LOCUS       pFLAG-CMV-1.        4732 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression and secretion of N-terminally FLAG-tagged 
            proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pFLAG-CMV-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Sigma-Aldrich
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..150
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        311..690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        691..894
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             925..969
                     /label=preprotrypsin leader
                     /note="leader sequence from mouse preprotrypsin"
     CDS             970..993
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    991..1057
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1055..1677
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1706..2035
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(2074..2092)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2106..2122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2130..2146)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2154..2184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2199..2220)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2508..3096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3270..4127)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4128..4232)
                     /label=AmpR promoter
     rep_origin      4259..4714
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
       61 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      121 cagtcacgac gttgtaaaac gacggccagt gccaagctga tctatacatt gaatcaatat
      181 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
      241 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
      301 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      361 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      421 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      481 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      541 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
      601 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
      661 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
      721 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      781 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
      841 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      901 gtgaaccgtc agaattaatt caccatgtct gcacttctga tcctagctct tgttggagct
      961 gcagttgctg actacaaaga cgatgacgac aagcttgcgg ccgcgaattc atcgatagat
     1021 ctgatatcgg taccagtcga ctctagagga tcccgggtgg catccctgtg acccctcccc
     1081 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa
     1141 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg
     1201 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg
     1261 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt
     1321 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag
     1381 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt
     1441 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca
     1501 ggcgtgaacc actgctccct tccctgtcct tctgatttta aaataactat accagcagga
     1561 ggacgtccag acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg
     1621 cttgcttggc actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg
     1681 tacgcggcca gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     1741 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     1801 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     1861 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     1921 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     1981 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     2041 cctcgaggaa ctgaaaaacc agaaagttaa ttccctatag tgagtcgtat taaattcgta
     2101 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2161 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2221 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2281 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2341 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2401 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2461 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2521 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2581 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2641 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2701 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2761 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2821 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2881 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2941 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3001 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3061 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3781 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4201 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4261 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4321 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4381 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4441 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4501 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4561 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4621 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4681 gcgaatttta acaaaatatt aacgcttaca atttccatac aatttccatt cg
//
