LOCUS       40924_19986        6080 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGA5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6080)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6080)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      457..1565
                     /label=A. niger glaA promoter
                     /note="A. niger glaA promoter"
                     /regulatory_class="promoter"
     misc_feature    1566..1606
                     /label=5' UTR of A. niger glaA
                     /note="5' UTR of A. niger glaA"
     exon            1607..1820
                     /note="mutated exon 1 of A. niger glaA"
     intron          1821..1895
                     /note="intron 1 of A. niger glaA"
     exon            1896..2182
                     /note="exon 2 of A. niger glaA"
     intron          2183..2237
                     /note="intron 2 of A. niger glaA"
     exon            2238..2334
                     /note="exon 3 of A. niger glaA"
     intron          2335..2395
                     /note="intron 3 of A. niger glaA"
     exon            2396..3033
                     /note="exon 4 of A. niger glaA"
     intron          3034..3091
                     /note="intron 4 of A. niger glaA"
     exon            3092..3778
                     /note="exon 5 of A. niger glaA"
     misc_feature    3779..3834
                     /label=3' UTR of A. niger glaA
                     /note="3' UTR of A. niger glaA"
     primer_bind     complement(3859..3875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3883..3899)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3907..3937)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3952..3973)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4261..4849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5023..5880)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5881..5985)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttggctgc aggtcgacta
      421 cctggcctca tacttcttct atgacgctag ccgagagcag cttgaagaac tagtggccct
      481 gtacccagac accaccacgt acgggtctcc gttcaggaca ggcgcggcca acaactggta
      541 tccgcaattt aagcgattgg ccgccattct cggcgacttg gtcttcacca ttacccggcg
      601 ggcattcctc tcgtatgcag aggaaatctc ccctgatctt ccgaactggt cgtacctggc
      661 gacctatgac tatggcaccc cagttctggg gaccttccac ggaagtgacc tgctgcaggt
      721 gttctatggg atcaagccaa actatgcagc tagttctagc cacacgtact atctgagctt
      781 tgtgtatacg ctggatccga actccaaccg gggggagtac attgagtggc cgcagtggaa
      841 ggaatcgcgg cagttgatga atttcggagc gaacgacgcc agtctcctta cggatgattt
      901 ccgcaacggg acatatgagt tcatcctgca gaataccgcg gcgttccaca tctgatgcca
      961 ttggcggagg ggtccggacg gtcaggaact tagccttatg agatgaatga tggacgtgtc
     1021 tggcctcgga aaaggatata tggggatcat gatagtacta gccatattaa tgaagggcat
     1081 ataccacgcg ttggacctgc gttatagctt cccgttagtt atagtaccat cgttatacca
     1141 gccaatcaag tcaccacgca cgaccgggga cggcgaatcc ccgggaattg aaagaaattg
     1201 catcccaggc cagtgaggcc agcgattggc cacctctcca aggcacaggg ccattctgca
     1261 gcgctggtgg attcatcgca atttcccccg gcccggcccg acaccgctat aggctggttc
     1321 tcccacacca tcggagattc gtcgcctaat gtctcgtccg ttcacaagct gaagagcttg
     1381 aagtggcgag atgtctctgc aggaattcaa gctagatgct aagcgatatt gcatggcaat
     1441 atgtgttgat gcatgtgctt cttccttcag cttcccctcg tgcagatgag gtttggctat
     1501 aaattgaagt ggttggtcgg ggttccgtga ggggctgaag tgcttcctcc cttttagacg
     1561 caactgagag cctgagcttc atccccagca tcattacacc tcagcaatgt cgttccgatc
     1621 tctactcgcc ctgagcggcc tcgtctgcac agggttggcg ggcccgattt ccaagcgcgc
     1681 gaccttggat tcatggttga gcaacgaagc gaccgtggct cgtactgcca tcctgaataa
     1741 catcggggcg gacggtgctt gggtgtcggg cgcggactct ggcattgtcg ttgctagtcc
     1801 cagcacggat aacccggact gtatgtttcg agctcagatt tagtatgagt gtgtcattga
     1861 ttgattgatg ctgactggcg tgtcgtttgt tgtagacttc tacacctgga ctcgcgactc
     1921 tggtctcgtc ctcaagaccc tcgtcgatct cttccgaaat ggagatacca gtctcctctc
     1981 caccattgag aactacatct ccgcccaggc aattgtccag ggtatcagta acccctctgg
     2041 tgatctgtcc agcggcgctg gtctcggtga acccaagttc aatgtcgatg agactgccta
     2101 cactggttct tggggacggc cgcagcgaga tggtccggct ctgagagcaa ctgctatgat
     2161 cggcttcggg cagtggctgc ttgtatgttc tccaccccct tgcgtctgat ctgtgacata
