LOCUS       40924_19966       10639 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGA1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10639)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10639)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    420..5015
                     /label=5' flanking region of A. niger glaA
                     /note="5' flanking region of A. niger glaA"
     regulatory      5016..6124
                     /label=A. niger glaA promoter
                     /note="A. niger glaA promoter"
                     /regulatory_class="promoter"
     misc_feature    6125..6165
                     /label=5' UTR of A. niger glaA
                     /note="5' UTR of A. niger glaA"
     exon            6166..6379
                     /note="exon 1 of A. niger glaA"
     intron          6380..6454
                     /note="intron 1 of A. niger glaA"
     exon            6455..6741
                     /note="exon 2 of A. niger glaA"
     intron          6742..6796
                     /note="intron 2 of A. niger glaA"
     exon            6797..6893
                     /note="exon 3 of A. niger glaA"
     intron          6894..6954
                     /note="intron 3 of A. niger glaA"
     exon            6955..7592
                     /note="exon 4 of A. niger glaA"
     intron          7593..7650
                     /note="intron 4 of A. niger glaA"
     exon            7651..8337
                     /note="exon 5 of A. niger glaA"
     misc_feature    8338..8393
                     /label=3' UTR of A. niger glaA
                     /note="3' UTR of A. niger glaA"
     primer_bind     complement(8418..8434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8442..8458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8466..8496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8511..8532)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8820..9408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9582..10439)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10440..10544)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttggctgc aggtcgacgg
      421 atcttgcagc aggagagctg cagaacactt caaagatttg cgaacgttgg gccactgatc
      481 ggtctgtcgg gctgatcagc ctgctacttg cggccttggc ttccggtgcg cattattctg
      541 acctggatta catgcaaaga acagagctat gccaggattt tggtacgtaa ccagtatctt
      601 tacctatgca tgtttgacta aacaggagaa gcaaaacgat cctttcaagc tcttcgacta
      661 gccaattttc ttttccgtcc gacgatggat ataatacaag cacttctaat cataggaaac
      721 actctgcaaa acaatggcca gtctgatgca gcatgggttt tgttagggac aacagtccgt
      781 ctcgcgcaga cattaggtct tcacacagaa aagagtgtag cacgcctacc ggatcatgtc
      841 aaatacaaag cacgaaagct atggtacata aaccatgcta caggtaacga cacaagctga
      901 cgcggctaca ggtacactgt cgtttggcaa gattgcctgc tctgtttatg ttacgaccgg
      961 cctcgcgtag tctctatgac cgggtgggct ccagattatt caatcctctc gagcagcgaa
     1021 ctatctttca cagaagctat gtattttcta tgccaaactg ccttaaatat gatcacaaca
     1081 gacggaccgg agatatcgga aaatgcgcga cagcttgaca ttttggccac gattgatagc
     1141 ctcaaccaac gcactcagcc atatctgcgt gaccgccagg aatgcaaaac cctccaacac
     1201 aatctggagc acctggcgtt acgaatgcac atgtctctag ttatttccgt cctgacacgt
     1261 ccagcactga agcgcactgt aatgcaagac gcgtcctatg acatcttgcg cacccgcgcc
     1321 aaattgagcc tgatcgacgc ctctagggcc tttttggatt ttcaggctct gagtgtggta
