LOCUS       40924_20046       11944 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pFH17, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   Hahn F, Mantegazza O, Greiner A, Hegemann P, Eisenhut M, Weber AP.
  TITLE     An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis 
            thaliana
  JOURNAL   Front Plant Sci 8, 39 (2017)
  PUBMED    28174584
REFERENCE   2  (bases 1 to 11944)
  AUTHORS   Hahn F, Mantegazza O, Eisenhut M, Greiner A, Hegemann P, Weber APM.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2016) Plant Biochemistry, Heinrich Heine 
            University Dusseldorf, Universitatsstr. 1, Dusseldorf 40225, Germany
REFERENCE   3  (bases 1 to 11944)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11944)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci 8, 39 (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2016) Plant Biochemistry, Heinrich Heine University 
            Dusseldorf, Universitatsstr. 1, Dusseldorf 40225, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(797..937)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1281..1475)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1544..2608)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(3045..3671)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    complement(4971..4995)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    5160..5181
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5196..5226
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5234..5250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5258..5274
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6034..6213
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     gene            6255..6807
                     /gene="bar1"
                     /label=bar1
                     /note="null allele"
     terminator      6827..7079
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      7104..7745
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(7758..7774)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8356..8608)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(8807..9598)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(9628..10008)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    complement(10380..10404)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10932..11720
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
      841 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
      901 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
      961 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     1021 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     1081 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     1141 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     1201 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     1261 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     1321 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     1381 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     1441 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     1501 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     1561 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     1621 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     1681 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     1741 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     1801 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     1861 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     1921 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     1981 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     2041 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     2101 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     2161 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     2221 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     2281 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     2341 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     2401 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
     2461 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
     2521 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
     2581 tttgtaccgg gccggatggt ttgcgaccgt cacgccgatt cctcgggctt gggggttcca
     2641 gtgccattgc agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct
     2701 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
     2761 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
     2821 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
     2881 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
     2941 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
     3001 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
     3061 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
     3121 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
     3181 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
     3241 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
     3301 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
     3361 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
     3421 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
     3481 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
     3541 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
     3601 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
     3661 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
     3721 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
     3781 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
     3841 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
     3901 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
     3961 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
     4021 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
     4081 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
     4141 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
     4201 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
     4261 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
     4321 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
     4381 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
     4441 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
     4501 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
     4561 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
     4621 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
     4681 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
     4741 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
     4801 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg
     4861 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
     4921 tcacgccctt ttaaatatcc gttattctaa taaacgctct tttctcttag gtttacccgc
     4981 caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc
     5041 aagctcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     5101 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     5161 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     5221 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     5281 tacgaattcg agctcggtac ccgacgagtc agtaataaac ggcgtcaaag tggttgcagc
     5341 cggcacacac gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa
     5401 taaggcaatt agccaaaaac aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt
     5461 attattagct attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc
     5521 ttcttcttct tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca
