LOCUS       40924_20006       14831 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway vector pFGCGW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14831)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     RNA interference-mediated gene knockdown within specific cell types
  JOURNAL   Plant Mol. Biol. 80 (2), 169-176 (2012)
  PUBMED    22740284
REFERENCE   2  (bases 1 to 14831)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     Gateway-adapted T-DNA binary vectors derived from pFGC5941 
            (AY310901)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 14831)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2005) Department of Plant Sciences, University of 
            Arizona, 303 Forbes Building, Tucson, AZ 85721-0036, USA
REFERENCE   4  (bases 1 to 14831)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 14831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2012"; volume: "80"; issue: "2"; pages: "169-176"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2005) Department of Plant Sciences, University of Arizona, 
            303 Forbes Building, Tucson, AZ 85721-0036, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(376..924)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2305..2650
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2680..2734
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    2765..2884
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2914..2944
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2998..3654
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3999..4301
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4345..4469)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          4480..5828
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     protein_bind    5875..5999
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6043..6345)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(6690..7346)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(7400..7430)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(7460..7579)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      7603..8310
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(8335..8351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8554..8578
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9878..10504
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10941..12005
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      12074..12268
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    12612..12752
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(12938..13526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13616..14407)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agttagatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactag ctcagatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagactcc tttgccccgg agattacaat ggacgatttc
     1741 ctctatcttt acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc
     1801 actgataatg agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
     1861 agagaggcct acgcagcagg tctcatcaag acgatctacc cgagtaacaa tctccaggag
     1921 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc
     1981 aagaacacag agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt
     2041 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
     2101 cctactgaat ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact
     2161 cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
     2221 aatcttcgtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
     2281 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
     2341 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
     2401 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
     2461 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     2521 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
     2581 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     2641 ggagaggaca cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa
     2701 caacaaacaa cattacaatt acatttacaa ttaccatggg gcgcgcccaa tcgatgattt
     2761 atcaacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     2821 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     2881 gtcatattgg cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2941 aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
     3001 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     3061 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     3121 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     3181 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
     3241 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     3301 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     3361 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     3421 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     3481 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     3541 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
     3601 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga
     3661 tctggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
     3721 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
     3781 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     3841 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     3901 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     3961 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
     4021 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     4081 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
     4141 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     4201 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     4261 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
     4321 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
     4381 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     4441 tctcgttcag ctttcttgta caaagtggtt gataaatgtg taagaatttc ttatgttaca
     4501 ttattacatt caacgtttta tcttaattgg ctcttcattt gattgaaatt tgacaattat
     4561 ttcttgtttt tttttttgtc acactctttt tgggttgggg tggccgacga attgtgggaa
     4621 ggtagaaaga ggggaggact tttgttatac tccattagta attactgttt ccgtttcaat
     4681 ttatgtgaca atatttcctt tttagtcggt tccaaaagaa aatgtcagca ttataaacaa
     4741 tttaattttg aaattacaat tttgccatta ataaaatgat ttacaaccac aaaagtatct
     4801 atgagcctgt ttgggtgggc ttataagcag cttattttaa gtggcttata agtcaaaaag
     4861 tgacantttt tgagaagtta gaaaatccta acttctcaaa aagtagcttt taagccactt
     4921 atgacttata agtccaaaaa tttttaagtt accaaacata tattaatggg tttataagct
     4981 tataagccac ttttaagctc acccaaacgg gttctatgtc tcactttaga ctacaaattt
     5041 taaaagtctt catttatttc ttaatctccg tggcgagtna aactataaca cataaagtga
     5101 aacggaggga ataagatgga gtcataaact aatccaaatc tatactctct ccgttaattt
     5161 gttttttagt ttgatttggt acattaataa aacagatttt tcgaaggtta taaacacaga
     5221 cagatgtttc ccagcgagct agcaaaattc caagatttct gtcgaaaatt cgtgtgtttc
     5281 tagctagtac ttgatgttat ctttaacctt ttagtaattt tttgtccttt tctttctatt
     5341 tttcatctta caatgaatta tgagcaagtt ccttaagtag catcacacgt gagatgtttt
     5401 ttatgatatt gactaaatcc aatctttacc attccttaac tagtaaaata caacacatgt
     5461 taattgatac attgcttaac actgaggtta gaaaatttta gaaattagtt gtccaaatgc
     5521 tttgaaatta gaaatcttta atcccttatt tttttttaaa atgttttttc tcactccaaa
     5581 gaaagagaaa ctgacatgaa agctcaaaag atcatgaatc ttactaactt tgtggaacta
     5641 aatgtacatc agaatgtttc tgacatgtga aaatgaaagc tcttaatttt cttcttttat
     5701 ttattgaggg tttttgcatg ctatgcattc aatttgagta ctttaaagca cctataaaca
     5761 cttacttaca cttgccttgg agtttatgtt ttagtgtttt cttcacatct tttttggtca
     5821 atttgcaggt atttggatcc taggtgagtc tagagagtta attaagaccc atcaaccact
     5881 ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     5941 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     6001 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag
     6061 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
     6121 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
     6181 atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag
     6241 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
     6301 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
     6361 tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct
     6421 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc
     6481 ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc
     6541 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac
     6601 atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg
     6661 gcttttagta agccggatcc agatctttac gccccgccct gccactcatc gcagtactgt
