     1501 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     1561 cccttccctg tccttctgat tttaaaataa ctataccagc aggaggacgt ccagacacag
     1621 cataggctac ctggccatgc ccaaccggtg ggacatttga gttgcttgct tggcactgtc
     1681 ctctcatgcg ttgggtccac tcagtagatg cctgttgaat tgggtacgcg gccagcttgg
     1741 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     1801 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     1861 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     1921 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     1981 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     2041 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcctcga ggaactgaaa
     2101 aaccagaaag ttaattccct atagtgagtc gtattaaatt cgtaatcatg tcatagctgt
     2161 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2221 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2281 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2341 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2401 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2461 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2521 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2581 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2641 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2701 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2761 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2821 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2881 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2941 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3001 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3061 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3121 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3181 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3241 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3601 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3901 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     4321 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4381 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4441 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4501 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4561 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4621 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4681 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4741 tattaacgct tacaattt
//