     2221 tgtagctgac tggtcaggac aatggctaca ccagcaccgc aacggacatt gtttggcccc
     2281 tcgttaggaa cgacctgtcg tatgtggctc aatactggaa ccagacagga tatggtgtgt
     2341 ttgttttatt ttaaatttcc aaagatgcgc cagcagagct aacccgcgat cgcagatctc
     2401 tgggaagaag tcaatggctc gtctttcttt acgattgctg tgcaacaccg cgcccttgtc
     2461 gaaggtagtg ccttcgcgac ggccgtcggc tcgtcctgct cctggtgtga ttctcaggca
     2521 cccgaaattc tctgctacct gcagtccttc tggaccggca gcttcattct ggccaacttc
     2581 gatagcagcc gttccggcaa ggacgcaaac accctcctgg gaagcatcca cacctttgat
     2641 cctgaggccg catgcgacga ctccaccttc cagccctgct ccccgcgcgc gctcgccaac
     2701 cacaaggagg ttgtagactc tttccgctca atctataccc tcaacgatgg tctcagtgac
     2761 agcgaggctg ttgcggtggg tcggtaccct gaggacacgt actacaacgg caacccgtgg
     2821 ttcctgtgca ccttggctgc cgcagagcag ttgtacgatg ctctatacca gtgggacaag
     2881 caggggtcgt tggaggtcac agatgtgtcg ctggacttct tcaaggcact gtacagcgat
     2941 gctgctactg gcacctactc ttcgtccagt tcgacttata gtagcattgt agatgccgtg
     3001 aagactttcg ccgatggctt cgtctctatt gtggtaagtc tacgctagac aagcgctcat
     3061 gttgacagag ggtgcgtact aacagaagta ggaaactcac gccgcaagca acggctccat
     3121 gtccgagcaa tacgacaagt ctgatggcga gcagctttcc gctcgcgacc tgacctggtc
     3181 ttatgctgct ctgctgaccg ccaacaaccg tcgtaactcc gtcgtgcctg cttcttgggg
     3241 cgagacctct gccagcagcg tgcccggcac ctgtgcggcc acatctgcca ttggtaccta
     3301 cagcagtgtg actgtcacct cgtggccgag tatcgtggct actggcggca ccactacgac
     3361 ggctaccccc actggatccg gcagcgtgac ctcgaccagc aagaccaccg cgactgctag
     3421 caagaccagc accagtacgt catcaacctc ctgtaccact cccaccgccg tggctgtgac
     3481 tttcgatctg acagctacca ccacctacgg cgagaacatc tacctggtcg gatcgatctc
     3541 tcagctgggt gactgggaaa ccagcgacgg catagctctg agtgctgaca agtacacttc
     3601 cagcgacccg ctctggtatg tcactgtgac tctgccggct ggtgagtcgt ttgagtacaa
     3661 gtttatccgc attgagagcg atgactccgt ggagtgggag agtgatccca accgagaata
     3721 caccgttcct caggcgtgcg gaacgtcgac cgcgacggtg actgacacct ggcggtagac
     3781 aatcaatcca tttcgctata gttaaaggat ggggatgagg gcaattggtt atatgatccc
     3841 cgggaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3901 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3961 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4021 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4081 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4141 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4201 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4261 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4321 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4381 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4441 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4501 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4561 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4621 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4681 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4741 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4801 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4861 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4921 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4981 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5041 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5101 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5161 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5221 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5281 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5341 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5401 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5461 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5521 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5581 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5641 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5701 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5761 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5821 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5881 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5941 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6001 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6061 tatcacgagg ccctttcgtc
//