     5581 atttctcaaa atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc
     5641 tgtgttcctt attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata
     5701 tgttctttgg tttagattct gttaatctta gatcgaagac gattttctgg gtttgatcgt
     5761 tagatatcat cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa
     5821 tttgagtttt gtcgaataat tactcttcga tttgtgattt ctatctagat ctggtgttag
     5881 tttctagttt gtgcgatcga atttgtagat taatctgagt ttttctgatt aacagctcga
     5941 gtgcggtatc gatcatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac
     6001 gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactga
     6061 gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat tattgcgcgt
     6121 tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa atgctccact
     6181 gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc tcaactcgat
     6241 cgaggggatc taccatgagc ccagaacgac gcccggccga catccgccgt gccaccgagg
     6301 cggacatgcc ggcggtctgc accatcgtca accactacat cgagacaagc acggtcaact
     6361 tccgtaccga gccgcaggaa ccgcaggagt aggacggacg acctcgtccg tctgcgggag
     6421 cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggt
     6481 ccctggaagg cacgcaacgc ctacgactgg acggccgagt cgaccgtgta cgtctccccc
     6541 cgccaccagc ggacgggact gggctccacg ctctacaccc acctgctgaa gtccctggag
     6601 gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc
     6661 atgcacgagg cgctcggata tgccccccgc ggcatgctgc gggcggccgg cttcaagcac
     6721 gggaactggc atgacgtggg tttctggcag ctggacttca gcctgccggt gccgccccgt
     6781 ccggtcctgc ccgtcaccga aatctgatga cccctagagt caagcagatc gttcaaacat
     6841 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     6901 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     6961 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     7021 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     7081 accggcatgc aagctgattg cagagctttc gttcgtatca tcggtttcga caacgttcgt
     7141 caagttcaat gcatcagttt cattgcgcac acaccagaat cctactgagt tcgagtatta
     7201 tggcattggg aaacatgttt ttcttgtacc atttgttgtg cttgtaattt actgtgtttt
     7261 ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag ttaatgaatg
     7321 atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt atttgttgtg
     7381 tgttgaattt gaaaatataa gagatatgca aacattttgt tttgagtaaa aatgtgtcaa
     7441 atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag ttgtaccatt
     7501 atgcttattc actaggcaac aaatatattt tcagacctag aaaagctgca aatgttactg
     7561 aatacaagta tgtcctcttg tgttttagac atttatgaac tttcctttat gtaattttcc
     7621 agaatccttg tcagattcta atcattgctt tataattata gttatactca tggatttgta
     7681 gttgagtatg aaaatatttt ttaatgcatt ttatgacttg ccaattgatt gacaacatgc
     7741 atcaatcgaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7801 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     7861 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc
     7921 agcttgccaa tcgaggccga ccaaccgcaa gcgttgtcag tgttgcaaag cgctctgtgt
     7981 gggcctactt taattgcttc cagtgttaaa ttggcgaaag gcaataatat cgcaaaatat
     8041 tgtgttgtaa aatgtaatta tgttttaatt tcatggaaat gtttgagcat aatttttatt
     8101 aatgtactaa attactgttt tgttaaatgc aattttgctt tctcgggatt ttaatatcaa
     8161 aatctattta gaaatacaca atattttgtt gcaggcttgc tggagaatcg atctgctatc
     8221 ataaaaatta caaaaaaatt ttatttgcct caattatttt aggattggta ttaaggacgc
     8281 ttaaattatt tgtcgggtca ctacgcatca ttgtgattga gaagatcagc gatacgaaat
     8341 attcgtagta ctatcgataa tttatttgaa aattcataag aaaagcaaac gttacatgaa
     8401 ttgatgaaac aatacaaaga cagataaagc cacgcacatt taggatattg gccgagatta
     8461 ctgaatattg agtaagatca cggaatttct gacaggagca tgtcttcaat tcagcccaaa
     8521 tggcagttga aatactcaaa ccgccccata tgcaggagcg gatcattcat tgtttgtttg
     8581 gttgcctttg ccaacatggg agtccaaggt ttcagggaag ctggaattcc ggggtgggcg
     8641 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg
     8701 attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg
     8761 ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa
     8821 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga
     8881 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt
     8941 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat
     9001 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt
     9061 cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt
     9121 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc
     9181 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca
     9241 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct
     9301 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca
     9361 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgga
     9421 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
     9481 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
     9541 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     9601 gaaacgatcc ggggaattcg ctagagtcga tttggtgtat cgagattggt tatgaaattc
     9661 agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa ggctatttat
     9721 ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc gttcttgctg
     9781 taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg tatctattca
     9841 aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat acccgcgaaa
     9901 ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag tagaaaaata
     9961 aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat ctcaatataa
    10021 acaaagacgg ccacaagaaa aaaccaaaac accgatattc attaatctta tctagtttct
    10081 caaaaaaatt catatcttcc acacgtgaaa atgccaattt ctcagaccta cctcggctct
    10141 gcgaaggccc ccgctggtat caaaagtttt tatttcatcc gacatggcgc gaccgacctc
    10201 aacgagaagg aaattgtcgt gaacggtgag aagctctggg gcgtgcaagg ttccggaacg
    10261 aacatcggtc tcaatgcaaa aggggaacgc caggctctgt tggcccctcg aaattcggcg
    10321 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    10381 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    10441 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
    10501 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
    10561 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
    10621 agagcgttgc tgcctgtgat caattcgggc acgaacccag tggacataag cctcgttcgg
    10681 ttcgtaagct gtaatgcaag tagcgtaact gccgtcacgc aactggtcca gaaccttgac
    10741 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttcttgtt atgacatgtt
    10801 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
    10861 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
    10921 agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
    10981 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
    11041 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
    11101 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
    11161 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
    11221 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
    11281 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
    11341 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
    11401 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
    11461 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
    11521 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
    11581 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
    11641 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
    11701 tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc
    11761 gccggcgtta actcaagcga ttagatgcac taagcacata attgctcaca gccaaactat
    11821 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
    11881 gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    11941 cccg
//
