LOCUS       40924_20021        8719 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGPDAngls2a, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8719)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8719)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(403..947)
                     /label=trpC terminator
                     /note="trpC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(1025..1105)
                     /label=3' UTR of A. niger gls2a
                     /note="3' UTR of A. niger gls2a"
     exon            complement(1106..3170)
                     /note="exon 3 of A. niger gls2a"
     intron          complement(3171..3224)
                     /note="intron 2 of A. niger gls2a"
     exon            complement(3225..3966)
                     /note="exon 2 of A. niger gls2a"
     intron          complement(3967..4030)
                     /note="intron 1 of A. niger gls2a"
     exon            complement(4031..4097)
                     /note="exon 1 of A. niger gls2a"
     misc_feature    complement(4098..4183)
                     /label=5' UTR of A. niger gls2a
                     /note="5' UTR of A. niger gls2a"
     intron          complement(4196..4311)
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     promoter        complement(4360..6488)
                     /label=gpdA promoter
                     /note="promoter from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     primer_bind     complement(6498..6514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6522..6538)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6546..6576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6591..6612)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6900..7488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7662..8519)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8520..8624)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctctagaaa gaaggattac
      421 ctctaaacaa gtgtacctgt gcattctggg taaacgactc ataggagagt tgtaaaaaag
      481 tttcggccgg cgtattgggt gttacggagc attcactagg caaccatgca tggttactat
      541 tgtataccat cttagtagga antgatttcg aggtttatac ctacgatgaa tgtgtgtcct
      601 gtaggcttga gagttcaagg aagaaacatg caattatctt tgcgaaccca ggngctggtg
      661 acggaatttt catagtcaag ctatcagagt aaagaagagg agcatgtcaa agtacaatta
      721 gagacaaata tatagtcgcg tggagccaag agcggattcc tcagtctcgt aggtctcttg
      781 acgaccgttg atctgcttga tctcgtctcc cgaaaatgaa aatagctctg ctaagctatt
      841 cttctcttcg ccggagcctg naaggcgtta ctaggttgca gtcaatgcat taatgcattg
      901 cagatgagct gtatctggaa gaggtaaacc cgaaaacgcg ttttattgac ttgcttccta
      961 ttctacgact ctagaggatc cccaattcgg ccaaaaaggc caactttttt tttttttttt
     1021 tttttcataa ctaaatacca ttgatgtttg atatatgagc ctcttgcata tatgtatggg
     1081 tgctcaatcc atatcctcgt ctagtctaaa actcaatccg ccatgtcttt ccaatgccga
     1141 cattggggtt cttcaccacc gcatatgcgg ccttgccatc gggctggctg tggtactgca
     1201 tagaggctgt cgaagccgcc gaagctccat cctcaatgac agtcacactg gtcttaccct
     1261 gccattcctt aggaggatca actaccacca cccgctcaac acgaacgttg gccatggtct
     1321 tcaagtactc ggccgtctta ggacccttgg tgccaacatc ctccgagacc agggcagact
     1381 cctggaagcg gaaacggcgg tggatataag ctccacgctc atagtcgaac gtctcaccgt
     1441 catccacgta gagagagcca tcggcttgac cgttcttatc caagaccaca acaagagtgt
     1501 acggatccca tctcatcaag gcgctactgc ggcgaggacg gtccttgcgg gggattacat
     1561 ggccaccctg catcagcaat ggcacagtct ccataggagc aggcaccgta tgccgctttc
     1621 cggctccctg gtagacggtg tagtcaaagt agtcatagta cttttcgtcg tcagcaaggt
     1681 aaatgtcggc cgtggtggcc tcctcggaga caacaggctt agcaagaaga ccggtggagc
     1741 cgagataaag ctggtcgtca atggcaaagc cagcctcatc ccaagggtga gcgtagtact
     1801 gcggcctcac gatcggcatt ccgttcacgg aagcttcgtg gaaggcagtg taccaggcgg
