LOCUS       40924_20011        7338 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGLAHIL6T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7338)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7338)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      457..1565
                     /label=A. niger glaA promoter
                     /note="A. niger glaA promoter"
                     /regulatory_class="promoter"
     misc_feature    1566..1606
                     /label=5' UTR of A. niger glaA
                     /note="5' UTR of A. niger glaA"
     exon            1607..1820
                     /note="mutated exon 1 of A. niger glaA"
     intron          1821..1895
                     /note="intron 1 of A. niger glaA"
     exon            1896..2182
                     /note="exon 2 of A. niger glaA"
     intron          2183..2237
                     /note="intron 2 of A. niger glaA"
     exon            2238..2334
                     /note="exon 3 of A. niger glaA"
     intron          2335..2395
                     /note="intron 3 of A. niger glaA"
     exon            2396..3033
                     /note="exon 4 of A. niger glaA"
     intron          3034..3091
                     /note="intron 4 of A. niger glaA"
     exon            3092..3775
                     /note="exon 5 of A. niger glaA"
     CDS             3776..3790
                     /codon_start=1
                     /note="unnamed protein product; spMF"
                     /protein_id="SJL89047.1"
                     /translation="RMDKR"
     CDS             3791..4348
                     /codon_start=1
                     /note="unnamed protein product; hIL6"
                     /protein_id="SJL89048.1"
                     /translation="APVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETC
                     NKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQN
                     RFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMT
                     THLILRSFKEFLQSSLRALRQM"
     terminator      4530..5095
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
     primer_bind     complement(5117..5133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5141..5157)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5165..5195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5210..5231)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5519..6107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6281..7138)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7139..7243)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttggctgc aggtcgacta
      421 cctggcctca tacttcttct atgacgctag ccgagagcag cttgaagaac tagtggccct
      481 gtacccagac accaccacgt acgggtctcc gttcaggaca ggcgcggcca acaactggta
      541 tccgcaattt aagcgattgg ccgccattct cggcgacttg gtcttcacca ttacccggcg
      601 ggcattcctc tcgtatgcag aggaaatctc ccctgatctt ccgaactggt cgtacctggc
      661 gacctatgac tatggcaccc cagttctggg gaccttccac ggaagtgacc tgctgcaggt
      721 gttctatggg atcaagccaa actatgcagc tagttctagc cacacgtact atctgagctt
      781 tgtgtatacg ctggatccga actccaaccg gggggagtac attgagtggc cgcagtggaa
      841 ggaatcgcgg cagttgatga atttcggagc gaacgacgcc agtctcctta cggatgattt
      901 ccgcaacggg acatatgagt tcatcctgca gaataccgcg gcgttccaca tctgatgcca
      961 ttggcggagg ggtccggacg gtcaggaact tagccttatg agatgaatga tggacgtgtc
     1021 tggcctcgga aaaggatata tggggatcat gatagtacta gccatattaa tgaagggcat
     1081 ataccacgcg ttggacctgc gttatagctt cccgttagtt atagtaccat cgttatacca
     1141 gccaatcaag tcaccacgca cgaccgggga cggcgaatcc ccgggaattg aaagaaattg
     1201 catcccaggc cagtgaggcc agcgattggc cacctctcca aggcacaggg ccattctgca
     1261 gcgctggtgg attcatcgca atttcccccg gcccggcccg acaccgctat aggctggttc
     1321 tcccacacca tcggagattc gtcgcctaat gtctcgtccg ttcacaagct gaagagcttg
     1381 aagtggcgag atgtctctgc aggaattcaa gctagatgct aagcgatatt gcatggcaat
     1441 atgtgttgat gcatgtgctt cttccttcag cttcccctcg tgcagatgag gtttggctat
     1501 aaattgaagt ggttggtcgg ggttccgtga ggggctgaag tgcttcctcc cttttagacg
     1561 caactgagag cctgagcttc atccccagca tcattacacc tcagcaatgt cgttccgatc
     1621 tctactcgcc ctgagcggcc tcgtctgcac agggttggca aatgtgattt ccaagcgcgc
     1681 gaccttggat tcatggttga gcaacgaagc gaccgtggct cgtactgcca tcctgaataa
     1741 catcggggcg gacggtgctt gggtgtcggg cgcggactct ggcattgtcg ttgctagtcc
