LOCUS       40924_20091        8652 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFL122, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Lauro FM, Eloe EA, Liverani N, Bertoloni G, Bartlett DH.
  TITLE     Conjugal vectors for cloning, expression, and insertional 
            mutagenesis in gram-negative bacteria
  JOURNAL   BioTechniques 38 (5), 708 (2005)
  PUBMED    15945369
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Lauro FM, Eloe EA, Liverani N, Bertoloni G, Bartlett DH.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2004) Marine Biology Research Division, Scripps 
            Institution of Oceanography, 8750 Biological Grade, La Jolla, CA 
            92037, USA
REFERENCE   3  (bases 1 to 8652)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8652)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2005"; volume: "38"; issue: "5"; pages: 
            "708"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2004) Marine Biology Research Division, Scripps Institution 
            of Oceanography, 8750 Biological Grade, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..866
                     /codon_start=1
                     /product="Sm resistance protein A"
                     /label=Sm resistance protein A
                     /protein_id="AAV49010.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     CDS             866..1702
                     /codon_start=1
                     /product="Sm resistance protein B"
                     /label=Sm resistance protein B
                     /protein_id="AAV49011.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     rep_origin      complement(2346..2740)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(2767..3051)
                     /codon_start=1
                     /product="mobilization protein C"
                     /label=mobilization protein C
                     /protein_id="AAV49012.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     oriT            3082..3169
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             3998..4411
                     /codon_start=1
                     /product="mobilization protein B"
                     /label=mobilization protein B
                     /protein_id="AAV49014.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     CDS             4408..5376
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5440..5652
                     /codon_start=1
                     /product="unknown protein E"
                     /label=unknown protein E
                     /protein_id="AAV49016.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             5654..5860
                     /codon_start=1
                     /product="repressor protein F"
                     /label=repressor protein F
                     /protein_id="AAV49017.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             5890..6726
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             6716..7564
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     8102..8118
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8128..8146
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8155..8262
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(8275..8293)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8314..8330)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8338..8354)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8362..8392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8407..8428)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc
       61 cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag
      121 gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg
      181 cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag
      241 gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct
      301 tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca
      361 aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat
      421 gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca
      481 atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg cacgatcttt
      541 tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg
      601 tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
      661 cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
      721 tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
      781 tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
      841 gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
      901 cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
      961 gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
     1021 actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
     1081 tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
     1141 tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
     1201 tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
     1261 tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
     1321 ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
     1381 tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
     1441 tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
     1501 cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
     1561 ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
     1621 ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
     1681 ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
     1741 atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
     1801 acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
     1861 tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
     1921 cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
     1981 gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
     2041 aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
     2101 gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
     2161 taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
     2221 gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
     2281 agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
     2341 tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
     2401 aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
     2461 gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
     2521 tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
     2581 ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
     2641 gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
     2701 cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
     2761 gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg
     2821 gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc actcgctgct
     2881 gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt cgttcttgcg
     2941 ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc gcgctcgttg
     3001 ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca tcttgacacc
     3061 ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc aatcccgacc