     1861 ggagaagctg atacctcagg cggatagcct gggagatgat agaccggtgt ggctcggcaa
     1921 tcagatacgg ctctctccgg cgcgtgtcaa tatgcgcgtg ggcccggaag aaggggtacc
     1981 aaataccagc ttggtaccat ctggtcaaga gctccttgct agggttctgg aagaaaccgc
     2041 ccacgtcagc accggcaaag gggaagcccg caatgccgtt gttcagaacc atagggatgg
     2101 aagcggccaa gtgttcccaa gtagcctggt tatcacccgt ccacatagca gacatccgtt
     2161 gagcaccagc ataatatgac cgtgtcagaa tgaaaggccg acggatctcg cccttcttgc
     2221 gctctagaag ggcatcgtag gtggcattga ccagggtgat tccatgaacg ttatggatgt
     2281 cacggtgctc ccagttgcca tgatgaaggt tatccttggg catcgtggtt tcgggaccgt
     2341 tgaaaaccga gggctcgttc atgtcgttcc aaatgaagac attggacagc gtccccttga
     2401 acttgtcaaa cttgaataag ctgacccacc atttgatggc ggcggggttg aaggtatcga
     2461 tccagtgaga agagccaggc caacaccacc cgtcgtagat ctcaccgtcc ttgttcttag
     2521 tggccaagtc tttgctcttc atttcttgga cgatgctgta cttgtcctgg ttcttgatgt
     2581 gcgggtcaat gataacgacg agtttgcgct ccgactcatc gagctgctcc tccatgctga
     2641 tcggatcggg gaaactgagt ggatcccagg tgaaatactt tctgtcatcg gtatattcga
     2701 tgtccagcca gatgacatcg taggggatct ggtacttgtc aaagttgcga tcgacctcct
     2761 tgacatcctc atcagtgatg tagttccagc ggcactggtg ataagcaatg gcaaaatgtt
     2821 gaggcaactg agtgtagccg gtgagttcac cataggtctt gctgatttcc tgtggggtag
     2881 gaccaaggaa aacgaacacg tcgagctggc cggactccga aaaccaatga ctctgggtgt
     2941 cagtggtggc gcccacgccg agagcaagag ggttaggaga tgaggtagac ttgacaatgt
     3001 ccacccaggt ctctgcagca ttcagccaga agacaccgac ggtggagtcc ttgcgatgtg
     3061 cctgcatgaa tggaatagca ccatacaagg tcatgggact gctcagctcg tactcaaata
     3121 catccgcatt gtacatgcgg tagggctctt cgtgattccc ttcaccacct ctgggacgct
     3181 gtcagttctg cgagatggaa aatgtcactg cgcgactagc ttaccgagtt tcctttaagg
     3241 agagagagtc agcatgctca ggaattccaa aaacatgctt gtagccaggg aaggtgatat
     3301 ccaatcccac actctcggga cccctgggct tggtgtccgt gtttccacca aagctctcat
     3361 cccaccaagt gctttcatct tcctgggttt gctgctcgcc ctcgccttcg acctctacct
     3421 tagggcgcca atgctccatg ttaaggtagc ccttgttgtt caattgaacg tgggtttggc
     3481 catccctctt gaagtcggcg ctaaaggggg cgtggcggat gacagcctcg aactggttgt
     3541 ccggaccgta caagactctg gtaaagccag actcggtttc aggtctcaag gtcgcggttt
     3601 tgctcaactc caggccacca accaacaccc atttctctgc ctcattgtag cgttccttgc
     3661 gtgctttgct atcgtgtcga agctggatgt caccgttcat gcgcttttcc tcatcgacaa
     3721 caactcgggc ggcgccggac tcgaggaagg agacaacgag aggcagcttg actttctcgt
     3781 tgggggagac ggacttgaga atggttccgt gcaattggcc atccttgaac tggatggagg
     3841 atgagtcgag ttcgtatggg gaggcccagg aggaaccttg ggcggcagca tcgtcggcga
     3901 atgctctgtt ccgcttacag aagcccgatt gagaacatgt cttgaagttc tcgtgtttca
     3961 cggtgactat gcgtcattgt tagatgtcaa ggagagcctc accagtagga gtgtgattgg
     4021 ataggcttac ctccggggat gacaaggcat ccgagtagga tcaccaagga cagcagtagg
     4081 gtccaacggt tggacattgg ccgagttccg gccatgatgg gagagctcag tagtcaatca
     4141 gagacactca acctctgaaa gaagaactat cagcaaccac taggtaccgt gatgtctgct
     4201 caagcggggt agctgttagt caagctgcga tgaagtggga aagctcgaac tgaaaggttc
     4261 aaaggaataa gggatgggaa ggatggagta tggatgtagc aaagtactta cttaggggaa
     4321 ataaaggttc ttggatggga agatgaatat actgaagatg ggaaaagaaa gagaaaagaa
     4381 aagagcagct ggtggggaga gcaggaaaat atggcaacaa atgttggact gacgcaacga
     4441 ccttgtcaac cccgccgaca caccgggcgg acagacgggg caaagctgcc taccagggac
     4501 tgagggacct cagcaggtcg agtgcagagc accggatggg tcgactgcca gcttgtgttc
     4561 ccggtctgcg ccgctggcca gctcctgagc ggcctttccg gtttcataca ccgggcaaag
     4621 caggagaggc acgatatttg gacgccctac agatgccgga tgggccaatt agggagctta
     4681 cgcgccgggt actcgctcta cctacttcgg agaaggtact atctcgtgaa tcttttacca
     4741 gatcggaagc aattggactt ctgtacctag gttaatggca tgctatttcg ccgacggcta
