LOCUS       40924_20061        4694 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFHC2101, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Atlung T, Hansen FG.
  TITLE     Effect of different concentrations of H-NS protein on chromosome 
            replication and the cell cycle in Escherichia coli
  JOURNAL   J. Bacteriol. 184 (7), 1843-1850 (2002)
  PUBMED    11889089
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Hansen FG, Atlung T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2001) BioCentrum-DTU, Technical University of 
            Denmark, Building 301, Lyngby DK-2800, Denmark
REFERENCE   3  (bases 1 to 4694)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2002"; volume: "184"; issue: "7"; pages: 
            "1843-1850"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2001) BioCentrum-DTU, Technical University of Denmark, 
            Building 301, Lyngby DK-2800, Denmark"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1
                     /label=SP6 transcription initiation site
                     /note="SP6 transcription initiation site"
     misc_feature    10..74
                     /note="multiple cloning site; synthetic adapters"
     regulatory      58..64
                     /gene="lacI"
                     /label=Shine-Dalgarno sequence
                     /note="Shine-Dalgarno sequence"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    89..107
                     /gene="lacI"
                     /label=lac operator (O)
                     /note="lac operator (O)"
     protein_bind    91..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(123..139)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        383..460
                     /label=lacI promoter
     CDS             461..1540
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1850..1858
                     /label=DnaA binding site
                     /bound_moiety="DnaA"
     rep_origin      complement(1945..2533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2707..3564)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3565..3669)
                     /label=AmpR promoter
     misc_feature    3949..3950
                     /note="inserted with Klenow polymerase to remove NdeI site"
     rep_origin      4005..4460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(4559..4606)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4678..4694
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaatactcaa gcttcgaatt cgatatcgga tccgcggccg catgcatagt catatggcct
       61 ccttaatcta gagctcgagg ctgtgtgaaa ttgttatccg ctcacaattg aatctagtat
      121 cattgttatc cgctcacaag tcaacactct ttttgataaa ttttctcgaa tttgccagaa
      181 ccgttatgat gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg
      241 aattatgcag tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg
      301 ccagaagcat tggtgcaccg tgcagtcgat aagcccggat cagcttgcaa ttcgcgcgcg
      361 aaggcgaagc ggcatttacg ttgacaccat cgaatggcgc aaaacctttc gcggtatggc
      421 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
      481 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
      541 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
      601 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
      661 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
      721 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
      781 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
      841 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
      901 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
      961 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     1021 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     1081 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     1141 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     1201 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     1261 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     1321 caccagcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     1381 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     1441 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1501 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1561 taagttagcg cgaattgcaa gctggccgac gcgctgggct acgtcttgct ggcgttcgtc
     1621 gaggtgaaga cgaaagggcc cctcgactaa gttaacctaa ccagcccgcc taatgagcgg
     1681 gctttttttt ctcgacagat ctcccgggct gcattaatga atcggccaac gcgcggggag
     1741 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1801 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1861 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1921 taaaaaggcc gcgttgctgg cgtttttcga taggctccgc ccccctgacg agcatcacaa
     1981 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2041 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2101 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2161 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2221 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2281 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2341 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2401 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2461 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2521 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2581 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2641 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2701 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2761 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2821 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2881 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2941 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3001 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3061 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3121 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3181 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3241 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3301 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3361 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3421 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3481 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3541 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3601 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3661 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     3721 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     3781 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     3841 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     3901 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt
     3961 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgacgcgc cctgtagcgg
     4021 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4081 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4141 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagagctt tacggcacct
     4201 cgaccgcaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4261 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4321 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4381 ttcggcctat tggttaaaaa atgagctgat ttaacaaata tttaacgcga attttaacaa
     4441 aatattaacg tttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     4501 tcggtgcggg cctcttcgct attacgccag gttatccgga tatagttcct cctttcagca
     4561 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg
     4621 tggcagcagc caactcagct tcctttcggg ctttgttagc agccggatca agctgggatt
     4681 taggtgacac tata
//