     3121 ctactttgta ggggagggcg cacttaccgg tttctcttcg agaaactggc ctaacggcca
     3181 cccttcgggc ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca aaagcaacag
     3241 cgaggcagca tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa
     3301 tcggccaggg ccaaggccga ctacatccag cgcgaaggca agtatgcccg cgacatggat
     3361 gaagtcttgc acgccgaatc cgggcacatg ccggagttcg tcgagcggcc cgccgactac
     3421 tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt
     3481 gccctgccgg tcgagctgac cctcgaccag cagaaggcgc tggcgtccga gttcgcccag
     3541 cacctgaccg gtgccgagcg cctgccgtat acgctggcca tccatgccgg tggcggcgag
     3601 aacccgcact gccacctgat gatctccgag cggatcaatg acggcatcga gcggcccgcc
     3661 gctcagtggt tcaagcggta caacggcaag accccggaga agggcggggc acagaagacc
     3721 gaagcgctca agcccaaggc atggcttgag cagacccgcg aggcatgggc cgaccatgcc
     3781 aaccgggcat tagagcgggc tggccacgac gcccgcattg accacagaac acttgaggcg
     3841 cagggcatcg agcgcctgcc cggtgttcac ctggggccga acgtggtgga gatggaaggc
     3901 cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc caacgcccag
     3961 atcatcgact tacaggaata ccgggaggca atagaccatg aacgcaatcg acagagtgaa
     4021 gaaatccaga ggcatcaacg agttagcgga gcagatcgaa ccgctggccc agagcatggc
     4081 gacactggcc gacgaagccc ggcaggtcat gagccagacc cagcaggcca gcgaggcgca
     4141 ggcggcggag tggctgaaag cccagcgcca gacaggggcg gcatgggtgg agctggccaa
     4201 agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc
     4261 gcgggggtgg cactggaagc tatggctaac cgtgatgctg gcttccatga tgcctacggt
     4321 ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg ccactgacaa ccgaggacgg
     4381 ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca ggactttgca ggccataggc
     4441 cgacagctca aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga cgccaccacc
     4501 ggccagatga tgaaccggga atggtcagcc gccgaagtgc tccagaacac gccatggctc
     4561 aagcggatga atgcccaggg caatgacgtg tatatcaggc ccgccgagca ggagcggcat
     4621 ggtctggtgc tggtggacga cctcagcgag tttgacctgg atgacatgaa agccgagggc
     4681 cgggagcctg ccctggtagt ggaaaccagc ccgaagaact atcaggcatg ggtcaaggtg
     4741 gccgacgccg caggcggtga acttcggggg cagattgccc ggacgctggc cagcgagtac
     4801 gacgccgacc cggccagcgc cgacagccgc cactatggcc gcttggcggg cttcaccaac
     4861 cgcaaggaca agcacaccac ccgcgccggt tatcagccgt gggtgctgct gcgtgaatcc
     4921 aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc aggctggcca gcagatcgag
     4981 caggcccagc ggcagcagga gaaggcccgc aggctggcca gcctcgaact gcccgagcgg
     5041 cagcttagcc gccaccggcg cacggcgctg gacgagtacc gcagcgagat ggccgggctg
     5101 gtcaagcgct tcggtgatga cctcagcaag tgcgacttta tcgccgcgca gaagctggcc
     5161 agccggggcc gcagtgccga ggaaatcggc aaggccatgg ccgaggccag cccagcgctg
     5221 gcagagcgca agcccggcca cgaagcggat tacatcgagc gcaccgtcag caaggtcatg
     5281 ggtctgccca gcgtccagct tgcgcgggcc gagctggcac gggcaccggc accccgccag
     5341 cgaggcatgg acaggggcgg gccagatttc agcatgtagt gcttgcgttg gtactcacgc
     5401 ctgttatact atgagtactc acgcacagaa gggggtttta tggaatacga aaaaagcgct
     5461 tcagggtcgg tctacctgat caaaagtgac aagggctatt ggttgcccgg tggctttggt
     5521 tatacgtcaa acaaggccga ggctggccgc ttttcagtcg ctgatatggc cagccttaac
     5581 cttgacggct gcaccttgtc cttgttccgc gaagacaagc ctttcggccc cggcaagttt
     5641 ctcggtgact gatatgaaag accaaaagga caagcagacc ggcgacctgc tggccagccc
     5701 tgacgctgta cgccaagcgc gatatgccga gcgcatgaag gccaaaggga tgcgtcagcg
     5761 caagttctgg ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg aagaactcag
     5821 agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg cctgcaaagg
     5881 aggcaatcaa tggctaccca taagcctatc aatattctgg aggcgttcgc agcagcgccg
     5941 ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc gctggtgtcg
     6001 cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat tgcaggcggg
     6061 ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct gcccgccgaa
     6121 gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct cagcgccgag
     6181 gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag cctgcccaac
     6241 atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg ccgcctgatg
     6301 gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg ccccatggcc
     6361 caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat cgtgttcctg
     6421 caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca ggccagccgg
     6481 ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag catgaccagc
     6541 gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt ccgcttcggt
     6601 gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg gcatgacggc
     6661 ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt gccccgtggt
     6721 gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc actgtctggc
     6781 ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc tggacgtgac
     6841 gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc tgggcgctga
     6901 tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc tagtgcttgg
     6961 cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac ccaagtggga
     7021 ggccgtcacc gctgaatgcc atgtggtcaa aggtagctat cgggcgctgg caaaggaaat
     7081 cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca tcgagcgcct
     7141 ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc ggctgctgtc
     7201 ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc ccttgatcgc
     7261 gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg tgcgggcgct
     7321 ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg accccggcaa
     7381 aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag aggccagtgg
     7441 ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg ttgccggagc tggtcgcgct
     7501 gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc ccaaggcggc
     7561 aggctgaccc cccccactct attgtaaaca agacattttt atcttttata ttcaatggct
     7621 tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat
     7681 attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt
     7741 tgcttccaga tgtatgctct ttgctcctgc atagatctat tcatccgctt attatcactt
     7801 attcaggcgt agcaaccagg cgtttaaggg caccaataac tgccttaaaa agattacgcc
     7861 ccgccctgcc actcatcgca gtacggccta ttggttaaaa aatgagctga tttaacaaaa
     7921 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
     7981 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8041 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8101 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggagctc
     8161 caccgcggtg gcggccgctc tagaactagt ggatcccccg ggctgcagga attcgatatc
     8221 aagcttatcg ataccgtcga cctcgagggg gggcccggta cccagctttt gttcccttta
     8281 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8341 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8401 tgcctaatga gtgagctaac tcacattaac tgcgttgcgc tcactgcccg ctttccagtc
     8461 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8521 gcgtattggg cgcatgcagc taatggatca ccgcaaacag gttactcgcc tggggattcc
     8581 ctttcgaccc gagcatccgt atgatactca tactcgatta ttattattat agaagcctga
     8641 tctcaattcg tt
//
