LOCUS       40924_19976        4848 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGA3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4848)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4848)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      457..1565
                     /label=A. niger glaA promoter
                     /note="A. niger glaA promoter"
                     /regulatory_class="promoter"
     misc_feature    1566..1584
                     /label=part of 5' UTR of A. niger glaA
                     /note="part of 5' UTR of A. niger glaA"
     exon            1593..1801
                     /note="part of exon 4 of A. niger glaA"
     intron          1802..1859
                     /note="intron 4 of A. niger glaA"
     exon            1860..2546
                     /note="exon 5 of A. niger glaA"
     misc_feature    2547..2602
                     /label=3' UTR of A. niger glaA
                     /note="3' UTR of A. niger glaA"
     primer_bind     complement(2627..2643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2651..2667)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2675..2705)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2720..2741)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3029..3617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3791..4648)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4649..4753)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttggctgc aggtcgacta
      421 cctggcctca tacttcttct atgacgctag ccgagagcag cttgaagaac tagtggccct
      481 gtacccagac accaccacgt acgggtctcc gttcaggaca ggcgcggcca acaactggta
      541 tccgcaattt aagcgattgg ccgccattct cggcgacttg gtcttcacca ttacccggcg
      601 ggcattcctc tcgtatgcag aggaaatctc ccctgatctt ccgaactggt cgtacctggc
      661 gacctatgac tatggcaccc cagttctggg gaccttccac ggaagtgacc tgctgcaggt
      721 gttctatggg atcaagccaa actatgcagc tagttctagc cacacgtact atctgagctt
      781 tgtgtatacg ctggatccga actccaaccg gggggagtac attgagtggc cgcagtggaa
      841 ggaatcgcgg cagttgatga atttcggagc gaacgacgcc agtctcctta cggatgattt
      901 ccgcaacggg acatatgagt tcatcctgca gaataccgcg gcgttccaca tctgatgcca
      961 ttggcggagg ggtccggacg gtcaggaact tagccttatg agatgaatga tggacgtgtc
     1021 tggcctcgga aaaggatata tggggatcat gatagtacta gccatattaa tgaagggcat
     1081 ataccacgcg ttggacctgc gttatagctt cccgttagtt atagtaccat cgttatacca
     1141 gccaatcaag tcaccacgca cgaccgggga cggcgaatcc ccgggaattg aaagaaattg
     1201 catcccaggc cagtgaggcc agcgattggc cacctctcca aggcacaggg ccattctgca
     1261 gcgctggtgg attcatcgca atttcccccg gcccggcccg acaccgctat aggctggttc
     1321 tcccacacca tcggagattc gtcgcctaat gtctcgtccg ttcacaagct gaagagcttg
     1381 aagtggcgag atgtctctgc aggaattcaa gctagatgct aagcgatatt gcatggcaat
     1441 atgtgttgat gcatgtgctt cttccttcag cttcccctcg tgcagatgag gtttggctat
     1501 aaattgaagt ggttggtcgg ggttccgtga ggggctgaag tgcttcctcc cttttagacg
     1561 caactgagag cctgagcttc atcccagatc tgtgtgcacc ttggctgccg cagagcagtt
     1621 gtacgatgct ctataccagt gggacaagca ggggtcgttg gaggtcacag atgtgtcgct
     1681 ggacttcttc aaggcactgt acagcgatgc tgctactggc acctactctt cgtccagttc
     1741 gacttatagt agcattgtag atgccgtgaa gactttcgcc gatggcttcg tctctattgt
     1801 ggtaagtcta cgctagacaa gcgctcatgt tgacagaggg tgcgtactaa cagaagtagg
     1861 aaactcacgc cgcaagcaac ggctccatgt ccgagcaata cgacaagtct gatggcgagc
     1921 agctttccgc tcgcgacctg acctggtctt atgctgctct gctgaccgcc aacaaccgtc
     1981 gtaactccgt cgtgcctgct tcttggggcg agacctctgc cagcagcgtg cccggcacct
     2041 gtgcggccac atctgccatt ggtacctaca gcagtgtgac tgtcacctcg tggccgagta
     2101 tcgtggctac tggcggcacc actacgacgg ctacccccac tggatccggc agcgtgacct
     2161 cgaccagcaa gaccaccgcg actgctagca agaccagcac cagtacgtca tcaacctcct
     2221 gtaccactcc caccgccgtg gctgtgactt tcgatctgac agctaccacc acctacggcg
     2281 agaacatcta cctggtcgga tcgatctctc agctgggtga ctgggaaacc agcgacggca
     2341 tagctctgag tgctgacaag tacacttcca gcgacccgct ctggtatgtc actgtgactc
     2401 tgccggctgg tgagtcgttt gagtacaagt ttatccgcat tgagagcgat gactccgtgg
     2461 agtgggagag tgatcccaac cgagaataca ccgttcctca ggcgtgcgga acgtcgaccg
     2521 cgacggtgac tgacacctgg cggtagacaa tcaatccatt tcgctatagt taaaggatgg
     2581 ggatgagggc aattggttat atgatccccg ggaattcgta atcatggtca tagctgtttc
     2641 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2701 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2761 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2821 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     3481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4801 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
