LOCUS       40924_19906        3026 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFF145, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Farzadfard F, Lu TK.
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations
  JOURNAL   Science 346 (6211), 1256272 (2014)
  PUBMED    25395541
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Farzadfard F, Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2014) Biological Engineering, MIT, 77 
            Massachusetts Avenue, NE47, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3026)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2014"; volume: "346"; issue: "6211"; pages: "1256272"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-DEC-2014) Biological Engineering, MIT, 77 Massachusetts Avenue, 
            NE47, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..80
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     RBS             90..101
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             108..890
                     /label=Beta
                     /note="single-stranded DNA binding recombinase in the 
                     lambda Red system"
     terminator      923..1009
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1178..1723)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(1837..1931)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1964..2821)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2822..2926)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 atcagcagga cgcactgacc gaattcatta aagaggagaa aggtaccatg agtactgcac
      121 tcgcaacgct ggctgggaag ctggctgaac gtgtcggcat ggattctgtc gacccacagg
      181 aactgatcac cactcttcgc cagacggcat ttaaaggtga tgccagcgat gcgcagttca
      241 tcgcattact gatcgttgcc aaccagtacg gccttaatcc gtggacgaaa gaaatttacg
      301 cctttcctga taagcagaat ggcatcgttc cggtggtggg cgttgatggc tggtcccgca
      361 tcatcaatga aaaccagcag tttgatggca tggactttga gcaggacaat gaatcctgta
      421 catgccggat ttaccgcaag gaccgtaatc atccgatctg cgttaccgaa tggatggatg
      481 aatgccgccg cgaaccattc aaaactcgcg aaggcagaga aatcacgggg ccgtggcagt
      541 cgcatcccaa acggatgtta cgtcataaag ccatgattca gtgtgcccgt ctggccttcg
      601 gatttgctgg tatctatgac aaggatgaag ccgagcgcat tgtcgaaaat actgcataca
      661 ctgcagaacg tcagccggaa cgcgacatca ctccggttaa cgatgaaacc atgcaggaga
      721 ttaacactct gctgatcgcc ctggataaaa catgggatga cgacttattg ccgctctgtt
      781 cccagatatt tcgccgcgac attcgtgcat cgtcagaact gacacaggcc gaagcagtaa
      841 aagctcttgg attcctgaaa cagaaagccg cagagcagaa ggtggcagca tgaggatccc
      901 atggtacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      961 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
     1021 gacctaggga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     1081 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
     1141 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     1201 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     1261 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
     1321 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
     1381 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     1441 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
     1501 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
     1561 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
     1621 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
     1681 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
     1741 tcagataaaa tattactaga tttcagtgca atttatctct tcaaatgtag cacctgaagt
     1801 cagccccata cgatataagt tgttactagt gcttggattc tcaccaataa aaaacgcccg
     1861 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1921 caacaggagt ccaagcgagc tcgtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2821 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2881 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2941 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3001 gtatcacgag gccctttcgt cttcac
//