     1801 cagcacggat aacccggact gtatgtttcg agctcagatt tagtatgagt gtgtcattga
     1861 ttgattgatg ctgactggcg tgtcgtttgt tgtagacttc tacacctgga ctcgcgactc
     1921 tggtctcgtc ctcaagaccc tcgtcgatct cttccgaaat ggagatacca gtctcctctc
     1981 caccattgag aactacatct ccgcccaggc aattgtccag ggtatcagta acccctctgg
     2041 tgatctgtcc agcggcgctg gtctcggtga acccaagttc aatgtcgatg agactgccta
     2101 cactggttct tggggacggc cgcagcgaga tggtccggct ctgagagcaa ctgctatgat
     2161 cggcttcggg cagtggctgc ttgtatgttc tccaccccct tgcgtctgat ctgtgacata
     2221 tgtagctgac tggtcaggac aatggctaca ccagcaccgc aacggacatt gtttggcccc
     2281 tcgttaggaa cgacctgtcg tatgtggctc aatactggaa ccagacagga tatggtgtgt
     2341 ttgttttatt ttaaatttcc aaagatgcgc cagcagagct aacccgcgat cgcagatctc
     2401 tgggaagaag tcaatggctc gtctttcttt acgattgctg tgcaacaccg cgcccttgtc
     2461 gaaggtagtg ccttcgcgac ggccgtcggc tcgtcctgct cctggtgtga ttctcaggca
     2521 cccgaaattc tctgctacct gcagtccttc tggaccggca gcttcattct ggccaacttc
     2581 gatagcagcc gttccggcaa ggacgcaaac accctcctgg gaagcatcca cacctttgat
     2641 cctgaggccg catgcgacga ctccaccttc cagccctgct ccccgcgcgc gctcgccaac
     2701 cacaaggagg ttgtagactc tttccgctca atctataccc tcaacgatgg tctcagtgac
     2761 agcgaggctg ttgcggtggg tcggtaccct gaggacacgt actacaacgg caacccgtgg
     2821 ttcctgtgca ccttggctgc cgcagagcag ttgtacgatg ctctatacca gtgggacaag
     2881 caggggtcgt tggaggtcac agatgtgtcg ctggacttct tcaaggcact gtacagcgat
     2941 gctgctactg gcacctactc ttcgtccagt tcgacttata gtagcattgt agatgccgtg
     3001 aagactttcg ccgatggctt cgtctctatt gtggtaagtc tacgctagac aagcgctcat
     3061 gttgacagag ggtgcgtact aacagaagta ggaaactcac gccgcaagca acggctccat
     3121 gtccgagcaa tacgacaagt ctgatggcga gcagctttcc gctcgcgacc tgacctggtc
     3181 ttatgctgct ctgctgaccg ccaacaaccg tcgtaactcc gtcgtgcctg cttcttgggg
     3241 cgagacctct gccagcagcg tgcccggcac ctgtgcggcc acatctgcca ttggtaccta
     3301 cagcagtgtg actgtcacct cgtggccgag tatcgtggct actggcggca ccactacgac
     3361 ggctaccccc actggatccg gcagcgtgac ctcgaccagc aagaccaccg cgactgctag
     3421 caagaccagc accagtacgt catcaacctc ctgtaccact cccaccgccg tggctgtgac
     3481 tttcgatctg acagctacca ccacctacgg cgagaacatc tacctggtcg gatcgatctc
     3541 tcagctgggt gactgggaaa ccagcgacgg catagctctg agtgctgaca agtacacttc
     3601 cagcgacccg ctctggtatg tcactgtgac tctgccggct ggtgagtcgt ttgagtacaa
     3661 gtttatccgc attgagagcg atgactccgt ggagtgggag agtgatccca accgagaata
     3721 caccgttcct caggcgtgcg gaacgtcgac cgcgacggtg actgacacct ggaggcgaat
     3781 ggataaaagg gcccctgtac ccccaggaga agattccaaa gatgtagccg ccccacacag
     3841 acagccactc acctcttcag aacgaattga caaacaaatt cggtacatcc tcgacggcat
     3901 ctcagccctg agaaaggaga catgtaacaa gagtaacatg tgtgaaagca gcaaagaggc
     3961 actggcagaa aacaacctga accttccaaa gatggctgaa aaagatggat gcttccaatc
     4021 tggattcaat gaggagactt gcctggtgaa aatcatcact ggtcttttgg agtttgaggt
     4081 atacctagag tacctccaga acagatttga gagtagtgag gaacaagcca gagctgtgca
     4141 gatgagtaca aaagtcctga tccagttcct gcagaaaaag gcaaagaatc tagatgcaat
     4201 aaccacccct gacccaacca caaatgccag cctgctgacg aagctgcagg cacagaacca
     4261 gtggctgcag gacatgacaa ctcatctcat tctgcgcagc tttaaggagt tcctgcagtc
     4321 cagcctgagg gctcttcggc aaatgtagca tgggcacgac ctgcagccaa gcttgagatc
     4381 cacttaacgt tactgaaatc atcaaacagc ttgacgaatc tggatataag atcgttggtg
     4441 tcgatgtcag ctccggagtt gagacaaatg gtgttcagga tctcgataag atacgttcat
     4501 ttgtccaagc agcaaagagt gccttctagt gatttaatag ctccatgtca acaagaataa
     4561 aacgcgtttt cgggtttacc tcttccagat acagctcatc tgcaatgcat taatgcattg
     4621 actgcaacct agtaacgcct tncaggctcc ggcgaagaga agaatagctt agcagagcta
     4681 ttttcatttt cgggagacga gatcaagcag atcaacggtc gtcaagagac ctacgagact
     4741 gaggaatccg ctcttggctc cacgcgacta tatatttgtc tctaattgta ctttgacatg
     4801 ctcctcttct ttactctgat agcttgacta tgaaaattcc gtcaccagcn cctgggttcg
     4861 caaagataat tgcatgtttc ttccttgaac tctcaagcct acaggacaca cattcatcgt
     4921 aggtataaac ctcgaaatca nttcctacta agatggtata caatagtaac catgcatggt
     4981 tgcctagtga atgctccgta acacccaata cgccggccga aactttttta caactctcct
     5041 atgagtcgtt tacccagaat gcacaggtac acttgtttag aggtaatcct tctttctagg
     5101 ggaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5941 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7261 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     7321 tcacgaggcc ctttcgtc
//