     1381 cccctccgaa gctggtcaat ggtgcacacg gtgcttagtt ccactttact tctctgcatt
     1441 tgggaggaga cccgaaacga tcccgagtgt cgtgatttac agcaaaaggt gattgaggtc
     1501 ttttctgccg ctggcacagt gggcacagtg gagaacacag catcggagaa tgggcaatgg
     1561 ctatcggaac ggcatatacg agcgctaatc acactgcgca attcggtccg aacggcagtc
     1621 gaacgtgaaa agggggaggc aagcgttggg acagaacgcg cggagcagcc ccagcctttt
     1681 tttcctgtct atgggtatgt gcacccgcta ttgtctgata agtggagctg tgcgatggat
     1741 gctgattttg cagtatgccg aacgggatcc cggatgactt cggtcaagac ttctcaccag
     1801 caagctatct tgactccatt atgaacggta tgctgaggct cccgactatt tatcgatcga
     1861 actaaccgtc gtagtaccca tgtttgactt atcccaagag ctgggttttc tttgagctat
     1921 ccatgacgtt atgcggcccc tcgggaacgg tattcggtgg ttcggaggcc tcggactaaa
     1981 cggcaaaacg gggccacacc aagattgacc ataacgcagg gaagggtccg aagagaaatc
     2041 gaacccagca aaaagctttc aactcaggga cttactgtag ctaccgggtt gccactgggc
     2101 tatatatata cttgacagtc cagcttcctg acggctacca ttgcagtcca tacagtccat
     2161 acatccagac aaagcaaccc acaacgggaa ctttcagtct acgcaattgt gcctaccaca
     2221 acactacata ttccacatca acacatatgt tcaactcact tctatccggt actactacac
     2281 caaactccgg ccgtgcatct cctcccgcca gcgaaatgcc catcgataat gatcacgtgg
     2341 ccgttgcccg tccagctccc cgccgccgcc gcattgtagt agccatgacg ggtgccactg
     2401 gagccatgct cggcatcaaa gtcctaattg ctctgcgccg tctaaatgtg gagacacacc
     2461 tggtgatgag taaatgggcg gaggctacga tcaaatacga gactgactac catccctcaa
     2521 acgtgcgagc gctggccgac tacgtgcaca acatcaatga catggccgcc ccagtatcca
     2581 gcggctcatt ccgcgcggac ggaatgattg tggtaccgtg cagcatgaaa acattggctg
     2641 ctatccactc gggcttttgc gacgatctca tttcaaggac agcagatgtg atgctcaagg
     2701 agcgcaggcg gttggtgcta gtagcgcggg agacgccatt gagcgagatc catctgcgaa
     2761 acatgttgga ggttacacgc gctggggcag tcatcttccc cccagtaccg gcgttctaca
     2821 tcaaggccgg aagtatcgag gacctcatcg accagagtgt tggacgaatg ttggatttat
     2881 ttgacctcga cacgggggat tttgaacgtt ggaatggatg ggaaaaataa gttttcaatt
     2941 tggactgaat tttggcggca tttcttgtat aaaattaaaa ggggcgttga ctggattttg
     3001 gtacttggga tttattctta gctttgactg tacatagttt gggcgtggtt tgatagccga
     3061 cgatcggccg acccacgaac cagatttgca tatgattaca ttccttcaat tttggtccga
     3121 gtcccaaccc gcctttcaac cccaacaact acaagcacgt tgtgtttgct acattgactc
     3181 actcgatatt gctgaaccat gcaggccgcc caactgagtt attacaccaa gcatgcgaat
     3241 cggaaagttc gacaaagcgg gtgaaatgtc cgagttggcg aaccaaagcg caacggtcga
     3301 ggctcctttc cccgcagagg cagccattct gcagcctttg aactgcgggg gaaacggcat
     3361 ttgatcaact cggcactgat gcagtgacca acaggatgtt agcaatgttg gcctaatata
     3421 ttcttactga tctgctgata cgtccctccc ttgcatcagc ctcggtttgc gcatgagaac
     3481 gggttcgaac gttcctgggc cacccggttg gcgtgatatt ctccgcccac gctgtctgtc
     3541 cccctgatgg gaaaccttcg gatcagcgat catcaggcca gttggctatg agaatagggg
     3601 ctttgctgtc ttgatgccta aaatgcaggt ctcatagcaa gatccacagc cgaggaggca
     3661 catggcgatg tcgaaaccca tgggagctga tttttcggtt ctcggatcgt gctccaagac
     3721 atagaaggat attgagcact tgaaacgaag gggtgaaaaa tggaactgta tatagagtta
     3781 ctcccagccc gatccgagag cctatgaccc atagcggtac agatcatggc catcatgaag
     3841 gccctccttt ggctctccct ttccctcgcc gtctgggcga ctccagttca acgggatgca
     3901 gctcctactg tcactattgc gcatccatcg gccaccgtca ttggaaaatc tggcaatgtc
     3961 gagagcttca acaatattcc ctttgcgcag gcccccacag gctcgctgcg tctgaagccc
