LOCUS       40924_20066        5261 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFHC2102, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Atlung T, Hansen FG.
  TITLE     Effect of different concentrations of H-NS protein on chromosome 
            replication and the cell cycle in Escherichia coli
  JOURNAL   J. Bacteriol. 184 (7), 1843-1850 (2002)
  PUBMED    11889089
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Hansen FG, Atlung T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2001) BioCentrum-DTU, Technical University of 
            Denmark, Building 301, Lyngby DK-2800, Denmark
REFERENCE   3  (bases 1 to 5261)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5261)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2002"; volume: "184"; issue: "7"; pages: 
            "1843-1850"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2001) BioCentrum-DTU, Technical University of Denmark, 
            Building 301, Lyngby DK-2800, Denmark"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1
                     /label=SP6 transcription initiation site
                     /note="SP6 transcription initiation site"
     misc_feature    9..75
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      complement(58..64)
                     /label=Shine-Dalgarno sequence
                     /note="Shine-Dalgarno sequence"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    89..107
                     /gene="lacI"
                     /label=lac operator (O)
                     /note="lac operator (O)"
     protein_bind    91..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(123..139)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        383..460
                     /label=lacI promoter
     CDS             461..1540
                     /label=lacI
                     /note="lac repressor"
     CDS             1891..2079
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2184..2326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     protein_bind    2417..2425
                     /label=DnaA binding site
                     /bound_moiety="DnaA"
     rep_origin      complement(2512..3100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3274..4131)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4132..4236)
                     /label=AmpR promoter
     rep_origin      4572..5027
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(5126..5173)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5245..5261
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatactcaa gcttcgaatt cgatatcgga tccgcggccg catgcatagt catatggcct
       61 ccttaatcta gagctcgagg ctgtgtgaaa ttgttatccg ctcacaattg aatctagtat
      121 cattgttatc cgctcacaag tcaacactct ttttgataaa ttttctcgaa tttgccagaa
      181 ccgttatgat gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg
      241 aattatgcag tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg
      301 ccagaagcat tggtgcaccg tgcagtcgat aagcccggat cagcttgcaa ttcgcgcgcg
      361 aaggcgaagc ggcatttacg ttgacaccat cgaatggcgc aaaacctttc gcggtatggc
      421 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
      481 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
      541 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
      601 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
      661 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
      721 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
      781 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
      841 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
      901 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
      961 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     1021 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     1081 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     1141 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     1201 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     1261 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     1321 caccagcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     1381 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     1441 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1501 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1561 taagttagcg cgaattgcaa gctggccgac gcgctgggct acgtcttgct ggcgttcgtc
     1621 gaggtgaaga cgaaagggcc cctcgactaa gttaacctaa ccagcccgcc taatgagcgg
     1681 gctttttttt ctcgacagat ctcccgctgg cattgaccct gagtgatttt tctctggtcc
     1741 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca
     1801 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg
     1861 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac
     1921 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac
     1981 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
     2041 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     2101 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     2161 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     2221 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg
     2281 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     2341 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2401 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2461 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2521 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2581 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2641 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2701 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2761 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2821 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2881 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2941 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3001 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3061 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3121 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3181 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3241 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3301 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3361 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3421 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3481 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3541 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     3601 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3661 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3721 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3781 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3841 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     3901 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3961 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4021 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4081 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4141 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4201 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4261 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     4321 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     4381 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     4441 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     4501 actgagagtg caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     4561 cgcatcaggc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4621 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4681 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4741 gttccgattt agagctttac ggcacctcga ccgcaaaaaa cttgatttgg gtgatggttc
     4801 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4861 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4921 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4981 acaaatattt aacgcgaatt ttaacaaaat attaacgttt acaatttcca ttcgccattc
     5041 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccaggtt
     5101 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
     5161 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
     5221 tgttagcagc cggatcaagc tgggatttag gtgacactat a
//
