LOCUS       V006272                 4004 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006272
VERSION     V006272
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Farzadfard F, Lu TK.
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations
  JOURNAL   Science 346 (6211), 1256272 (2014)
   PUBMED   25395541
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Farzadfard F, Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2014) Biological Engineering, MIT, 77
            Massachusetts Avenue, NE47, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4004)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4004)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science";
            date: "2014"; volume: "346"; issue: "6211"; pages: "1256272"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-DEC-2014) Biological Engineering, MIT, 77 Massachusetts Avenue,
            NE47, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..80
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     misc_feature    89..170
                     /note="primer for the Ec86 RT; msr"
     misc_feature    complement(160..293)
                     /note="template for Ec86 RT; msd(lacZ_ON)"
     CDS             313..1272
                     /gene="ret"
                     /label="Retron Ec86 reverse transcriptase"
                     /note="Retron Ec86 reverse transcriptase from Escherichia
                     coli. Accession#: P23070"
     CDS             1312..2094
                     /label="Beta"
                     /note="single-stranded DNA binding recombinase in the
                     lambda Red system"
     terminator      2115..2201
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2370..2915)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3029..3123)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3147..3803)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3804..3906)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctcgagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 atcagcagga cgcactgacc ttaattaaat gcgcaccctt agcgagaggt ttatcattaa
      121 ggtcaacctc tggatgttgt ttcggcatcc tgcattgaat ctgagttact gtctgttttc
      181 ctgaattcag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      241 tgtgctgcaa ggcgattaag ttgggtgaat tcaggaaacc cgttttttct gacgtaaggg
      301 tgcgcaactt tcatgaaatc cgctgaatat ttgaacactt ttagattgag aaatctcggc
      361 ctacctgtca tgaacaattt gcatgacatg tctaaggcga ctcgcatatc tgttgaaaca
      421 cttcggttgt taatctatac agctgatttt cgctatagga tctacactgt agaaaagaaa
      481 ggcccagaga agagaatgag aaccatttac caaccttctc gagaacttaa agccttacaa
      541 ggatgggttc tacgtaacat tttagataaa ctgtcgtcat ctcctttttc tattggattt
      601 gaaaagcacc aatctatttt gaataatgct accccgcata ttggggcaaa ctttatactg
      661 aatattgatt tggaggattt tttcccaagt ttaactgcta acaaagtttt tggagtgttc
      721 cattctcttg gttataatcg actaatatct tcagttttga caaaaatatg ttgttataaa
      781 aatctgctac cacaaggtgc tccatcatca cctaaattag ctaatctaat atgttctaaa
      841 cttgattatc gtattcaggg ttatgcaggt agtcggggct tgatatatac gagatatgcc
      901 gatgacctca ccttatctgc acagtctatg aaaaaggttg ttaaagcacg tgatttttta
      961 ttttctataa tcccaagtga aggattggtt attaactcaa aaaaaacttg tattagtggg
     1021 cctcgtagtc agaggaaagt tacaggttta gttatttcac aagagaaagt tgggataggt
     1081 agagaaaaat ataaagaaat tagagcaaag atacatcata tattttgcgg taagtcttct
     1141 gagatagaac acgttagggg atggttgtca tttattttaa gtgtggattc aaaaagccat
     1201 aggagattaa taacttatat tagcaaatta gaaaaaaaat atggaaagaa ccctttaaat
     1261 aaagcgaaga cctaaggatc cggttgatat tgattcagag gtataaaacg aatgagtact
     1321 gcactcgcaa cgctggctgg gaagctggct gaacgtgtcg gcatggattc tgtcgaccca
     1381 caggaactga tcaccactct tcgccagacg gcatttaaag gtgatgccag cgatgcgcag
     1441 ttcatcgcat tactgatcgt tgccaaccag tacggcctta atccgtggac gaaagaaatt
     1501 tacgcctttc ctgataagca gaatggcatc gttccggtgg tgggcgttga tggctggtcc
     1561 cgcatcatca atgaaaacca gcagtttgat ggcatggact ttgagcagga caatgaatcc
     1621 tgtacatgcc ggatttaccg caaggaccgt aatcatccga tctgcgttac cgaatggatg
     1681 gatgaatgcc gccgcgaacc attcaaaact cgcgaaggca gagaaatcac ggggccgtgg
     1741 cagtcgcatc ccaaacggat gttacgtcat aaagccatga ttcagtgtgc ccgtctggcc
     1801 ttcggatttg ctggtatcta tgacaaggat gaagccgagc gcattgtcga aaatactgca
     1861 tacactgcag aacgtcagcc ggaacgcgac atcactccgg ttaacgatga aaccatgcag
     1921 gagattaaca ctctgctgat cgccctggat aaaacatggg atgacgactt attgccgctc
     1981 tgttcccaga tatttcgccg cgacattcgt gcatcgtcag aactgacaca ggccgaagca
     2041 gtaaaagctc ttggattcct gaaacagaaa gccgcagagc agaaggtggc agcatgaacg
     2101 cgtgctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2161 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagacctagg
     2221 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     2281 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     2341 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     2401 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     2461 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     2521 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     2581 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     2641 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
     2701 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
     2761 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     2821 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
     2881 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
     2941 aatattacta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
     3001 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     3061 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     3121 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     3181 aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc
     3241 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     3301 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     3361 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     3421 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     3481 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     3541 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
     3601 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     3661 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     3721 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     3781 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     3841 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     3901 cgatcaacgt ctcattttcg ccagatatcg acgtctaaga aaccattatt atcatgacat
     3961 taacctataa aaataggcgt atcacgaggc cctttcgtct tcac
//