     4021 ccacaaccct tggaaactgc cctcggcact gttcaggcca caggagcctc gcaatcgtgt
     4081 ccgcagatgt acttcaccac ggatgagagc gaattcccga catcggtcat tggcctcctc
     4141 gctgatctcc ctttggtaca gtcggctacc aatgctctcg aggattgcct gaacattgac
     4201 attcggcgtc cggccgggac caccgcggac tcgaagctgc ctgtgctggt ctggatcttt
     4261 ggcggaggct ttgaacttgg ttcaaaggcg atgtatgatg gtacaacgat ggtatcatcg
     4321 tcgatagaca agaacatgcc tatcgtgttt gtagcaatga attatcgcgt gggaggtttc
     4381 gggttcttgc ccggaaagga gatcctggag gacgggtccg cgaacctagg gctcctggac
     4441 caacgccttg ccctgcagtg ggttgccgac aacatcgagg cctttggtgg agacccggac
     4501 aaggtgacga tttggggaga atcagcagga gccatttccg tttttgatca gatgatcttg
     4561 tacgacggaa acatcactta caaggataag cccttgttcc ggggggccat catggactcc
     4621 ggtagtgttg ttcccgcaga ccccgtcgat ggggtcaagg gacagcaagt atatgatgcg
     4681 gtagtggaat ctgcaggctg ttcctcttct aacgacaccc tagcttgtct gcgtgaacta
     4741 gactacaccg acttcctcaa tgcggcaaac tccgtgccag gcattttaag ctaccattct
     4801 gtggcgttat catatgtgcc tcgaccggac gggacggcgt tgtcggcatc accggacgtt
     4861 ttgggcaaag cagggaaata tgctcgggtc ccgttcatcg tgggcgacca agaggatgag
     4921 gggaccttat tcgccttgtt tcagtccaac attacgacga tcgacgaggt ggtcgactac
     4981 ctggcctcat acttcttcta tgacgctagc cgagagcagc ttgaagaact agtggccctg
     5041 tacccagaca ccaccacgta cgggtctccg ttcaggacag gcgcggccaa caactggtat
     5101 ccgcaattta agcgattggc cgccattctc ggcgacttgg tcttcaccat tacccggcgg
     5161 gcattcctct cgtatgcaga ggaaatctcc cctgatcttc cgaactggtc gtacctggcg
     5221 acctatgact atggcacccc agttctgggg accttccacg gaagtgacct gctgcaggtg
     5281 ttctatggga tcaagccaaa ctatgcagct agttctagcc acacgtacta tctgagcttt
     5341 gtgtatacgc tggatccgaa ctccaaccgg ggggagtaca ttgagtggcc gcagtggaag
     5401 gaatcgcggc agttgatgaa tttcggagcg aacgacgcca gtctccttac ggatgatttc
     5461 cgcaacggga catatgagtt catcctgcag aataccgcgg cgttccacat ctgatgccat
     5521 tggcggaggg gtccggacgg tcaggaactt agccttatga gatgaatgat ggacgtgtct
     5581 ggcctcggaa aaggatatat ggggatcatg atagtactag ccatattaat gaagggcata
     5641 taccacgcgt tggacctgcg ttatagcttc ccgttagtta tagtaccatc gttataccag
     5701 ccaatcaagt caccacgcac gaccggggac ggcgaatccc cgggaattga aagaaattgc
     5761 atcccaggcc agtgaggcca gcgattggcc acctctccaa ggcacagggc cattctgcag
     5821 cgctggtgga ttcatcgcaa tttcccccgg cccggcccga caccgctata ggctggttct
     5881 cccacaccat cggagattcg tcgcctaatg tctcgtccgt tcacaagctg aagagcttga
     5941 agtggcgaga tgtctctgca ggaattcaag ctagatgcta agcgatattg catggcaata
     6001 tgtgttgatg catgtgcttc ttccttcagc ttcccctcgt gcagatgagg tttggctata
     6061 aattgaagtg gttggtcggg gttccgtgag gggctgaagt gcttcctccc ttttagacgc
     6121 aactgagagc ctgagcttca tccccagcat cattacacct cagcaatgtc gttccgatct
     6181 ctactcgccc tgagcggcct cgtctgcaca gggttggcaa atgtgatttc caagcgcgcg
     6241 accttggatt catggttgag caacgaagcg accgtggctc gtactgccat cctgaataac
     6301 atcggggcgg acggtgcttg ggtgtcgggc gcggactctg gcattgtcgt tgctagtccc
     6361 agcacggata acccggactg tatgtttcga gctcagattt agtatgagtg tgtcattgat
     6421 tgattgatgc tgactggcgt gtcgtttgtt gtagacttct acacctggac tcgcgactct
     6481 ggtctcgtcc tcaagaccct cgtcgatctc ttccgaaatg gagataccag tctcctctcc
     6541 accattgaga actacatctc cgcccaggca attgtccagg gtatcagtaa cccctctggt
     6601 gatctgtcca gcggcgctgg tctcggtgaa cccaagttca atgtcgatga gactgcctac