     4801 tacacccctg gcttcacatt ctccttcgct tactgccggt gattcgatga agctccatat
     4861 tctccgatga tgcaatagat tcttggtcaa cgaggggcac accagccttt ccacttcggg
     4921 gcggaggggc ggccggtccc ggattaataa tcatccactg cacctcagag ccgccagagc
     4981 tgtctggcca gtggcttatt actcagccct tctctctgcg tccgtccgtc tctccgcatg
     5041 ccagaaagag tcaccggtca ctgtacagag ctcacgagtt cgtcacattt ttctacaaat
     5101 ggtggaggcg gcggatttta ggctcaagtc atgaccctct gggtcactcc agaatcagct
     5161 aggtcaacga ataaggatga ttctatagga agatccaggc accggtcaac catgatctgg
     5221 acagatttgg gagctcggta taagctctcc acctatctta ttctgtatag tttaggctta
     5281 aagtttatcc aggagatgtt gctgaagtcg atttgagtcc acttcctcac tggtagctat
     5341 acgactttga tggtcgttgt aggggctgta ttaggtctcg atcaaacaca aatagaatta
     5401 aatggtactc gagtccactg aaggtggctt ctccgtcttc cgtagccgtg ccgaaatcct
     5461 tacagcttgt gttgtgtgac ttttggttac gccgtctgac ttttgtggtg agctaactag
     5521 agatcatgct atatctcctg atttaataca atgctcatca taacattcca cctggaactg
     5581 ctagcaacgt ttgacttgca ttgtgcaacg ccctttgcag agctatcgga tgatcaatag
     5641 tgccacgttc taaattcaac caacgcaggt gccccaagcc ttcgacatcc ggatgtattt
     5701 cgaaaacctc atggcgattg cagtcctcag attcatgttc attccaatgc tcattggtga
     5761 ataaaaggtt cacagggaat aagttcaaac tcgagatact tgagaatatt gaaagccaaa
     5821 ggaccctcta tgctccaagc tagagtctca gcctggaaag caaatccaaa tgaagctatg
     5881 ctacctccaa ttcctcatca tcttatctat aatacagagt cgaagaatat cctcttgaca
     5941 ccgctccgtc ctccgacttc aataaggagc ttactcctcc ttgacaccac ccctccagtt
     6001 cttctcggcg ttctggaggg aggccttgtc ggtcttgggc tggccctggc tgagaaagct
     6061 gttggcagcc ttaaagggac gctggaggtc accagtcgct ggcttcccga agacgtggat
     6121 cttaaccaga ttcgaaagcg ccttcagcgg atgatcgact ggatcagaag agcgttggtg
     6181 tacttgaagt acagatgcat gacggccatc atgccaacgc ccatgaactg gctcttaatg
     6241 agctggcgga actggccctt atcgtactcc atgttggtag ttgtgacagg acgaggctcc
     6301 tcgccgcttc caagcggagc aggctcgacg tatttcagtg tcgaaagatc tgcagagatt
     6361 acttcaagtc agccaactgc aaacagaata tcccgccaat agctttggga cgatgcaaga
     6421 tataaacgaa aaagacgaac cgttcttctt attgatttga gcctgtgtgt agagatacaa
     6481 gggaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6541 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6601 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6661 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6721 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6781 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6901 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7321 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7501 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7561 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7621 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7681 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7741 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7801 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7861 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7921 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7981 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8041 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8101 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8161 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8221 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8281 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8341 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8401 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8461 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8521 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8581 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8641 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     8701 atcacgaggc cctttcgtc
//
