LOCUS       40924_19981        4907 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGA4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      457..1565
                     /label=A. niger glaA promoter
                     /note="A. niger glaA promoter"
                     /regulatory_class="promoter"
     misc_feature    1566..1606
                     /label=5' UTR of A. niger glaA
                     /note="5' UTR of A. niger glaA"
     exon            1615..1860
                     /note="part of exon 4 of A. niger glaA"
     intron          1861..1918
                     /note="intron 4 of A. niger glaA"
     exon            1919..2605
                     /note="exon 5 of A. niger glaA"
     misc_feature    2606..2661
                     /label=3' UTR of A. niger glaA
                     /note="3' UTR of A. niger glaA"
     primer_bind     complement(2686..2702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2710..2726)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2734..2764)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2779..2800)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3088..3676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3850..4707)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4708..4812)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttggctgc aggtcgacta
      421 cctggcctca tacttcttct atgacgctag ccgagagcag cttgaagaac tagtggccct
      481 gtacccagac accaccacgt acgggtctcc gttcaggaca ggcgcggcca acaactggta
      541 tccgcaattt aagcgattgg ccgccattct cggcgacttg gtcttcacca ttacccggcg
      601 ggcattcctc tcgtatgcag aggaaatctc ccctgatctt ccgaactggt cgtacctggc
      661 gacctatgac tatggcaccc cagttctggg gaccttccac ggaagtgacc tgctgcaggt
      721 gttctatggg atcaagccaa actatgcagc tagttctagc cacacgtact atctgagctt
      781 tgtgtatacg ctggatccga actccaaccg gggggagtac attgagtggc cgcagtggaa
      841 ggaatcgcgg cagttgatga atttcggagc gaacgacgcc agtctcctta cggatgattt
      901 ccgcaacggg acatatgagt tcatcctgca gaataccgcg gcgttccaca tctgatgcca
      961 ttggcggagg ggtccggacg gtcaggaact tagccttatg agatgaatga tggacgtgtc
     1021 tggcctcgga aaaggatata tggggatcat gatagtacta gccatattaa tgaagggcat
     1081 ataccacgcg ttggacctgc gttatagctt cccgttagtt atagtaccat cgttatacca
     1141 gccaatcaag tcaccacgca cgaccgggga cggcgaatcc ccgggaattg aaagaaattg
     1201 catcccaggc cagtgaggcc agcgattggc cacctctcca aggcacaggg ccattctgca
     1261 gcgctggtgg attcatcgca atttcccccg gcccggcccg acaccgctat aggctggttc
     1321 tcccacacca tcggagattc gtcgcctaat gtctcgtccg ttcacaagct gaagagcttg
     1381 aagtggcgag atgtctctgc aggaattcaa gctagatgct aagcgatatt gcatggcaat
     1441 atgtgttgat gcatgtgctt cttccttcag cttcccctcg tgcagatgag gtttggctat
     1501 aaattgaagt ggttggtcgg ggttccgtga ggggctgaag tgcttcctcc cttttagacg
     1561 caactgagag cctgagcttc atccccagca tcattacacc tcagcacaga tctgcctgag
     1621 gacacgtact acaacggcaa cccgtggttc ctgtgcacct tggctgccgc agagcagttg
     1681 tacgatgctc tataccagtg ggacaagcag gggtcgttgg aggtcacaga tgtgtcgctg
     1741 gacttcttca aggcactgta cagcgatgct gctactggca cctactcttc gtccagttcg
     1801 acttatagta gcattgtaga tgccgtgaag actttcgccg atggcttcgt ctctattgtg
     1861 gtaagtctac gctagacaag cgctcatgtt gacagagggt gcgtactaac agaagtagga
     1921 aactcacgcc gcaagcaacg gctccatgtc cgagcaatac gacaagtctg atggcgagca
     1981 gctttccgct cgcgacctga cctggtctta tgctgctctg ctgaccgcca acaaccgtcg
     2041 taactccgtc gtgcctgctt cttggggcga gacctctgcc agcagcgtgc ccggcacctg
     2101 tgcggccaca tctgccattg gtacctacag cagtgtgact gtcacctcgt ggccgagtat
     2161 cgtggctact ggcggcacca ctacgacggc tacccccact ggatccggca gcgtgacctc
     2221 gaccagcaag accaccgcga ctgctagcaa gaccagcacc agtacgtcat caacctcctg
     2281 taccactccc accgccgtgg ctgtgacttt cgatctgaca gctaccacca cctacggcga
     2341 gaacatctac ctggtcggat cgatctctca gctgggtgac tgggaaacca gcgacggcat
     2401 agctctgagt gctgacaagt acacttccag cgacccgctc tggtatgtca ctgtgactct
     2461 gccggctggt gagtcgtttg agtacaagtt tatccgcatt gagagcgatg actccgtgga
     2521 gtgggagagt gatcccaacc gagaatacac cgttcctcag gcgtgcggaa cgtcgaccgc
     2581 gacggtgact gacacctggc ggtagacaat caatccattt cgctatagtt aaaggatggg
     2641 gatgagggca attggttata tgatccccgg gaattcgtaa tcatggtcat agctgtttcc
     2701 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2761 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2821 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2881 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     2941 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3001 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3061 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3121 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3181 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3241 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3301 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3361 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3421 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3481 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     3541 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3601 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3661 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3721 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3781 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3841 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3901 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3961 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4021 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4081 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4141 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4201 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4261 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4321 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4381 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4441 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4501 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4561 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4621 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4681 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4741 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4801 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     4861 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