     6661 actggttctt ggggacggcc gcagcgagat ggtccggctc tgagagcaac tgctatgatc
     6721 ggcttcgggc agtggctgct tgtatgttct ccaccccctt gcgtctgatc tgtgacatat
     6781 gtagctgact ggtcaggaca atggctacac cagcaccgca acggacattg tttggcccct
     6841 cgttaggaac gacctgtcgt atgtggctca atactggaac cagacaggat atggtgtgtt
     6901 tgttttattt taaatttcca aagatgcgcc agcagagcta acccgcgatc gcagatctct
     6961 gggaagaagt caatggctcg tctttcttta cgattgctgt gcaacaccgc gcccttgtcg
     7021 aaggtagtgc cttcgcgacg gccgtcggct cgtcctgctc ctggtgtgat tctcaggcac
     7081 ccgaaattct ctgctacctg cagtccttct ggaccggcag cttcattctg gccaacttcg
     7141 atagcagccg ttccggcaag gacgcaaaca ccctcctggg aagcatccac acctttgatc
     7201 ctgaggccgc atgcgacgac tccaccttcc agccctgctc cccgcgcgcg ctcgccaacc
     7261 acaaggaggt tgtagactct ttccgctcaa tctataccct caacgatggt ctcagtgaca
     7321 gcgaggctgt tgcggtgggt cggtaccctg aggacacgta ctacaacggc aacccgtggt
     7381 tcctgtgcac cttggctgcc gcagagcagt tgtacgatgc tctataccag tgggacaagc
     7441 aggggtcgtt ggaggtcaca gatgtgtcgc tggacttctt caaggcactg tacagcgatg
     7501 ctgctactgg cacctactct tcgtccagtt cgacttatag tagcattgta gatgccgtga
     7561 agactttcgc cgatggcttc gtctctattg tggtaagtct acgctagaca agcgctcatg
     7621 ttgacagagg gtgcgtacta acagaagtag gaaactcacg ccgcaagcaa cggctccatg
     7681 tccgagcaat acgacaagtc tgatggcgag cagctttccg ctcgcgacct gacctggtct
     7741 tatgctgctc tgctgaccgc caacaaccgt cgtaactccg tcgtgcctgc ttcttggggc
     7801 gagacctctg ccagcagcgt gcccggcacc tgtgcggcca catctgccat tggtacctac
     7861 agcagtgtga ctgtcacctc gtggccgagt atcgtggcta ctggcggcac cactacgacg
     7921 gctaccccca ctggatccgg cagcgtgacc tcgaccagca agaccaccgc gactgctagc
     7981 aagaccagca ccagtacgtc atcaacctcc tgtaccactc ccaccgccgt ggctgtgact
     8041 ttcgatctga cagctaccac cacctacggc gagaacatct acctggtcgg atcgatctct
     8101 cagctgggtg actgggaaac cagcgacggc atagctctga gtgctgacaa gtacacttcc
     8161 agcgacccgc tctggtatgt cactgtgact ctgccggctg gtgagtcgtt tgagtacaag
     8221 tttatccgca ttgagagcga tgactccgtg gagtgggaga gtgatcccaa ccgagaatac
     8281 accgttcctc aggcgtgcgg aacgtcgacc gcgacggtga ctgacacctg gcggtagaca
     8341 atcaatccat ttcgctatag ttaaaggatg gggatgaggg caattggtta tatgatcccc
     8401 gggaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8461 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8521 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8581 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8641 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8701 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8761 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8821 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8881 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8941 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9001 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9061 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9121 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9181 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9241 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9301 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9361 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9721 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10561 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    10621 atcacgaggc cctttcgtc
//