     6721 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     6781 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     6841 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     6901 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     6961 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     7021 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     7081 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
     7141 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     7201 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     7261 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     7321 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
     7381 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7441 cctaatgcgg ccgccaatat gactggatat gttgtgtttt acagtattat gtagtctgtt
     7501 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     7561 cttttttgta caaacttgtt gatgggacta gtccctagag tcctgcttta atgagatatg
     7621 cgagacgcct atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac
     7681 ctgagcatgt gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca
     7741 cccgttacta tcgtattttt atgaataata ttctccgttc aatttactga ttgtacccta
     7801 ctacttatat gtacaatatt aaaatgaaaa caatatattg tgctgaatag gtttatagcg
     7861 acatctatga tagagcgcca caataacaaa caattgcgtt ttattattac aaatccaatt
     7921 ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga ttacataaat cttattcaaa
     7981 tttcaaaagt gccccagggg ctagtatcta cgacacaccg agcggcgaac taataacgct
     8041 cactgaaggg aactccggtt ccccgccggc gcgcatgggt gagattcctt gaagttgagt
     8101 attggccgtc cgctctaccg aaagttacgg gcaccattca acccggtcca gcacggcggc
     8161 cgggtaaccg acttgctgcc ccgagaatta tgcagcattt ttttggtgta tgtgggcccc
     8221 aaatgaagtg caggtcaaac cttgacagtg acgacaaatc gttgggcggg tccagggcga
     8281 attttgcgac aacatgtcga ggctcagcag gacctgcagg catgcaagct tggcactggc
     8341 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     8401 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     8461 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt
     8521 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct
     8581 aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc
     8641 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     8701 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     8761 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     8821 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     8881 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     8941 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     9001 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     9061 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     9121 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     9181 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     9241 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     9301 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     9361 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     9421 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     9481 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     9541 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     9601 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     9661 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     9721 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     9781 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     9841 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     9901 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     9961 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
    10021 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
    10081 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
    10141 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
    10201 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
    10261 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
    10321 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
    10381 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
    10441 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
    10501 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
    10561 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
    10621 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
    10681 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
    10741 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
    10801 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
    10861 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa
    10921 gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
    10981 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
    11041 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
    11101 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
    11161 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
    11221 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
    11281 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
    11341 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
    11401 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
    11461 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
    11521 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
    11581 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
    11641 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
    11701 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
    11761 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
    11821 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
    11881 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
    11941 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
    12001 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
    12061 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
    12121 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
    12181 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
    12241 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
    12301 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
    12361 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
    12421 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
    12481 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    12541 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    12601 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
    12661 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    12721 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
    12781 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    12841 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    12901 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    12961 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    13021 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    13081 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    13141 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    13201 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    13261 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    13321 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    13381 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    13441 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    13501 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    13561 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca
    13621 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
    13681 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
    13741 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
    13801 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
    13861 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
    13921 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
    13981 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
    14041 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
    14101 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
    14161 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
    14221 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
    14281 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
    14341 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
    14401 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
    14461 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
    14521 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
    14581 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
    14641 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
    14701 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
    14761 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    14821 tggctggctg g
//
